Improved Method for the Detection of Highly Polar Pesticides and Their Main Metabolites in Foods of Animal Origin: Method Validation and Application to Monitoring Programme
The application of polar pesticides in agricultural production has been of great interest due to their low costs and their high effectiveness. For this reason, the possibility of their transfer to foods of animal origin is of great concern for human health. The manuscript describes the implementatio...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Separations |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2297-8739/10/1/44 |
| _version_ | 1827621984761020416 |
|---|---|
| author | Emanuela Verdini Veronica Maria Teresa Lattanzio Biancamaria Ciasca Laura Fioroni Ivan Pecorelli |
| author_facet | Emanuela Verdini Veronica Maria Teresa Lattanzio Biancamaria Ciasca Laura Fioroni Ivan Pecorelli |
| author_sort | Emanuela Verdini |
| collection | DOAJ |
| description | The application of polar pesticides in agricultural production has been of great interest due to their low costs and their high effectiveness. For this reason, the possibility of their transfer to foods of animal origin is of great concern for human health. The manuscript describes the implementation and validation of an analytical method to detect polar pesticides, at regulatory levels, in three foods of animal origin, including bovine fat, chicken eggs, and cow milk. The method was fully validated to detect glyphosate, glufosinate, and their respective metabolites in the above-mentioned foods obtaining fit-for-purpose sensitivity, recoveries (76–119%), repeatability (≤20%), within-laboratory reproducibility (≤20%), and experimental measurement uncertainty less than 50% as required by the SANTE/11312/2021 criteria. Given the satisfactory results, the applicability of the method to additional molecules belonging to the same category (AMPA, cyanuric acid, ethephon, fosetyl aluminum, HEPA, maleic hydrazide, and N-acetyl-glyphosate) was also evaluated in order to meet possible future requests. Finally, the implemented method was applied to analyse samples over the period of March 2021 to August 2022 from two Italian regions (Umbria and Marche) within the national monitoring programme. In agreement with previously available data, none of the samples analysed showed the presence of glyphosate and glufosinate at levels above the legal limit. |
| first_indexed | 2024-03-09T11:14:53Z |
| format | Article |
| id | doaj.art-5af3211e124144b5a5b3b315141bbe98 |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-09T11:14:53Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-5af3211e124144b5a5b3b315141bbe982023-12-01T00:32:43ZengMDPI AGSeparations2297-87392023-01-011014410.3390/separations10010044Improved Method for the Detection of Highly Polar Pesticides and Their Main Metabolites in Foods of Animal Origin: Method Validation and Application to Monitoring ProgrammeEmanuela Verdini0Veronica Maria Teresa Lattanzio1Biancamaria Ciasca2Laura Fioroni3Ivan Pecorelli4Chemistry Department, Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”, 06126 Perugia, ItalyNational Research Council of Italy, Institute of Sciences of Food Production, 70126 Bari, ItalyNational Research Council of Italy, Institute of Sciences of Food Production, 70126 Bari, ItalyChemistry Department, Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”, 06126 Perugia, ItalyChemistry Department, Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”, 06126 Perugia, ItalyThe application of polar pesticides in agricultural production has been of great interest due to their low costs and their high effectiveness. For this reason, the possibility of their transfer to foods of animal origin is of great concern for human health. The manuscript describes the implementation and validation of an analytical method to detect polar pesticides, at regulatory levels, in three foods of animal origin, including bovine fat, chicken eggs, and cow milk. The method was fully validated to detect glyphosate, glufosinate, and their respective metabolites in the above-mentioned foods obtaining fit-for-purpose sensitivity, recoveries (76–119%), repeatability (≤20%), within-laboratory reproducibility (≤20%), and experimental measurement uncertainty less than 50% as required by the SANTE/11312/2021 criteria. Given the satisfactory results, the applicability of the method to additional molecules belonging to the same category (AMPA, cyanuric acid, ethephon, fosetyl aluminum, HEPA, maleic hydrazide, and N-acetyl-glyphosate) was also evaluated in order to meet possible future requests. Finally, the implemented method was applied to analyse samples over the period of March 2021 to August 2022 from two Italian regions (Umbria and Marche) within the national monitoring programme. In agreement with previously available data, none of the samples analysed showed the presence of glyphosate and glufosinate at levels above the legal limit.https://www.mdpi.com/2297-8739/10/1/44glyphosatepolar pesticidescow milkchicken eggbovine fatmultiannual control programmes for pesticide residues |
| spellingShingle | Emanuela Verdini Veronica Maria Teresa Lattanzio Biancamaria Ciasca Laura Fioroni Ivan Pecorelli Improved Method for the Detection of Highly Polar Pesticides and Their Main Metabolites in Foods of Animal Origin: Method Validation and Application to Monitoring Programme Separations glyphosate polar pesticides cow milk chicken egg bovine fat multiannual control programmes for pesticide residues |
| title | Improved Method for the Detection of Highly Polar Pesticides and Their Main Metabolites in Foods of Animal Origin: Method Validation and Application to Monitoring Programme |
| title_full | Improved Method for the Detection of Highly Polar Pesticides and Their Main Metabolites in Foods of Animal Origin: Method Validation and Application to Monitoring Programme |
| title_fullStr | Improved Method for the Detection of Highly Polar Pesticides and Their Main Metabolites in Foods of Animal Origin: Method Validation and Application to Monitoring Programme |
| title_full_unstemmed | Improved Method for the Detection of Highly Polar Pesticides and Their Main Metabolites in Foods of Animal Origin: Method Validation and Application to Monitoring Programme |
| title_short | Improved Method for the Detection of Highly Polar Pesticides and Their Main Metabolites in Foods of Animal Origin: Method Validation and Application to Monitoring Programme |
| title_sort | improved method for the detection of highly polar pesticides and their main metabolites in foods of animal origin method validation and application to monitoring programme |
| topic | glyphosate polar pesticides cow milk chicken egg bovine fat multiannual control programmes for pesticide residues |
| url | https://www.mdpi.com/2297-8739/10/1/44 |
| work_keys_str_mv | AT emanuelaverdini improvedmethodforthedetectionofhighlypolarpesticidesandtheirmainmetabolitesinfoodsofanimaloriginmethodvalidationandapplicationtomonitoringprogramme AT veronicamariateresalattanzio improvedmethodforthedetectionofhighlypolarpesticidesandtheirmainmetabolitesinfoodsofanimaloriginmethodvalidationandapplicationtomonitoringprogramme AT biancamariaciasca improvedmethodforthedetectionofhighlypolarpesticidesandtheirmainmetabolitesinfoodsofanimaloriginmethodvalidationandapplicationtomonitoringprogramme AT laurafioroni improvedmethodforthedetectionofhighlypolarpesticidesandtheirmainmetabolitesinfoodsofanimaloriginmethodvalidationandapplicationtomonitoringprogramme AT ivanpecorelli improvedmethodforthedetectionofhighlypolarpesticidesandtheirmainmetabolitesinfoodsofanimaloriginmethodvalidationandapplicationtomonitoringprogramme |