Bacteriological profile of anaerobes in deep-seated abscess of patients attending a tertiary care hospital
Background: Anaerobes are recognized as important human pathogens causing severe life threatening exogenous and endogenous infection if left untreated. Anaerobes are one of the most neglected pathogens in various clinical samples due to the problem in sample handling, technical difficulties in their...
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| Format: | Article |
| Language: | English |
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Manipal College of Medical Sciences, Pokhara
2022-08-01
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| Series: | Asian Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://www.nepjol.info/index.php/AJMS/article/view/44322 |
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| author | Retina Paul Nabamita Chaudhury Saswati Chattopadhyay Rabindra Nath Misra Sankha Subhra Chaudhury |
| author_facet | Retina Paul Nabamita Chaudhury Saswati Chattopadhyay Rabindra Nath Misra Sankha Subhra Chaudhury |
| author_sort | Retina Paul |
| collection | DOAJ |
| description | Background: Anaerobes are recognized as important human pathogens causing severe life threatening exogenous and endogenous infection if left untreated. Anaerobes are one of the most neglected pathogens in various clinical samples due to the problem in sample handling, technical difficulties in their cultivation, and identification along with prolonged turnaround time.
Aims and Objectives: The present study was undertaken to identify the different anaerobic organisms associated with deep seated abscess and their association with various risk.
Materials and Methods: Pus and fluid sample collected in sterile syringe or swab stick were immediately put in RCM and taken to the laboratory. Gram staining, ZN stain, and culture--both aerobic and anaerobic were done. Obligate anaerobes were checked for aerotolerance. Subcultures were done for identification of species level by Gram stain, colony morphology, biochemical tests, and final identification that were done by the Vitek 2 system.
Results: Out of the 170 samples, 144 (84.70%) were culture positive and the rest 26 (15.29%) were culture negative; 101 (70.1%) were aerobic, 23 (16%) anaerobes, and 20 (13.9%) mixed aerobic and anaerobic. A total of 51 obligate anaerobes were isolated from various samples. Out of which 32 (62.74 %) anaerobic Gram-positive cocci-Peptostreptococcus anaerobious being the most common and 13 (25.49%) anaerobic Gram-negative bacilli --Bacteroids fragilis being most common and 6 (11.76%) were anaerobic Gram-positive bacilli - Actinomyces meyeri being the most common. Diabetes mellitus was a significant associated factor. Maximum number of anaerobes was isolated from abscess over oral cavity followed by gangrenous foot, scrotal abscess, and diabetic foot.
Conclusion: Anaerobes are an important cause of deep-seated abscess—mostly being polymicrobial in nature. Incision--drainage and proper antibiotic therapy is necessary for their early control and prevention of complications. |
| first_indexed | 2024-12-11T20:18:23Z |
| format | Article |
| id | doaj.art-5af0b62dfb2b4d8daa7f2b75c3889c0a |
| institution | Directory Open Access Journal |
| issn | 2467-9100 2091-0576 |
| language | English |
| last_indexed | 2024-12-11T20:18:23Z |
| publishDate | 2022-08-01 |
| publisher | Manipal College of Medical Sciences, Pokhara |
| record_format | Article |
| series | Asian Journal of Medical Sciences |
