Induced Pluripotent Stem Cells Generated from P0-Cre;Z/EG Transgenic Mice.
Neural crest (NC) cells are a migratory, multipotent cell population that arises at the neural plate border, and migrate from the dorsal neural tube to their target tissues, where they differentiate into various cell types. Abnormal development of NC cells can result in severe congenital birth defec...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4575135?pdf=render |
| _version_ | 1818948945584324608 |
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| author | Yasuhiro Ogawa Akira Eto Chisato Miyake Nana Tsuchida Haruka Miyake Yasuhiro Takaku Hiroaki Hagiwara Kazuhiko Oishi |
| author_facet | Yasuhiro Ogawa Akira Eto Chisato Miyake Nana Tsuchida Haruka Miyake Yasuhiro Takaku Hiroaki Hagiwara Kazuhiko Oishi |
| author_sort | Yasuhiro Ogawa |
| collection | DOAJ |
| description | Neural crest (NC) cells are a migratory, multipotent cell population that arises at the neural plate border, and migrate from the dorsal neural tube to their target tissues, where they differentiate into various cell types. Abnormal development of NC cells can result in severe congenital birth defects. Because only a limited number of cells can be obtained from an embryo, mechanistic studies are difficult to perform with directly isolated NC cells. Protein zero (P0) is expressed by migrating NC cells during the early embryonic period. In the P0-Cre;Z/EG transgenic mouse, transient activation of the P0 promoter induces Cre-mediated recombination, indelibly tagging NC-derived cells with enhanced green fluorescent protein (EGFP). Induced pluripotent stem cell (iPSC) technology offers new opportunities for both mechanistic studies and development of stem cell-based therapies. Here, we report the generation of iPSCs from the P0-Cre;Z/EG mouse. P0-Cre;Z/EG mouse-derived iPSCs (P/G-iPSCs) exhibited pluripotent stem cell properties. In lineage-directed differentiation studies, P/G-iPSCs were efficiently differentiated along the neural lineage while expressing EGFP. These results suggest that P/G-iPSCs are useful to study NC development and NC-associated diseases. |
| first_indexed | 2024-12-20T08:54:52Z |
| format | Article |
| id | doaj.art-5ae5c43e45df41f994ef7b1fc79b4421 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T08:54:52Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5ae5c43e45df41f994ef7b1fc79b44212022-12-21T19:46:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01109e013862010.1371/journal.pone.0138620Induced Pluripotent Stem Cells Generated from P0-Cre;Z/EG Transgenic Mice.Yasuhiro OgawaAkira EtoChisato MiyakeNana TsuchidaHaruka MiyakeYasuhiro TakakuHiroaki HagiwaraKazuhiko OishiNeural crest (NC) cells are a migratory, multipotent cell population that arises at the neural plate border, and migrate from the dorsal neural tube to their target tissues, where they differentiate into various cell types. Abnormal development of NC cells can result in severe congenital birth defects. Because only a limited number of cells can be obtained from an embryo, mechanistic studies are difficult to perform with directly isolated NC cells. Protein zero (P0) is expressed by migrating NC cells during the early embryonic period. In the P0-Cre;Z/EG transgenic mouse, transient activation of the P0 promoter induces Cre-mediated recombination, indelibly tagging NC-derived cells with enhanced green fluorescent protein (EGFP). Induced pluripotent stem cell (iPSC) technology offers new opportunities for both mechanistic studies and development of stem cell-based therapies. Here, we report the generation of iPSCs from the P0-Cre;Z/EG mouse. P0-Cre;Z/EG mouse-derived iPSCs (P/G-iPSCs) exhibited pluripotent stem cell properties. In lineage-directed differentiation studies, P/G-iPSCs were efficiently differentiated along the neural lineage while expressing EGFP. These results suggest that P/G-iPSCs are useful to study NC development and NC-associated diseases.http://europepmc.org/articles/PMC4575135?pdf=render |
| spellingShingle | Yasuhiro Ogawa Akira Eto Chisato Miyake Nana Tsuchida Haruka Miyake Yasuhiro Takaku Hiroaki Hagiwara Kazuhiko Oishi Induced Pluripotent Stem Cells Generated from P0-Cre;Z/EG Transgenic Mice. PLoS ONE |
| title | Induced Pluripotent Stem Cells Generated from P0-Cre;Z/EG Transgenic Mice. |
| title_full | Induced Pluripotent Stem Cells Generated from P0-Cre;Z/EG Transgenic Mice. |
| title_fullStr | Induced Pluripotent Stem Cells Generated from P0-Cre;Z/EG Transgenic Mice. |
| title_full_unstemmed | Induced Pluripotent Stem Cells Generated from P0-Cre;Z/EG Transgenic Mice. |
| title_short | Induced Pluripotent Stem Cells Generated from P0-Cre;Z/EG Transgenic Mice. |
| title_sort | induced pluripotent stem cells generated from p0 cre z eg transgenic mice |
| url | http://europepmc.org/articles/PMC4575135?pdf=render |
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