New insights on the expression patterns of specific Arabinogalactan proteins in reproductive tissues of Arabidopsis thaliana
Arabinogalactan proteins (AGPs) are hydroxyproline-rich glycoproteins containing a high proportion of carbohydrates, widely distributed in the plant kingdom and ubiquitously present in land plants. AGPs have long been suggested to play important roles in plant reproduction and there is already evide...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1083098/full |
| _version_ | 1811214126505000960 |
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| author | Diana Moreira Diana Moreira Ana Lúcia Lopes Ana Lúcia Lopes Jessy Silva Jessy Silva Maria João Ferreira Maria João Ferreira Sara Cristina Pinto Sara Cristina Pinto Sara Mendes Sara Mendes Luís Gustavo Pereira Luís Gustavo Pereira Sílvia Coimbra Sílvia Coimbra Ana Marta Pereira Ana Marta Pereira |
| author_facet | Diana Moreira Diana Moreira Ana Lúcia Lopes Ana Lúcia Lopes Jessy Silva Jessy Silva Maria João Ferreira Maria João Ferreira Sara Cristina Pinto Sara Cristina Pinto Sara Mendes Sara Mendes Luís Gustavo Pereira Luís Gustavo Pereira Sílvia Coimbra Sílvia Coimbra Ana Marta Pereira Ana Marta Pereira |
| author_sort | Diana Moreira |
| collection | DOAJ |
| description | Arabinogalactan proteins (AGPs) are hydroxyproline-rich glycoproteins containing a high proportion of carbohydrates, widely distributed in the plant kingdom and ubiquitously present in land plants. AGPs have long been suggested to play important roles in plant reproduction and there is already evidence that specific glycoproteins are essential for male and female gametophyte development, pollen tube growth and guidance, and successful fertilization. However, the functions of many of these proteins have yet to be uncovered, mainly due to the difficulty to study individual AGPs. In this work, we generated molecular tools to analyze the expression patterns of a subgroup of individual AGPs in different Arabidopsis tissues, focusing on reproductive processes. This study focused on six AGPs: four classical AGPs (AGP7, AGP25, AGP26, AGP27), one AG peptide (AGP24) and one chimeric AGP (AGP31). These AGPs were first selected based on their predicted expression patterns along the reproductive tissues from available RNA-seq data. Promoter analysis using β-glucuronidase fusions and qPCR in different Arabidopsis tissues allowed to confirm these predictions. AGP7 was mainly expressed in female reproductive tissues, more precisely in the style, funiculus, and integuments near the micropyle region. AGP25 was found to be expressed in the style, septum and ovules with higher expression in the chalaza and funiculus tissues. AGP26 was present in the ovules and pistil valves. AGP27 was expressed in the transmitting tissue, septum and funiculus during seed development. AGP24 was expressed in pollen grains, in mature embryo sacs, with highest expression at the chalazal pole and in the micropyle. AGP31 was expressed in the mature embryo sac with highest expression at the chalaza and, occasionally, in the micropyle. For all these AGPs a co-expression analysis was performed providing new hints on its possible functions. This work confirmed the detection in Arabidopsis male and female tissues of six AGPs never studied before regarding the reproductive process. These results provide novel evidence on the possible involvement of specific AGPs in plant reproduction, as strong candidates to participate in pollen-pistil interactions in an active way, which is significant for this field of study. |
| first_indexed | 2024-04-12T05:58:14Z |
| format | Article |
| id | doaj.art-5ae861df910b40deb3bd7bf6133c814d |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T05:58:14Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-5ae861df910b40deb3bd7bf6133c814d2022-12-22T03:45:06ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-12-011310.3389/fpls.2022.10830981083098New insights on the expression patterns of specific Arabinogalactan proteins in reproductive tissues of Arabidopsis thalianaDiana Moreira0Diana Moreira1Ana Lúcia Lopes2Ana Lúcia Lopes3Jessy Silva4Jessy Silva5Maria João Ferreira6Maria João Ferreira7Sara Cristina Pinto8Sara Cristina Pinto9Sara Mendes10Sara Mendes11Luís Gustavo Pereira12Luís Gustavo Pereira13Sílvia Coimbra14Sílvia Coimbra15Ana Marta Pereira16Ana Marta Pereira17Department of Biology, Faculty of Sciences, University of Porto, Porto, PortugalLaboratório Associado para a Química Verde (LAQV) Requimte, Sustainable Chemistry, University of Porto, Porto, PortugalDepartment of Biology, Faculty of Sciences, University of Porto, Porto, PortugalBiosystems and Integrative Sciences Institute – BioISI, Porto, PortugalLaboratório Associado para a Química Verde (LAQV) Requimte, Sustainable Chemistry, University of Porto, Porto, PortugalDepartment of Biology, University of Minho, Campus de Gualtar, Braga, PortugalDepartment of Biology, Faculty of Sciences, University of Porto, Porto, PortugalLaboratório Associado para a Química Verde (LAQV) Requimte, Sustainable Chemistry, University of Porto, Porto, PortugalDepartment