Effect of the mycotoxin deoxynivalenol on the immune responses of rainbow trout (Oncorhynchus mykiss)
The trichothecene mycotoxin deoxynivalenol (DON) is commonly found as a natural contaminant in cereals such as wheat, barley, and corn, and exhibits various toxicological effects when present in animal feeds. The effects of DON at a nominal 2 mg/kg feed on immune responses of rainbow trout were inve...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2015-09-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-201509-0007_effect-of-the-mycotoxin-deoxynivalenol-on-the-immune-responses-of-rainbow-trout-oncorhynchus-mykiss.php |
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| author | I. Matejova M. Vicenova L. Vojtek H. Kudlackova K. Nedbalcova M. Faldyna E. Sisperova H. Modra Z. Svobodova |
| author_facet | I. Matejova M. Vicenova L. Vojtek H. Kudlackova K. Nedbalcova M. Faldyna E. Sisperova H. Modra Z. Svobodova |
| author_sort | I. Matejova |
| collection | DOAJ |
| description | The trichothecene mycotoxin deoxynivalenol (DON) is commonly found as a natural contaminant in cereals such as wheat, barley, and corn, and exhibits various toxicological effects when present in animal feeds. The effects of DON at a nominal 2 mg/kg feed on immune responses of rainbow trout were investigated, including relative gene expression of important cytokines (TNF-α, IL-8, IL-1β, IL-10), lysozyme concentration in skin mucus, and level of antigen-specific IgM in blood plasma after vaccination with the commercial vaccine AquaVac ERM containing Yersinia ruckeri type 1 (Hagerman strain). Twenty one-year-old rainbow trout Oncorhynchus mykiss were randomly divided into two groups. The control received a commercial feed with a naturally occurring low level of DON (225 μg/kg feed), while an experimental group was fed the same formulation with DON added to 1964 μg/kg feed. The trial continued for 23 days. Consumption of feed with added DON showed a significant effect on the immune system, as indicated by a higher level of pro-inflammatory cytokine TNF-α (P < 0.05) and of IL-8 (non-significant) in head kidney. Expression of the pro-inflammatory gene IL-1β and the expression of a gene encoding anti-inflammatory cytokine (IL-10) were not influenced by DON treatment. Effects on the concentration of skin mucus lysozyme and specific IgM antibody levels were not observed during this experiment. These results suggest that prolonged ingestion of low doses of DON may influence the immune responses of rainbow trout. |
| first_indexed | 2024-04-10T07:59:36Z |
| format | Article |
| id | doaj.art-5aee5ee42a574804bef05ddc99ebb64f |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:59:36Z |
| publishDate | 2015-09-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-5aee5ee42a574804bef05ddc99ebb64f2023-02-23T03:50:24ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922015-09-0160951552110.17221/8443-VETMEDvet-201509-0007Effect of the mycotoxin deoxynivalenol on the immune responses of rainbow trout (Oncorhynchus mykiss)I. Matejova0M. Vicenova1L. Vojtek2H. Kudlackova3K. Nedbalcova4M. Faldyna5E. Sisperova6H. Modra7Z. Svobodova8University of Veterinary and Pharmaceutical Sciences, Brno, Czech RepublicVeterinary Research Institute, Brno, Czech RepublicMasaryk University, Brno, Czech RepublicVeterinary Research Institute, Brno, Czech RepublicVeterinary Research Institute, Brno, Czech RepublicVeterinary Research Institute, Brno, Czech RepublicUniversity of Veterinary and Pharmaceutical Sciences, Brno, Czech RepublicUniversity of Veterinary and Pharmaceutical Sciences, Brno, Czech RepublicUniversity of Veterinary and Pharmaceutical Sciences, Brno, Czech RepublicThe trichothecene mycotoxin deoxynivalenol (DON) is commonly found as a natural contaminant in cereals such as wheat, barley, and corn, and exhibits various toxicological effects when present in animal feeds. The effects of DON at a nominal 2 mg/kg feed on immune responses of rainbow trout were investigated, including relative gene expression of important cytokines (TNF-α, IL-8, IL-1β, IL-10), lysozyme concentration in skin mucus, and level of antigen-specific IgM in blood plasma after vaccination with the commercial vaccine AquaVac ERM containing Yersinia ruckeri type 1 (Hagerman strain). Twenty one-year-old rainbow trout Oncorhynchus mykiss were randomly divided into two groups. The control received a commercial feed with a naturally occurring low level of DON (225 μg/kg feed), while an experimental group was fed the same formulation with DON added to 1964 μg/kg feed. The trial continued for 23 days. Consumption of feed with added DON showed a significant effect on the immune system, as indicated by a higher level of pro-inflammatory cytokine TNF-α (P < 0.05) and of IL-8 (non-significant) in head kidney. Expression of the pro-inflammatory gene IL-1β and the expression of a gene encoding anti-inflammatory cytokine (IL-10) were not influenced by DON treatment. Effects on the concentration of skin mucus lysozyme and specific IgM antibody levels were not observed during this experiment. These results suggest that prolonged ingestion of low doses of DON may influence the immune responses of rainbow trout.https://vetmed.agriculturejournals.cz/artkey/vet-201509-0007_effect-of-the-mycotoxin-deoxynivalenol-on-the-immune-responses-of-rainbow-trout-oncorhynchus-mykiss.phpfishfeedstuffsmycotoxinimmune system |
| spellingShingle | I. Matejova M. Vicenova L. Vojtek H. Kudlackova K. Nedbalcova M. Faldyna E. Sisperova H. Modra Z. Svobodova Effect of the mycotoxin deoxynivalenol on the immune responses of rainbow trout (Oncorhynchus mykiss) Veterinární Medicína fish feedstuffs mycotoxin immune system |
| title | Effect of the mycotoxin deoxynivalenol on the immune responses of rainbow trout (Oncorhynchus mykiss) |
| title_full | Effect of the mycotoxin deoxynivalenol on the immune responses of rainbow trout (Oncorhynchus mykiss) |
| title_fullStr | Effect of the mycotoxin deoxynivalenol on the immune responses of rainbow trout (Oncorhynchus mykiss) |
| title_full_unstemmed | Effect of the mycotoxin deoxynivalenol on the immune responses of rainbow trout (Oncorhynchus mykiss) |
| title_short | Effect of the mycotoxin deoxynivalenol on the immune responses of rainbow trout (Oncorhynchus mykiss) |
| title_sort | effect of the mycotoxin deoxynivalenol on the immune responses of rainbow trout oncorhynchus mykiss |
| topic | fish feedstuffs mycotoxin immune system |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-201509-0007_effect-of-the-mycotoxin-deoxynivalenol-on-the-immune-responses-of-rainbow-trout-oncorhynchus-mykiss.php |
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