Optimization of short-term storage of pikeperch semen: an applicable approach
Contamination of semen with urine and asynchronous maturation of males and females are main obstacles in artificial reproduction of pikeperch Sander lucioperca. The objective of this study was to overcome these obstacles using optimization of a procedure for short-term storage of pikeperch semen at...
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| Format: | Article |
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Elsevier
2021-01-01
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| Series: | Animal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S175173112030001X |
| _version_ | 1818441263171502080 |
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| author | J. Kristan H. Dadras T. Policar |
| author_facet | J. Kristan H. Dadras T. Policar |
| author_sort | J. Kristan |
| collection | DOAJ |
| description | Contamination of semen with urine and asynchronous maturation of males and females are main obstacles in artificial reproduction of pikeperch Sander lucioperca. The objective of this study was to overcome these obstacles using optimization of a procedure for short-term storage of pikeperch semen at 4 °C using two immobilizing media (IM): (a) IM1, 180 mM NaCl, 2.68 mM KCl, 1.36 mM CaCl2⋅2H2O and 2.38 mM NaHCO3, 343 mOsm/kg; and (b) IM2, 200 mM NaCl, 2.68 mM KCl, 1.36 mM CaCl2⋅2H2O and 2.38 mM NaHCO3, 381 mOsm/kg. Undiluted sperm was used as the control. At 6 h poststorage, there were no substantial changes in spermatozoa motility and velocity at 30 s postactivation in all groups. Over 48 h of storage, the highest spermatozoa motility and velocity were obtained in sperm diluted in IM2 compared to the other groups. IM2 could maintain a significantly higher ATP content of diluted sperm than IM1 and undiluted treatment for 2 days. Similarly, the highest values of eyeing and hatching rates were observed in sperm diluted in IM2 compared to sperm in the other studied groups. It can be concluded that the obtained result is a novel and applicable approach to maintain semen quality of pikeperch during short-term storage, suggesting IM2 as a promising medium for short-term storage. The present study also opens possibilities for ensuring a reliable source of semen as a convenient approach for increasing genetic diversity in hatcheries. |
| first_indexed | 2024-12-14T18:25:28Z |
| format | Article |
| id | doaj.art-5ae39f8ce748422a8f6f7c1ebf045861 |
| institution | Directory Open Access Journal |
| issn | 1751-7311 |
| language | English |
| last_indexed | 2024-12-14T18:25:28Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Animal |
| spelling | doaj.art-5ae39f8ce748422a8f6f7c1ebf0458612022-12-21T22:51:55ZengElsevierAnimal1751-73112021-01-01151100001Optimization of short-term storage of pikeperch semen: an applicable approachJ. Kristan0H. Dadras1T. Policar2Faculty of Fisheries and Protection of Waters, University of South Bohemia, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Zatisi 728, 389 25 Vodnany, Czech RepublicCorresponding author.; Faculty of Fisheries and Protection of Waters, University of South Bohemia, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Zatisi 728, 389 25 Vodnany, Czech RepublicFaculty of Fisheries and Protection of Waters, University of South Bohemia, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Zatisi 728, 389 25 Vodnany, Czech RepublicContamination of semen with urine and asynchronous maturation of males and females are main obstacles in artificial reproduction of pikeperch Sander lucioperca. The objective of this study was to overcome these obstacles using optimization of a procedure for short-term storage of pikeperch semen at 4 °C using two immobilizing media (IM): (a) IM1, 180 mM NaCl, 2.68 mM KCl, 1.36 mM CaCl2⋅2H2O and 2.38 mM NaHCO3, 343 mOsm/kg; and (b) IM2, 200 mM NaCl, 2.68 mM KCl, 1.36 mM CaCl2⋅2H2O and 2.38 mM NaHCO3, 381 mOsm/kg. Undiluted sperm was used as the control. At 6 h poststorage, there were no substantial changes in spermatozoa motility and velocity at 30 s postactivation in all groups. Over 48 h of storage, the highest spermatozoa motility and velocity were obtained in sperm diluted in IM2 compared to the other groups. IM2 could maintain a significantly higher ATP content of diluted sperm than IM1 and undiluted treatment for 2 days. Similarly, the highest values of eyeing and hatching rates were observed in sperm diluted in IM2 compared to sperm in the other studied groups. It can be concluded that the obtained result is a novel and applicable approach to maintain semen quality of pikeperch during short-term storage, suggesting IM2 as a promising medium for short-term storage. The present study also opens possibilities for ensuring a reliable source of semen as a convenient approach for increasing genetic diversity in hatcheries.http://www.sciencedirect.com/science/article/pii/S175173112030001XFishImmobilizing mediaSander luciopercaSperm motility |
| spellingShingle | J. Kristan H. Dadras T. Policar Optimization of short-term storage of pikeperch semen: an applicable approach Animal Fish Immobilizing media Sander lucioperca Sperm motility |
| title | Optimization of short-term storage of pikeperch semen: an applicable approach |
| title_full | Optimization of short-term storage of pikeperch semen: an applicable approach |
| title_fullStr | Optimization of short-term storage of pikeperch semen: an applicable approach |
| title_full_unstemmed | Optimization of short-term storage of pikeperch semen: an applicable approach |
| title_short | Optimization of short-term storage of pikeperch semen: an applicable approach |
| title_sort | optimization of short term storage of pikeperch semen an applicable approach |
| topic | Fish Immobilizing media Sander lucioperca Sperm motility |
| url | http://www.sciencedirect.com/science/article/pii/S175173112030001X |
| work_keys_str_mv | AT jkristan optimizationofshorttermstorageofpikeperchsemenanapplicableapproach AT hdadras optimizationofshorttermstorageofpikeperchsemenanapplicableapproach AT tpolicar optimizationofshorttermstorageofpikeperchsemenanapplicableapproach |