Phosphorylation of CAD1, PLDdelta, NDT1, RPM1 Proteins Induce Resistance in Tomatoes Infected by <i>Ralstonia solanacearum</i>
<i>Ralstonia solanacaerum</i> is one of the most devastating bacteria causing bacterial wilt disease in more than 200 species of plants, especially those belonging to the family <i>Solanaceae</i>. To cope with this pathogen, plants have evolved different resistance mechanisms...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/6/726 |
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| author | Prachumporn Nounurai Anis Afifah Suthathip Kittisenachai Sittiruk Roytrakul |
| author_facet | Prachumporn Nounurai Anis Afifah Suthathip Kittisenachai Sittiruk Roytrakul |
| author_sort | Prachumporn Nounurai |
| collection | DOAJ |
| description | <i>Ralstonia solanacaerum</i> is one of the most devastating bacteria causing bacterial wilt disease in more than 200 species of plants, especially those belonging to the family <i>Solanaceae</i>. To cope with this pathogen, plants have evolved different resistance mechanisms depending on signal transduction after perception. Phosphorylation is the central regulatory component of the signal transduction pathway. We investigated a comparative phosphoproteomics analysis of the stems of resistant and susceptible tomatoes at 15 min and 30 min after inoculation with <i>Ralstonia solanacearum</i> to determine the phosphorylated proteins involved in induced resistance. Phosphoprotein profiling analyses led to the identification of 969 phosphoproteins classified into 10 functional categories. Among these, six phosphoproteins were uniquely identified in resistant plants including cinnamyl alcohol dehydrogenase 1 (CAD1), mitogen-activated protein kinase kinase kinase 18 (MAPKKK18), phospholipase D delta (PLDDELTA), nicotinamide adenine dinucleotide transporter 1 (NDT1), B3 domain-containing transcription factor VRN1, and disease resistance protein RPM1 (RPM1). These proteins are typically involved in defense mechanisms across different plant species. qRT-PCR analyses were performed to evaluate the level of expression of these genes in resistant and susceptible tomatoes. This study provides useful data, leading to an understanding of the early defense mechanisms of tomatoes against <i>R. solanacearum</i>. |
| first_indexed | 2024-03-09T12:55:53Z |
| format | Article |
| id | doaj.art-5af3c34c19f74bdc9f2e18d6018f6d8c |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T12:55:53Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-5af3c34c19f74bdc9f2e18d6018f6d8c2023-11-30T22:00:13ZengMDPI AGPlants2223-77472022-03-0111672610.3390/plants11060726Phosphorylation of CAD1, PLDdelta, NDT1, RPM1 Proteins Induce Resistance in Tomatoes Infected by <i>Ralstonia solanacearum</i>Prachumporn Nounurai0Anis Afifah1Suthathip Kittisenachai2Sittiruk Roytrakul3Innovative Plant Biotechnology and Precision Agriculture Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, ThailandMolecular and Applied Microbiology Laboratory, Diponegoro University, Jawa Tengah 50275, IndonesiaFunctional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, ThailandFunctional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, Thailand<i>Ralstonia solanacaerum</i> is one of the most devastating bacteria causing bacterial wilt disease in more than 200 species of plants, especially those belonging to the family <i>Solanaceae</i>. To cope with this pathogen, plants have evolved different resistance mechanisms depending on signal transduction after perception. Phosphorylation is the central regulatory component of the signal transduction pathway. We investigated a comparative phosphoproteomics analysis of the stems of resistant and susceptible tomatoes at 15 min and 30 min after inoculation with <i>Ralstonia solanacearum</i> to determine the phosphorylated proteins involved in induced resistance. Phosphoprotein profiling analyses led to the identification of 969 phosphoproteins classified into 10 functional categories. Among these, six phosphoproteins were uniquely identified in resistant plants including cinnamyl alcohol dehydrogenase 1 (CAD1), mitogen-activated protein kinase kinase kinase 18 (MAPKKK18), phospholipase D delta (PLDDELTA), nicotinamide adenine dinucleotide transporter 1 (NDT1), B3 domain-containing transcription factor VRN1, and disease resistance protein RPM1 (RPM1). These proteins are typically involved in defense mechanisms across different plant species. qRT-PCR analyses were performed to evaluate the level of expression of these genes in resistant and susceptible tomatoes. This study provides useful data, leading to an understanding of the early defense mechanisms of tomatoes against <i>R. solanacearum</i>.https://www.mdpi.com/2223-7747/11/6/726<i>Ralstonia solanacearum</i>tomato<i>Solanum lycopersicon</i>phosphorylationbacterial wiltdefense response |
| spellingShingle | Prachumporn Nounurai Anis Afifah Suthathip Kittisenachai Sittiruk Roytrakul Phosphorylation of CAD1, PLDdelta, NDT1, RPM1 Proteins Induce Resistance in Tomatoes Infected by <i>Ralstonia solanacearum</i> Plants <i>Ralstonia solanacearum</i> tomato <i>Solanum lycopersicon</i> phosphorylation bacterial wilt defense response |
| title | Phosphorylation of CAD1, PLDdelta, NDT1, RPM1 Proteins Induce Resistance in Tomatoes Infected by <i>Ralstonia solanacearum</i> |
| title_full | Phosphorylation of CAD1, PLDdelta, NDT1, RPM1 Proteins Induce Resistance in Tomatoes Infected by <i>Ralstonia solanacearum</i> |
| title_fullStr | Phosphorylation of CAD1, PLDdelta, NDT1, RPM1 Proteins Induce Resistance in Tomatoes Infected by <i>Ralstonia solanacearum</i> |
| title_full_unstemmed | Phosphorylation of CAD1, PLDdelta, NDT1, RPM1 Proteins Induce Resistance in Tomatoes Infected by <i>Ralstonia solanacearum</i> |
| title_short | Phosphorylation of CAD1, PLDdelta, NDT1, RPM1 Proteins Induce Resistance in Tomatoes Infected by <i>Ralstonia solanacearum</i> |
| title_sort | phosphorylation of cad1 plddelta ndt1 rpm1 proteins induce resistance in tomatoes infected by i ralstonia solanacearum i |
| topic | <i>Ralstonia solanacearum</i> tomato <i>Solanum lycopersicon</i> phosphorylation bacterial wilt defense response |
| url | https://www.mdpi.com/2223-7747/11/6/726 |
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