Integrating lncRNAs and mRNAs Expression Profiles in Penicillin-Induced Persistent Chlamydial Infection in HeLa Cells
Chlamydia trachomatis (C. trachomatis) is a major etiological agent of sexually transmitted infection. Some stressing conditions can result in persistent chlamydial infection, which is thought to be associated with severe complications including ectopic pregnancy and tubal factor infertility. Long n...
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.744901/full |
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| author | Xiaobao Huang Qian Liufu Rui Xu Xiaohong Chen Mingna Liu Jiande Han Hongyu Guan Chunguang Ma |
| author_facet | Xiaobao Huang Qian Liufu Rui Xu Xiaohong Chen Mingna Liu Jiande Han Hongyu Guan Chunguang Ma |
| author_sort | Xiaobao Huang |
| collection | DOAJ |
| description | Chlamydia trachomatis (C. trachomatis) is a major etiological agent of sexually transmitted infection. Some stressing conditions can result in persistent chlamydial infection, which is thought to be associated with severe complications including ectopic pregnancy and tubal factor infertility. Long noncoding RNAs (lncRNAs) have been identified as key modulators in many biological processes. Nevertheless, the role of lncRNAs in persistent chlamydial infection is still unclear. In this study, we used lncRNA and mRNA microarray to identify the global lncRNAs and mRNAs expression in penicillin-induced persistent chlamydial infection in HeLa cells as well as the control group (HeLa cells without C. trachomatis infection). Among 1005 differentially expressed lncRNAs, 585 lncRNAs were upregulated and 420 downregulated in persistent chlamydial infection, while 410 mRNAs were identified to express differentially, of which 113 mRNAs were upregulated and 297 downregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis with differentially expressed genes were performed. We then constructed the lncRNA-miRNA-mRNA competing endogenous RNAs (ceRNAs) network. Four mRNAs were validated to be changed by quantitative real-time PCR which were correlated with the microarray result. Integration of protein-protein interaction network was constructed and hub genes were identified. These findings provide a new perspective on the molecular mechanisms of penicillin-induced persistent chlamydial infection. |
| first_indexed | 2024-12-13T03:35:29Z |
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| id | doaj.art-5aebbeaacf8f43fe88e3db9e2f18f485 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-13T03:35:29Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-5aebbeaacf8f43fe88e3db9e2f18f4852022-12-22T00:01:03ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-02-01910.3389/fmolb.2022.744901744901Integrating lncRNAs and mRNAs Expression Profiles in Penicillin-Induced Persistent Chlamydial Infection in HeLa CellsXiaobao Huang0Qian Liufu1Rui Xu2Xiaohong Chen3Mingna Liu4Jiande Han5Hongyu Guan6Chunguang Ma7Department of Dermatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Dermatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Dermatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Dermatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Dermatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Dermatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Endocrinology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Dermatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaChlamydia trachomatis (C. trachomatis) is a major etiological agent of sexually transmitted infection. Some stressing conditions can result in persistent chlamydial infection, which is thought to be associated with severe complications including ectopic pregnancy and tubal factor infertility. Long noncoding RNAs (lncRNAs) have been identified as key modulators in many biological processes. Nevertheless, the role of lncRNAs in persistent chlamydial infection is still unclear. In this study, we used lncRNA and mRNA microarray to identify the global lncRNAs and mRNAs expression in penicillin-induced persistent chlamydial infection in HeLa cells as well as the control group (HeLa cells without C. trachomatis infection). Among 1005 differentially expressed lncRNAs, 585 lncRNAs were upregulated and 420 downregulated in persistent chlamydial infection, while 410 mRNAs were identified to express differentially, of which 113 mRNAs were upregulated and 297 downregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis with differentially expressed genes were performed. We then constructed the lncRNA-miRNA-mRNA competing endogenous RNAs (ceRNAs) network. Four mRNAs were validated to be changed by quantitative real-time PCR which were correlated with the microarray result. Integration of protein-protein interaction network was constructed and hub genes were identified. These findings provide a new perspective on the molecular mechanisms of penicillin-induced persistent chlamydial infection.https://www.frontiersin.org/articles/10.3389/fmolb.2022.744901/fulllncRNAsmRNAsChlamydia trachomatispersistent infectionmicroarraybioinformatics analysis |
| spellingShingle | Xiaobao Huang Qian Liufu Rui Xu Xiaohong Chen Mingna Liu Jiande Han Hongyu Guan Chunguang Ma Integrating lncRNAs and mRNAs Expression Profiles in Penicillin-Induced Persistent Chlamydial Infection in HeLa Cells Frontiers in Molecular Biosciences lncRNAs mRNAs Chlamydia trachomatis persistent infection microarray bioinformatics analysis |
| title | Integrating lncRNAs and mRNAs Expression Profiles in Penicillin-Induced Persistent Chlamydial Infection in HeLa Cells |
| title_full | Integrating lncRNAs and mRNAs Expression Profiles in Penicillin-Induced Persistent Chlamydial Infection in HeLa Cells |
| title_fullStr | Integrating lncRNAs and mRNAs Expression Profiles in Penicillin-Induced Persistent Chlamydial Infection in HeLa Cells |
| title_full_unstemmed | Integrating lncRNAs and mRNAs Expression Profiles in Penicillin-Induced Persistent Chlamydial Infection in HeLa Cells |
| title_short | Integrating lncRNAs and mRNAs Expression Profiles in Penicillin-Induced Persistent Chlamydial Infection in HeLa Cells |
| title_sort | integrating lncrnas and mrnas expression profiles in penicillin induced persistent chlamydial infection in hela cells |
| topic | lncRNAs mRNAs Chlamydia trachomatis persistent infection microarray bioinformatics analysis |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.744901/full |
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