Core members and differential abundance of chrysomelid microbiota in the life stages of Podontia affinis (Galerucinae) and adult Silana farinosa (Cassidinae, Coleoptera)
The chrysomelid beetles Podontia affinis and Silana farinosa are members of the subfamilies Galerucinae and Cassidinae, respectively. This study, based on 16S rRNA gene-targeted metagenomics sequencing, reports the core members and differential abundance of bacterial communities in the larvae and ad...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2022-10-01
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| Series: | Biodiversity Data Journal |
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| Online Access: | https://bdj.pensoft.net/article/87459/download/pdf/ |
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| author | Sze-Looi Song Hoi-Sen Yong Kah-Ooi Chua Praphathip Eamsobhana Phaik-Eem Lim Kok-Gan Chan |
| author_facet | Sze-Looi Song Hoi-Sen Yong Kah-Ooi Chua Praphathip Eamsobhana Phaik-Eem Lim Kok-Gan Chan |
| author_sort | Sze-Looi Song |
| collection | DOAJ |
| description | The chrysomelid beetles Podontia affinis and Silana farinosa are members of the subfamilies Galerucinae and Cassidinae, respectively. This study, based on 16S rRNA gene-targeted metagenomics sequencing, reports the core members and differential abundance of bacterial communities in the larvae and adult beetles of P. affinis and the adult S. farinosa. Cyanobacteria/Melainabacteria group was the predominant phylum in the larvae of P. affinis, while Proteobacteria was the predominant phylum in adult P. affinis and S. farinosa. The number of Order, Family, Genus and Species OTUs in the adult stage of P. affinis was higher than that in the larval stage. The bacterial species richness of adult P. affinis was significantly higher than that of adult S. farinosa. Betaproteobacteria was the predominant class in adult P. affinis, Cyanobacteria in the larvae of P. affinis and Gammaproteobacteria in S. farinosa. The larvae and adult beetles of P. affinis and adult S. farinosa had a low number of unique and shared bacterial OTUs (> 5% relative abundance). The differences in the microbiota indicate possible differences in nutrient assimilation, host taxonomy and other stochastic processes. These findings provide new information to our understanding of the bacteria associated with specialist phytophagous chrysomelid beetles and beetles in general. |
| first_indexed | 2024-04-13T16:26:46Z |
| format | Article |
| id | doaj.art-5ae50a6ad1e2431f8d2eea2c5b0d63d7 |
| institution | Directory Open Access Journal |
| issn | 1314-2828 |
| language | English |
| last_indexed | 2024-04-13T16:26:46Z |
| publishDate | 2022-10-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Biodiversity Data Journal |
| spelling | doaj.art-5ae50a6ad1e2431f8d2eea2c5b0d63d72022-12-22T02:39:44ZengPensoft PublishersBiodiversity Data Journal1314-28282022-10-011011410.3897/BDJ.10.e8745987459Core members and differential abundance of chrysomelid microbiota in the life stages of Podontia affinis (Galerucinae) and adult Silana farinosa (Cassidinae, Coleoptera)Sze-Looi Song0Hoi-Sen Yong1Kah-Ooi Chua2Praphathip Eamsobhana3Phaik-Eem Lim4Kok-Gan Chan5Institute of Ocean and Earth Sciences, Universiti MalayaInstitute of Biological Sciences, Faculty of Science, Universiti MalayaCentre for Research in Biotechnology for Agriculture, Universiti MalayaDepartment of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol UniversityInstitute of Ocean and Earth Sciences, Universiti MalayaInstitute of Biological Sciences, Faculty of Science, Universiti MalayaThe chrysomelid beetles Podontia affinis and Silana farinosa are members of the subfamilies Galerucinae and Cassidinae, respectively. This study, based on 16S rRNA gene-targeted metagenomics sequencing, reports the core members and differential abundance of bacterial communities in the larvae and adult beetles of P. affinis and the adult S. farinosa. Cyanobacteria/Melainabacteria group was the predominant phylum in the larvae of P. affinis, while Proteobacteria was the predominant phylum in adult P. affinis and S. farinosa. The number of Order, Family, Genus and Species OTUs in the adult stage of P. affinis was higher than that in the larval stage. The bacterial species richness of adult P. affinis was significantly higher than that of adult S. farinosa. Betaproteobacteria was the predominant class in adult P. affinis, Cyanobacteria in the larvae of P. affinis and Gammaproteobacteria in S. farinosa. The larvae and adult beetles of P. affinis and adult S. farinosa had a low number of unique and shared bacterial OTUs (> 5% relative abundance). The differences in the microbiota indicate possible differences in nutrient assimilation, host taxonomy and other stochastic processes. These findings provide new information to our understanding of the bacteria associated with specialist phytophagous chrysomelid beetles and beetles in general.https://bdj.pensoft.net/article/87459/download/pdf/Bacterial OTUsChrysomelidaephytophagous16S r |
| spellingShingle | Sze-Looi Song Hoi-Sen Yong Kah-Ooi Chua Praphathip Eamsobhana Phaik-Eem Lim Kok-Gan Chan Core members and differential abundance of chrysomelid microbiota in the life stages of Podontia affinis (Galerucinae) and adult Silana farinosa (Cassidinae, Coleoptera) Biodiversity Data Journal Bacterial OTUs Chrysomelidae phytophagous 16S r |
| title | Core members and differential abundance of chrysomelid microbiota in the life stages of Podontia affinis (Galerucinae) and adult Silana farinosa (Cassidinae, Coleoptera) |
| title_full | Core members and differential abundance of chrysomelid microbiota in the life stages of Podontia affinis (Galerucinae) and adult Silana farinosa (Cassidinae, Coleoptera) |
| title_fullStr | Core members and differential abundance of chrysomelid microbiota in the life stages of Podontia affinis (Galerucinae) and adult Silana farinosa (Cassidinae, Coleoptera) |
| title_full_unstemmed | Core members and differential abundance of chrysomelid microbiota in the life stages of Podontia affinis (Galerucinae) and adult Silana farinosa (Cassidinae, Coleoptera) |
| title_short | Core members and differential abundance of chrysomelid microbiota in the life stages of Podontia affinis (Galerucinae) and adult Silana farinosa (Cassidinae, Coleoptera) |
| title_sort | core members and differential abundance of chrysomelid microbiota in the life stages of podontia affinis galerucinae and adult silana farinosa cassidinae coleoptera |
| topic | Bacterial OTUs Chrysomelidae phytophagous 16S r |
| url | https://bdj.pensoft.net/article/87459/download/pdf/ |
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