STAT3 Inhibitory Activities of Lignans Isolated from the Stems of <i>Lindera obtusiloba</i> Blume
<i>Lindera obtusiloba</i> Blume has several activities, such as anti-inflammatory, anti-allergic, anti-tumor, anti-wrinkle, and antioxidant activities. Interleukin-6 (IL-6) is a classic pro-inflammatory cytokine that is associated with various functions, such as proliferation, invasion,...
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MDPI AG
2023-12-01
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| Series: | Scientia Pharmaceutica |
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| Online Access: | https://www.mdpi.com/2218-0532/91/4/56 |
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| author | Eun-Jae Park Hee Ju Lim Hyung Jin Lim Bong-Sik Yun Soyoung Lee Seung-Jae Lee Seung Woong Lee |
| author_facet | Eun-Jae Park Hee Ju Lim Hyung Jin Lim Bong-Sik Yun Soyoung Lee Seung-Jae Lee Seung Woong Lee |
| author_sort | Eun-Jae Park |
| collection | DOAJ |
| description | <i>Lindera obtusiloba</i> Blume has several activities, such as anti-inflammatory, anti-allergic, anti-tumor, anti-wrinkle, and antioxidant activities. Interleukin-6 (IL-6) is a classic pro-inflammatory cytokine that is associated with various functions, such as proliferation, invasion, inflammatory responses and functions within antioxidant defense systems. In this study, we investigated IL-6-induced STAT3 activation of lignan compounds isolated from <i>L. obtusiloba</i>. The structures of the isolated compounds were elucidated via spectroscopic nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESI-MS). As a result, seven lignans were identified from <i>L. obtusiloba</i>. All the isolated compounds (<b>1</b>–<b>7</b>) were evaluated for their IL-6-induced STAT3 inhibitory effects in Hep3B cells using a luciferase reporter assay. Of the isolates, compounds <b>1</b> and <b>5</b> showed inhibitory effects against IL-6-stimulated STAT3 activation. Furthermore, the mRNA expression levels of inflammation-related genes such as CRP, IL-1b, and SOCS3 were significantly reduced by exposure to compounds <b>1</b> and <b>5</b>. The protein levels of p-STAT3 and p-JAK2 in IL-6-induced U266 cells were regulated in the presence of lignans derived from <i>Lindera obtusiloba</i> by Western blot assay. Based on the results, this study of <i>L. obtusiloba</i> demonstrates that the species has promise as a bioactive candidate for the treatment of IL-6-induced STAT3-related disease. |
| first_indexed | 2024-03-08T20:23:00Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 0036-8709 2218-0532 |
| language | English |
| last_indexed | 2024-03-08T20:23:00Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Scientia Pharmaceutica |
| spelling | doaj.art-5af1a56e547a4984bdc35d80cdabccf32023-12-22T14:39:54ZengMDPI AGScientia Pharmaceutica0036-87092218-05322023-12-019145610.3390/scipharm91040056STAT3 Inhibitory Activities of Lignans Isolated from the Stems of <i>Lindera obtusiloba</i> BlumeEun-Jae Park0Hee Ju Lim1Hyung Jin Lim2Bong-Sik Yun3Soyoung Lee4Seung-Jae Lee5Seung Woong Lee6Functionality Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 56212, Jeonbuk, Republic of KoreaFunctionality Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 56212, Jeonbuk, Republic of KoreaFunctionality Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 56212, Jeonbuk, Republic of KoreaDivision of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan-si 54596, Jeonbuk, Republic of KoreaFunctionality Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 56212, Jeonbuk, Republic of KoreaFunctionality Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 56212, Jeonbuk, Republic of KoreaFunctionality Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 56212, Jeonbuk, Republic of Korea<i>Lindera obtusiloba</i> Blume has several activities, such as anti-inflammatory, anti-allergic, anti-tumor, anti-wrinkle, and antioxidant activities. Interleukin-6 (IL-6) is a classic pro-inflammatory cytokine that is associated with various functions, such as proliferation, invasion, inflammatory responses and functions within antioxidant defense systems. In this study, we investigated IL-6-induced STAT3 activation of lignan compounds isolated from <i>L. obtusiloba</i>. The structures of the isolated compounds were elucidated via spectroscopic nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESI-MS). As a result, seven lignans were identified from <i>L. obtusiloba</i>. All the isolated compounds (<b>1</b>–<b>7</b>) were evaluated for their IL-6-induced STAT3 inhibitory effects in Hep3B cells using a luciferase reporter assay. Of the isolates, compounds <b>1</b> and <b>5</b> showed inhibitory effects against IL-6-stimulated STAT3 activation. Furthermore, the mRNA expression levels of inflammation-related genes such as CRP, IL-1b, and SOCS3 were significantly reduced by exposure to compounds <b>1</b> and <b>5</b>. The protein levels of p-STAT3 and p-JAK2 in IL-6-induced U266 cells were regulated in the presence of lignans derived from <i>Lindera obtusiloba</i> by Western blot assay. Based on the results, this study of <i>L. obtusiloba</i> demonstrates that the species has promise as a bioactive candidate for the treatment of IL-6-induced STAT3-related disease.https://www.mdpi.com/2218-0532/91/4/56<i>Lindera obtusiloba</i> BlumeLignanIL-6JAK2STAT3 |
| spellingShingle | Eun-Jae Park Hee Ju Lim Hyung Jin Lim Bong-Sik Yun Soyoung Lee Seung-Jae Lee Seung Woong Lee STAT3 Inhibitory Activities of Lignans Isolated from the Stems of <i>Lindera obtusiloba</i> Blume Scientia Pharmaceutica <i>Lindera obtusiloba</i> Blume Lignan IL-6 JAK2 STAT3 |
| title | STAT3 Inhibitory Activities of Lignans Isolated from the Stems of <i>Lindera obtusiloba</i> Blume |
| title_full | STAT3 Inhibitory Activities of Lignans Isolated from the Stems of <i>Lindera obtusiloba</i> Blume |
| title_fullStr | STAT3 Inhibitory Activities of Lignans Isolated from the Stems of <i>Lindera obtusiloba</i> Blume |
| title_full_unstemmed | STAT3 Inhibitory Activities of Lignans Isolated from the Stems of <i>Lindera obtusiloba</i> Blume |
| title_short | STAT3 Inhibitory Activities of Lignans Isolated from the Stems of <i>Lindera obtusiloba</i> Blume |
| title_sort | stat3 inhibitory activities of lignans isolated from the stems of i lindera obtusiloba i blume |
| topic | <i>Lindera obtusiloba</i> Blume Lignan IL-6 JAK2 STAT3 |
| url | https://www.mdpi.com/2218-0532/91/4/56 |
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