Genetic diversity of 16S rRNA and mcrA genes from methanogenic archaeas
Methanogenic archaeas are found in aquatic and terrestrial environments and are fundamental in the conversion of organic matter into methane, a gas that has a potential use as renewable source of energy, which is also considered as one of the main agents of the greenhouse effect. The vast majority o...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2020-04-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/49877 |
| _version_ | 1817978321969872896 |
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| author | Keyla Vitoria Marques Xavier Eden Silva e Souza Erick de Aquino Santos Michely Correia Diniz |
| author_facet | Keyla Vitoria Marques Xavier Eden Silva e Souza Erick de Aquino Santos Michely Correia Diniz |
| author_sort | Keyla Vitoria Marques Xavier |
| collection | DOAJ |
| description | Methanogenic archaeas are found in aquatic and terrestrial environments and are fundamental in the conversion of organic matter into methane, a gas that has a potential use as renewable source of energy, which is also considered as one of the main agents of the greenhouse effect. The vast majority of microbial genomes can be identified by a conservative molecular marker, the 16S ribosomal gene. However, the mcrA gene have been using in studies of methanogenic archaea diversity as an alternative marker, highly conserved and present only in methanogens. This gene allows the expression of the enzyme Methyl-coenzyme M reductase, the main agent in converting by-products of anaerobic digestion into methane. In this context, we aimed to study the genetic diversity of mcrA and 16S rRNA genes sequences available in databases. The nucleotide sequences were selected from the NCBI. The heterozygosity and molecular diversity indexes were calculated using the Arlequin 3.5 software, with plots generated by package R v3.0. The diversity and heterozygosity indices for both genes may have been influenced by the number and size of the sequences. Descriptive analysis of genetic diversity generated by sequences deposited in databases allowed a detailed study of these molecules. It is known that the organisms in a population are genetically distinct, and that, despite having similarities in their gene composition, the differences are essential for their adaptation to different environments. |
| first_indexed | 2024-04-13T22:27:48Z |
| format | Article |
| id | doaj.art-5adcc84f3bf84af484041a294e51a5cf |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-04-13T22:27:48Z |
| publishDate | 2020-04-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-5adcc84f3bf84af484041a294e51a5cf2022-12-22T02:27:01ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2020-04-0142e49877e4987710.4025/actascibiolsci.v42i1.4987749877Genetic diversity of 16S rRNA and mcrA genes from methanogenic archaeasKeyla Vitoria Marques Xavier0Eden Silva e Souza1Erick de Aquino Santos2Michely Correia Diniz3Universidade Federal de PernambucoUniversidade Federal do Rio Grande do NorteUniversidade Federal da BahiaUniversidade Federal do Vale do São FranciscoMethanogenic archaeas are found in aquatic and terrestrial environments and are fundamental in the conversion of organic matter into methane, a gas that has a potential use as renewable source of energy, which is also considered as one of the main agents of the greenhouse effect. The vast majority of microbial genomes can be identified by a conservative molecular marker, the 16S ribosomal gene. However, the mcrA gene have been using in studies of methanogenic archaea diversity as an alternative marker, highly conserved and present only in methanogens. This gene allows the expression of the enzyme Methyl-coenzyme M reductase, the main agent in converting by-products of anaerobic digestion into methane. In this context, we aimed to study the genetic diversity of mcrA and 16S rRNA genes sequences available in databases. The nucleotide sequences were selected from the NCBI. The heterozygosity and molecular diversity indexes were calculated using the Arlequin 3.5 software, with plots generated by package R v3.0. The diversity and heterozygosity indices for both genes may have been influenced by the number and size of the sequences. Descriptive analysis of genetic diversity generated by sequences deposited in databases allowed a detailed study of these molecules. It is known that the organisms in a population are genetically distinct, and that, despite having similarities in their gene composition, the differences are essential for their adaptation to different environments.http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/49877heterozygosity; microbiomes; methane; anaerobic; arlerquin; databases |
| spellingShingle | Keyla Vitoria Marques Xavier Eden Silva e Souza Erick de Aquino Santos Michely Correia Diniz Genetic diversity of 16S rRNA and mcrA genes from methanogenic archaeas Acta Scientiarum: Biological Sciences heterozygosity; microbiomes; methane; anaerobic; arlerquin; databases |
| title | Genetic diversity of 16S rRNA and mcrA genes from methanogenic archaeas |
| title_full | Genetic diversity of 16S rRNA and mcrA genes from methanogenic archaeas |
| title_fullStr | Genetic diversity of 16S rRNA and mcrA genes from methanogenic archaeas |
| title_full_unstemmed | Genetic diversity of 16S rRNA and mcrA genes from methanogenic archaeas |
| title_short | Genetic diversity of 16S rRNA and mcrA genes from methanogenic archaeas |
| title_sort | genetic diversity of 16s rrna and mcra genes from methanogenic archaeas |
| topic | heterozygosity; microbiomes; methane; anaerobic; arlerquin; databases |
| url | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/49877 |
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