Mucin degrader Akkermansia muciniphila accelerates intestinal stem cell-mediated epithelial development
Mucin-degrading bacteria are densely populated in the intestinal epithelium; however, their interaction with intestinal stem cells (ISCs) and their progeny have not been elucidated. To determine whether mucin-degrading bacteria play a role in gut homeostasis, mice were treated with Akkermansia mucin...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Gut Microbes |
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| Online Access: | http://dx.doi.org/10.1080/19490976.2021.1892441 |
| _version_ | 1818324072026603520 |
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| author | Seungil Kim Yun-Chan Shin Tae-Young Kim Yeji Kim Yong-Soo Lee Su-Hyun Lee Mi-Na Kim Eunju O Kwang Soon Kim Mi-Na Kweon |
| author_facet | Seungil Kim Yun-Chan Shin Tae-Young Kim Yeji Kim Yong-Soo Lee Su-Hyun Lee Mi-Na Kim Eunju O Kwang Soon Kim Mi-Na Kweon |
| author_sort | Seungil Kim |
| collection | DOAJ |
| description | Mucin-degrading bacteria are densely populated in the intestinal epithelium; however, their interaction with intestinal stem cells (ISCs) and their progeny have not been elucidated. To determine whether mucin-degrading bacteria play a role in gut homeostasis, mice were treated with Akkermansia muciniphila, a specialized species that degrades mucin. Administration of A. muciniphila for 4 weeks accelerated the proliferation of Lgr5+ ISCs and promoted the differentiation of Paneth cells and goblet cells in the small intestine (SI). We found similar effects of A. muciniphila in the colon. The levels of acetic and propionic acids were higher in the cecal contents of A. muciniphila-treated mice than in PBS-treated mice. SI organoids treated with cecal contents obtained from A. muciniphila-treated mice were larger and could be diminished by treatment with G protein-coupled receptor (Gpr) 41/43 antagonists. Pre-treatment of mice with A. muciniphila reduced gut damage caused by radiation and methotrexate. Further, a novel isotype of the A. muciniphila strain was isolated from heathy human feces that showed enhanced function in intestinal epithelial regeneration. These findings suggest that mucin-degrading bacteria (e.g., A. muciniphila) may play a crucial role in promoting ISC-mediated epithelial development and contribute to intestinal homeostasis maintenance. |
| first_indexed | 2024-12-13T11:22:46Z |
| format | Article |
| id | doaj.art-5ae4a36dfaa74ef5af2ebe2d86b53e24 |
| institution | Directory Open Access Journal |
| issn | 1949-0976 1949-0984 |
| language | English |
| last_indexed | 2024-12-13T11:22:46Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj.art-5ae4a36dfaa74ef5af2ebe2d86b53e242022-12-21T23:48:25ZengTaylor & Francis GroupGut Microbes1949-09761949-09842021-01-0113110.1080/19490976.2021.18924411892441Mucin degrader Akkermansia muciniphila accelerates intestinal stem cell-mediated epithelial developmentSeungil Kim0Yun-Chan Shin1Tae-Young Kim2Yeji Kim3Yong-Soo Lee4Su-Hyun Lee5Mi-Na Kim6Eunju O7Kwang Soon Kim8Mi-Na Kweon9Asan Medical Center, University of Ulsan College of MedicineAsan Medical Center, University of Ulsan College of MedicineAsan Medical Center, University of Ulsan College of MedicineAsan Medical Center, University of Ulsan College of MedicineAsan Medical Center, University of Ulsan College of MedicineAsan Medical Center, University of Ulsan College of MedicineAsan Medical Center, University of Ulsan College of MedicinePohang University of Science and TechnologyPohang University of Science and TechnologyAsan Medical Center, University of Ulsan College of MedicineMucin-degrading bacteria are densely populated in the intestinal epithelium; however, their interaction with intestinal stem cells (ISCs) and their progeny have not been elucidated. To determine whether mucin-degrading bacteria play a role in gut homeostasis, mice were treated with Akkermansia muciniphila, a specialized species that degrades mucin. Administration of A. muciniphila for 4 weeks accelerated the proliferation of Lgr5+ ISCs and promoted the differentiation of Paneth cells and goblet cells in the small intestine (SI). We found similar effects of A. muciniphila in the colon. The levels of acetic and propionic acids were higher in the cecal contents of A. muciniphila-treated mice than in PBS-treated mice. SI organoids treated with cecal contents obtained from A. muciniphila-treated mice were larger and could be diminished by treatment with G protein-coupled receptor (Gpr) 41/43 antagonists. Pre-treatment of mice with A. muciniphila reduced gut damage caused by radiation and methotrexate. Further, a novel isotype of the A. muciniphila strain was isolated from heathy human feces that showed enhanced function in intestinal epithelial regeneration. These findings suggest that mucin-degrading bacteria (e.g., A. muciniphila) may play a crucial role in promoting ISC-mediated epithelial development and contribute to intestinal homeostasis maintenance.http://dx.doi.org/10.1080/19490976.2021.1892441gut microbiotaakkermansia muciniphilaintestinal stem cellslgr5mucin-degrading bacteriag protein-coupled receptorsepithelial developmentsmall intestineorganoids |
| spellingShingle | Seungil Kim Yun-Chan Shin Tae-Young Kim Yeji Kim Yong-Soo Lee Su-Hyun Lee Mi-Na Kim Eunju O Kwang Soon Kim Mi-Na Kweon Mucin degrader Akkermansia muciniphila accelerates intestinal stem cell-mediated epithelial development Gut Microbes gut microbiota akkermansia muciniphila intestinal stem cells lgr5 mucin-degrading bacteria g protein-coupled receptors epithelial development small intestine organoids |
| title | Mucin degrader Akkermansia muciniphila accelerates intestinal stem cell-mediated epithelial development |
| title_full | Mucin degrader Akkermansia muciniphila accelerates intestinal stem cell-mediated epithelial development |
| title_fullStr | Mucin degrader Akkermansia muciniphila accelerates intestinal stem cell-mediated epithelial development |
| title_full_unstemmed | Mucin degrader Akkermansia muciniphila accelerates intestinal stem cell-mediated epithelial development |
| title_short | Mucin degrader Akkermansia muciniphila accelerates intestinal stem cell-mediated epithelial development |
| title_sort | mucin degrader akkermansia muciniphila accelerates intestinal stem cell mediated epithelial development |
| topic | gut microbiota akkermansia muciniphila intestinal stem cells lgr5 mucin-degrading bacteria g protein-coupled receptors epithelial development small intestine organoids |
| url | http://dx.doi.org/10.1080/19490976.2021.1892441 |
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