Effects of Calcium Sulfate Combined with Platelet-rich Plasma on Restoration of Long Bone Defect in Rabbits
Background: The treatment for long bone defects has been a hot topic in the field of regenerative medicine. This study aimed to evaluate the therapeutic effects of calcium sulfate (CS) combined with platelet-rich plasma (PRP) on long bone defect restoration. Methods: A radial bone defect model was c...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer
2016-01-01
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| Series: | Chinese Medical Journal |
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| Online Access: | http://www.cmj.org/article.asp?issn=0366-6999;year=2016;volume=129;issue=5;spage=557;epage=561;aulast=Chen |
| _version_ | 1818351427888611328 |
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| author | Hua Chen Xin-Ran Ji Qun Zhang Xue-Zhong Tian Bo-Xun Zhang Pei-Fu Tang |
| author_facet | Hua Chen Xin-Ran Ji Qun Zhang Xue-Zhong Tian Bo-Xun Zhang Pei-Fu Tang |
| author_sort | Hua Chen |
| collection | DOAJ |
| description | Background: The treatment for long bone defects has been a hot topic in the field of regenerative medicine. This study aimed to evaluate the therapeutic effects of calcium sulfate (CS) combined with platelet-rich plasma (PRP) on long bone defect restoration.
Methods: A radial bone defect model was constructed through an osteotomy using New Zealand rabbits. The rabbits were randomly divided into four groups (n = 10 in each group): a CS combined with PRP (CS-PRP) group, a CS group, a PRP group, and a positive (recombinant human bone morphogenetic protein-2) control group. PRP was prepared from autologous blood using a two-step centrifugation process. CS-PRP was obtained by mixing hemihydrate CS with PRP. Radiographs and histologic micrographs were generated. The percentage of bone regenerated bone area in each rabbit was calculated at 10 weeks. One-way analysis of variance was performed in this study.
Results: The radiographs and histologic micrographs showed bone restoration in the CS-PRP and positive control groups, while nonunion was observed in the CS and PRP groups. The percentages of bone regenerated bone area in the CS-PRP (84.60 ± 2.87%) and positive control (52.21 ± 4.53%) groups were significantly greater than those in the CS group (12.34 ± 2.17%) and PRP group (16.52 ± 4.22%) (P < 0.001). In addition, the bone strength of CS-PRP group (43.10 ± 4.10%) was significantly greater than that of the CS group (20.10 ± 3.70%) or PRP group (25.10 ± 2.10%) (P < 0.001).
Conclusion: CS-PRP functions as an effective treatment for long bone defects through stimulating bone regeneration and enhancing new bone strength. |
| first_indexed | 2024-12-13T18:37:34Z |
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| institution | Directory Open Access Journal |
| issn | 0366-6999 |
| language | English |
| last_indexed | 2024-12-13T18:37:34Z |
| publishDate | 2016-01-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Chinese Medical Journal |
| spelling | doaj.art-5aeebc0483ab4a90bd67bd47da0a5d042022-12-21T23:35:19ZengWolters KluwerChinese Medical Journal0366-69992016-01-01129555756110.4103/0366-6999.176981Effects of Calcium Sulfate Combined with Platelet-rich Plasma on Restoration of Long Bone Defect in RabbitsHua ChenXin-Ran JiQun ZhangXue-Zhong TianBo-Xun ZhangPei-Fu TangBackground: The treatment for long bone defects has been a hot topic in the field of regenerative medicine. This study aimed to evaluate the therapeutic effects of calcium sulfate (CS) combined with platelet-rich plasma (PRP) on long bone defect restoration. Methods: A radial bone defect model was constructed through an osteotomy using New Zealand rabbits. The rabbits were randomly divided into four groups (n = 10 in each group): a CS combined with PRP (CS-PRP) group, a CS group, a PRP group, and a positive (recombinant human bone morphogenetic protein-2) control group. PRP was prepared from autologous blood using a two-step centrifugation process. CS-PRP was obtained by mixing hemihydrate CS with PRP. Radiographs and histologic micrographs were generated. The percentage of bone regenerated bone area in each rabbit was calculated at 10 weeks. One-way analysis of variance was performed in this study. Results: The radiographs and histologic micrographs showed bone restoration in the CS-PRP and positive control groups, while nonunion was observed in the CS and PRP groups. The percentages of bone regenerated bone area in the CS-PRP (84.60 ± 2.87%) and positive control (52.21 ± 4.53%) groups were significantly greater than those in the CS group (12.34 ± 2.17%) and PRP group (16.52 ± 4.22%) (P < 0.001). In addition, the bone strength of CS-PRP group (43.10 ± 4.10%) was significantly greater than that of the CS group (20.10 ± 3.70%) or PRP group (25.10 ± 2.10%) (P < 0.001). Conclusion: CS-PRP functions as an effective treatment for long bone defects through stimulating bone regeneration and enhancing new bone strength.http://www.cmj.org/article.asp?issn=0366-6999;year=2016;volume=129;issue=5;spage=557;epage=561;aulast=ChenBone Restoration; Calcium Sulfate; Long Bone Defect; Platelet-rich Plasma |
| spellingShingle | Hua Chen Xin-Ran Ji Qun Zhang Xue-Zhong Tian Bo-Xun Zhang Pei-Fu Tang Effects of Calcium Sulfate Combined with Platelet-rich Plasma on Restoration of Long Bone Defect in Rabbits Chinese Medical Journal Bone Restoration; Calcium Sulfate; Long Bone Defect; Platelet-rich Plasma |
| title | Effects of Calcium Sulfate Combined with Platelet-rich Plasma on Restoration of Long Bone Defect in Rabbits |
| title_full | Effects of Calcium Sulfate Combined with Platelet-rich Plasma on Restoration of Long Bone Defect in Rabbits |
| title_fullStr | Effects of Calcium Sulfate Combined with Platelet-rich Plasma on Restoration of Long Bone Defect in Rabbits |
| title_full_unstemmed | Effects of Calcium Sulfate Combined with Platelet-rich Plasma on Restoration of Long Bone Defect in Rabbits |
| title_short | Effects of Calcium Sulfate Combined with Platelet-rich Plasma on Restoration of Long Bone Defect in Rabbits |
| title_sort | effects of calcium sulfate combined with platelet rich plasma on restoration of long bone defect in rabbits |
| topic | Bone Restoration; Calcium Sulfate; Long Bone Defect; Platelet-rich Plasma |
| url | http://www.cmj.org/article.asp?issn=0366-6999;year=2016;volume=129;issue=5;spage=557;epage=561;aulast=Chen |
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