| spelling | doaj.art-5af0b62dfb2b4d8daa7f2b75c3889c0a2022-12-22T00:52:08ZengManipal College of Medical Sciences, PokharaAsian Journal of Medical Sciences2467-91002091-05762022-08-01138149153https://doi.org/10.3126/ajms.v13i8.44322Bacteriological profile of anaerobes in deep-seated abscess of patients attending a tertiary care hospitalRetina Paul 0https://orcid.org/0000-0002-2205-6596Nabamita Chaudhury 1https://orcid.org/0000-0001-7929-6294Saswati Chattopadhyay 2https://orcid.org/0000-0002-0945-4637Rabindra Nath Misra 3https://orcid.org/0000-0003-0666-0926Sankha Subhra Chaudhury 4https://orcid.org/0000-0002-4062-6214Associate Professor, Department of Microbiology, College of Medicine and JNM Hospital, Kalyani, West Bengal, India Assistant Professor, Department of Microbiology, Burdwan Medical College, Burdwan, West Bengal, India Associate Professor, Department of Microbiology, Burdwan Medical College, Burdwan, West Bengal, India Ex-Professor and Head, Department of Microbiology, Dr. D.Y. Patil Medical College, Pune, Maharashtra, India Senior Resident, Department of Ophthalmology, Jalpaiguri Medical College, Jalpaiguri, West Bengal, India Background: Anaerobes are recognized as important human pathogens causing severe life threatening exogenous and endogenous infection if left untreated. Anaerobes are one of the most neglected pathogens in various clinical samples due to the problem in sample handling, technical difficulties in their cultivation, and identification along with prolonged turnaround time. Aims and Objectives: The present study was undertaken to identify the different anaerobic organisms associated with deep seated abscess and their association with various risk. Materials and Methods: Pus and fluid sample collected in sterile syringe or swab stick were immediately put in RCM and taken to the laboratory. Gram staining, ZN stain, and culture--both aerobic and anaerobic were done. Obligate anaerobes were checked for aerotolerance. Subcultures were done for identification of species level by Gram stain, colony morphology, biochemical tests, and final identification that were done by the Vitek 2 system. Results: Out of the 170 samples, 144 (84.70%) were culture positive and the rest 26 (15.29%) were culture negative; 101 (70.1%) were aerobic, 23 (16%) anaerobes, and 20 (13.9%) mixed aerobic and anaerobic. A total of 51 obligate anaerobes were isolated from various samples. Out of which 32 (62.74 %) anaerobic Gram-positive cocci-Peptostreptococcus anaerobious being the most common and 13 (25.49%) anaerobic Gram-negative bacilli --Bacteroids fragilis being most common and 6 (11.76%) were anaerobic Gram-positive bacilli - Actinomyces meyeri being the most common. Diabetes mellitus was a significant associated factor. Maximum number of anaerobes was isolated from abscess over oral cavity followed by gangrenous foot, scrotal abscess, and diabetic foot. Conclusion: Anaerobes are an important cause of deep-seated abscess—mostly being polymicrobial in nature. Incision--drainage and proper antibiotic therapy is necessary for their early control and prevention of complications.https://www.nepjol.info/index.php/AJMS/article/view/44322anaerobic bacteria; deep-seated abscess; anaerobic gram positive bacilli; anaerobic gram negative bacilli |
| spellingShingle | Retina Paul Nabamita Chaudhury Saswati Chattopadhyay Rabindra Nath Misra Sankha Subhra Chaudhury Bacteriological profile of anaerobes in deep-seated abscess of patients attending a tertiary care hospital Asian Journal of Medical Sciences anaerobic bacteria; deep-seated abscess; anaerobic gram positive bacilli; anaerobic gram negative bacilli |
| title | Bacteriological profile of anaerobes in deep-seated abscess of patients attending a tertiary care hospital |
| title_full | Bacteriological profile of anaerobes in deep-seated abscess of patients attending a tertiary care hospital |
| title_fullStr | Bacteriological profile of anaerobes in deep-seated abscess of patients attending a tertiary care hospital |
| title_full_unstemmed | Bacteriological profile of anaerobes in deep-seated abscess of patients attending a tertiary care hospital |
| title_short | Bacteriological profile of anaerobes in deep-seated abscess of patients attending a tertiary care hospital |
| title_sort | bacteriological profile of anaerobes in deep seated abscess of patients attending a tertiary care hospital |
| topic | anaerobic bacteria; deep-seated abscess; anaerobic gram positive bacilli; anaerobic gram negative bacilli |
| url | https://www.nepjol.info/index.php/AJMS/article/view/44322 |
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