of Biology, Faculty of Sciences, University of Porto, Porto, PortugalLaboratório Associado para a Química Verde (LAQV) Requimte, Sustainable Chemistry, University of Porto, Porto, PortugalDepartment of Biology, Faculty of Sciences, University of Porto, Porto, PortugalLaboratório Associado para a Química Verde (LAQV) Requimte, Sustainable Chemistry, University of Porto, Porto, PortugalDepartment of Biology, Faculty of Sciences, University of Porto, Porto, PortugalGreenUPorto - Sustainable Agrifood Production Research Centre, Universidade do Porto, Porto, PortugalDepartment of Biology, Faculty of Sciences, University of Porto, Porto, PortugalLaboratório Associado para a Química Verde (LAQV) Requimte, Sustainable Chemistry, University of Porto, Porto, PortugalDepartment of Biology, Faculty of Sciences, University of Porto, Porto, PortugalLaboratório Associado para a Química Verde (LAQV) Requimte, Sustainable Chemistry, University of Porto, Porto, PortugalArabinogalactan proteins (AGPs) are hydroxyproline-rich glycoproteins containing a high proportion of carbohydrates, widely distributed in the plant kingdom and ubiquitously present in land plants. AGPs have long been suggested to play important roles in plant reproduction and there is already evidence that specific glycoproteins are essential for male and female gametophyte development, pollen tube growth and guidance, and successful fertilization. However, the functions of many of these proteins have yet to be uncovered, mainly due to the difficulty to study individual AGPs. In this work, we generated molecular tools to analyze the expression patterns of a subgroup of individual AGPs in different Arabidopsis tissues, focusing on reproductive processes. This study focused on six AGPs: four classical AGPs (AGP7, AGP25, AGP26, AGP27), one AG peptide (AGP24) and one chimeric AGP (AGP31). These AGPs were first selected based on their predicted expression patterns along the reproductive tissues from available RNA-seq data. Promoter analysis using β-glucuronidase fusions and qPCR in different Arabidopsis tissues allowed to confirm these predictions. AGP7 was mainly expressed in female reproductive tissues, more precisely in the style, funiculus, and integuments near the micropyle region. AGP25 was found to be expressed in the style, septum and ovules with higher expression in the chalaza and funiculus tissues. AGP26 was present in the ovules and pistil valves. AGP27 was expressed in the transmitting tissue, septum and funiculus during seed development. AGP24 was expressed in pollen grains, in mature embryo sacs, with highest expression at the chalazal pole and in the micropyle. AGP31 was expressed in the mature embryo sac with highest expression at the chalaza and, occasionally, in the micropyle. For all these AGPs a co-expression analysis was performed providing new hints on its possible functions. This work confirmed the detection in Arabidopsis male and female tissues of six AGPs never studied before regarding the reproductive process. These results provide novel evidence on the possible involvement of specific AGPs in plant reproduction, as strong candidates to participate in pollen-pistil interactions in an active way, which is significant for this field of study.https://www.frontiersin.org/articles/10.3389/fpls.2022.1083098/fullArabinogalactan proteinspollen-pistil interactionsfemale gametophytemale gametophyteplant reproduction |
| spellingShingle | Diana Moreira Diana Moreira Ana Lúcia Lopes Ana Lúcia Lopes Jessy Silva Jessy Silva Maria João Ferreira Maria João Ferreira Sara Cristina Pinto Sara Cristina Pinto Sara Mendes Sara Mendes Luís Gustavo Pereira Luís Gustavo Pereira Sílvia Coimbra Sílvia Coimbra Ana Marta Pereira Ana Marta Pereira New insights on the expression patterns of specific Arabinogalactan proteins in reproductive tissues of Arabidopsis thaliana Frontiers in Plant Science Arabinogalactan proteins pollen-pistil interactions female gametophyte male gametophyte plant reproduction |
| title | New insights on the expression patterns of specific Arabinogalactan proteins in reproductive tissues of Arabidopsis thaliana |
| title_full | New insights on the expression patterns of specific Arabinogalactan proteins in reproductive tissues of Arabidopsis thaliana |
| title_fullStr | New insights on the expression patterns of specific Arabinogalactan proteins in reproductive tissues of Arabidopsis thaliana |
| title_full_unstemmed | New insights on the expression patterns of specific Arabinogalactan proteins in reproductive tissues of Arabidopsis thaliana |
| title_short | New insights on the expression patterns of specific Arabinogalactan proteins in reproductive tissues of Arabidopsis thaliana |
| title_sort | new insights on the expression patterns of specific arabinogalactan proteins in reproductive tissues of arabidopsis thaliana |
| topic | Arabinogalactan proteins pollen-pistil interactions female gametophyte male gametophyte plant reproduction |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1083098/full |
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