A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism
Erythritol is a natural sweetener produced by microorganisms as an osmoprotectant. It belongs to the group of polyols and it can be utilized by the oleaginous yeast Yarrowia lipolytica. Despite the recent identification of the transcription factor of erythritol utilization (EUF1), the metabolic path...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-05-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01122/full |
| _version_ | 1818016454946062336 |
|---|---|
| author | Aleksandra M. Mirończuk Anna Biegalska Karolina Zugaj Dorota A. Rzechonek Adam Dobrowolski |
| author_facet | Aleksandra M. Mirończuk Anna Biegalska Karolina Zugaj Dorota A. Rzechonek Adam Dobrowolski |
| author_sort | Aleksandra M. Mirończuk |
| collection | DOAJ |
| description | Erythritol is a natural sweetener produced by microorganisms as an osmoprotectant. It belongs to the group of polyols and it can be utilized by the oleaginous yeast Yarrowia lipolytica. Despite the recent identification of the transcription factor of erythritol utilization (EUF1), the metabolic pathway of erythritol catabolism remains unknown. In this study we identified a new gene, YALI0F01628g, involved in erythritol assimilation. In silico analysis showed that YALI0F01628g is a putative isomerase and it is localized in the same region as EUF1. qRT-PCR analysis of Y. lipolytica showed a significant increase in YALI0F01628g expression during growth on erythritol and after overexpression of EUF1. Moreover, the deletion strain ΔF01628 showed significantly impaired erythritol assimilation, whereas synthesis of erythritol remained unchanged. The results showed that YALI0F1628g is involved in erythritol assimilation; thus we named the gene EYI1. Moreover, we suggest the metabolic pathway of erythritol assimilation in yeast Y. lipolytica. |
| first_indexed | 2024-04-14T07:13:27Z |
| format | Article |
| id | doaj.art-5af0c74f1fed4056b00759529309a124 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-14T07:13:27Z |
| publishDate | 2018-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5af0c74f1fed4056b00759529309a1242022-12-22T02:06:22ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-05-01910.3389/fmicb.2018.01122350340A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol CatabolismAleksandra M. MirończukAnna BiegalskaKarolina ZugajDorota A. RzechonekAdam DobrowolskiErythritol is a natural sweetener produced by microorganisms as an osmoprotectant. It belongs to the group of polyols and it can be utilized by the oleaginous yeast Yarrowia lipolytica. Despite the recent identification of the transcription factor of erythritol utilization (EUF1), the metabolic pathway of erythritol catabolism remains unknown. In this study we identified a new gene, YALI0F01628g, involved in erythritol assimilation. In silico analysis showed that YALI0F01628g is a putative isomerase and it is localized in the same region as EUF1. qRT-PCR analysis of Y. lipolytica showed a significant increase in YALI0F01628g expression during growth on erythritol and after overexpression of EUF1. Moreover, the deletion strain ΔF01628 showed significantly impaired erythritol assimilation, whereas synthesis of erythritol remained unchanged. The results showed that YALI0F1628g is involved in erythritol assimilation; thus we named the gene EYI1. Moreover, we suggest the metabolic pathway of erythritol assimilation in yeast Y. lipolytica.https://www.frontiersin.org/article/10.3389/fmicb.2018.01122/fullerythritolYarrowia lipolyticaerythritol catabolic pathwayisomeraseglycerol |
| spellingShingle | Aleksandra M. Mirończuk Anna Biegalska Karolina Zugaj Dorota A. Rzechonek Adam Dobrowolski A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism Frontiers in Microbiology erythritol Yarrowia lipolytica erythritol catabolic pathway isomerase glycerol |
| title | A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism |
| title_full | A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism |
| title_fullStr | A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism |
| title_full_unstemmed | A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism |
| title_short | A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism |
| title_sort | role of a newly identified isomerase from yarrowia lipolytica in erythritol catabolism |
| topic | erythritol Yarrowia lipolytica erythritol catabolic pathway isomerase glycerol |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.01122/full |
| work_keys_str_mv | AT aleksandrammironczuk aroleofanewlyidentifiedisomerasefromyarrowialipolyticainerythritolcatabolism AT annabiegalska aroleofanewlyidentifiedisomerasefromyarrowialipolyticainerythritolcatabolism AT karolinazugaj aroleofanewlyidentifiedisomerasefromyarrowialipolyticainerythritolcatabolism AT dorotaarzechonek aroleofanewlyidentifiedisomerasefromyarrowialipolyticainerythritolcatabolism AT adamdobrowolski aroleofanewlyidentifiedisomerasefromyarrowialipolyticainerythritolcatabolism AT aleksandrammironczuk roleofanewlyidentifiedisomerasefromyarrowialipolyticainerythritolcatabolism AT annabiegalska roleofanewlyidentifiedisomerasefromyarrowialipolyticainerythritolcatabolism AT karolinazugaj roleofanewlyidentifiedisomerasefromyarrowialipolyticainerythritolcatabolism AT dorotaarzechonek roleofanewlyidentifiedisomerasefromyarrowialipolyticainerythritolcatabolism AT adamdobrowolski roleofanewlyidentifiedisomerasefromyarrowialipolyticainerythritolcatabolism |