Uterine metabolic disorder induced by silica nanoparticles: biodistribution and bioactivity revealed by labeling with FITC
Abstract Extensive application of nanomaterials has dramatically increased the risk of silica nanoparticle (SiNP, SiO2) exposure, yet their biological effect on reproduction has not been fully elucidated. By tracking the uterine biodistribution of SiNP in pregnant mice, this study was conducted to e...
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| Format: | Article |
| Language: | English |
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BMC
2021-02-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-021-00810-x |
| _version_ | 1811186949318246400 |
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| author | Shuyin Duan Meihua Zhang Junxia Li Jiaqi Tian Haoyu Yin Xietong Wang Lin Zhang |
| author_facet | Shuyin Duan Meihua Zhang Junxia Li Jiaqi Tian Haoyu Yin Xietong Wang Lin Zhang |
| author_sort | Shuyin Duan |
| collection | DOAJ |
| description | Abstract Extensive application of nanomaterials has dramatically increased the risk of silica nanoparticle (SiNP, SiO2) exposure, yet their biological effect on reproduction has not been fully elucidated. By tracking the uterine biodistribution of SiNP in pregnant mice, this study was conducted to evaluate the biological effect of SiNP on reproduction. First, SiNP was conjugated with FITC, and then the FITC-SiNP was administrated to trophoblast (100 µg/mL, 24 h) in vitro and pregnant mice (0.25 mg/mouse, 2–24 h) in vivo. It was found that the FITC-SiNP was internalized by trophoblast and deposited in the uterus. The internalization of SiNP caused trophoblast dysfunction and apoptosis, while SiNP accumulation in the uterus induced diffuse inflammatory infiltration. The genome-wide alteration of gene expression was studied by high throughput sequencing analysis, where 75 genes were found to be dysregulated after SiNP exposure, among which ACOT2, SCD1, and CPT1A were demonstrated to regulate the biosynthesis of unsaturated fatty acids. Moreover, the suppression of unsaturated fatty acids caused mitochondrial overload of long-chain fatty acyl-CoA (LACoA), which further induced both trophoblast apoptosis and endometrial inflammation. In conclusion, the successful conjugation of FITC onto SiNP facilitated the tracking of SiNP in vitro and in vivo, while exposure to FITC-SiNP induced uterine metabolic disorder, which was regulated by the ACOT/CPT1A/SCD1 axis through the biosynthesis of unsaturated fatty acids signaling pathway. |
| first_indexed | 2024-04-11T13:53:38Z |
| format | Article |
| id | doaj.art-5af82759f45d4fe49cc548dd655085ea |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T13:53:38Z |
| publishDate | 2021-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-5af82759f45d4fe49cc548dd655085ea2022-12-22T04:20:25ZengBMCJournal of Nanobiotechnology1477-31552021-02-0119111410.1186/s12951-021-00810-xUterine metabolic disorder induced by silica nanoparticles: biodistribution and bioactivity revealed by labeling with FITCShuyin Duan0Meihua Zhang1Junxia Li2Jiaqi Tian3Haoyu Yin4Xietong Wang5Lin Zhang6Key Laboratory of Birth Regulation and Control Technology of National Health Commission of China, Maternal and Child Health Care Hospital of Shandong Province, Shandong UniversityKey Laboratory of Birth Regulation and Control Technology of National Health Commission of China, Maternal and Child Health Care Hospital of Shandong Province, Shandong UniversitySchool of Public Health, Weifang Medical UniversitySchool of Public Health, Weifang Medical UniversitySchool of Public Health, Weifang Medical UniversityKey Laboratory of Birth Regulation and Control Technology of National Health Commission of China, Maternal and Child Health Care Hospital of Shandong Province, Shandong UniversityKey Laboratory of Birth Regulation and Control Technology of National Health Commission of China, Maternal and Child Health Care Hospital of Shandong Province, Shandong UniversityAbstract Extensive application of nanomaterials has dramatically increased the risk of silica nanoparticle (SiNP, SiO2) exposure, yet their biological effect on reproduction has not been fully elucidated. By tracking the uterine biodistribution of SiNP in pregnant mice, this study was conducted to evaluate the biological effect of SiNP on reproduction. First, SiNP was conjugated with FITC, and then the FITC-SiNP was administrated to trophoblast (100 µg/mL, 24 h) in vitro and pregnant mice (0.25 mg/mouse, 2–24 h) in vivo. It was found that the FITC-SiNP was internalized by trophoblast and deposited in the uterus. The internalization of SiNP caused trophoblast dysfunction and apoptosis, while SiNP accumulation in the uterus induced diffuse inflammatory infiltration. The genome-wide alteration of gene expression was studied by high throughput sequencing analysis, where 75 genes were found to be dysregulated after SiNP exposure, among which ACOT2, SCD1, and CPT1A were demonstrated to regulate the biosynthesis of unsaturated fatty acids. Moreover, the suppression of unsaturated fatty acids caused mitochondrial overload of long-chain fatty acyl-CoA (LACoA), which further induced both trophoblast apoptosis and endometrial inflammation. In conclusion, the successful conjugation of FITC onto SiNP facilitated the tracking of SiNP in vitro and in vivo, while exposure to FITC-SiNP induced uterine metabolic disorder, which was regulated by the ACOT/CPT1A/SCD1 axis through the biosynthesis of unsaturated fatty acids signaling pathway.https://doi.org/10.1186/s12951-021-00810-xSilica nanoparticleBiodistributionUterine inflammationUnsaturated fatty acidsTrophoblast |
| spellingShingle | Shuyin Duan Meihua Zhang Junxia Li Jiaqi Tian Haoyu Yin Xietong Wang Lin Zhang Uterine metabolic disorder induced by silica nanoparticles: biodistribution and bioactivity revealed by labeling with FITC Journal of Nanobiotechnology Silica nanoparticle Biodistribution Uterine inflammation Unsaturated fatty acids Trophoblast |
| title | Uterine metabolic disorder induced by silica nanoparticles: biodistribution and bioactivity revealed by labeling with FITC |
| title_full | Uterine metabolic disorder induced by silica nanoparticles: biodistribution and bioactivity revealed by labeling with FITC |
| title_fullStr | Uterine metabolic disorder induced by silica nanoparticles: biodistribution and bioactivity revealed by labeling with FITC |
| title_full_unstemmed | Uterine metabolic disorder induced by silica nanoparticles: biodistribution and bioactivity revealed by labeling with FITC |
| title_short | Uterine metabolic disorder induced by silica nanoparticles: biodistribution and bioactivity revealed by labeling with FITC |
| title_sort | uterine metabolic disorder induced by silica nanoparticles biodistribution and bioactivity revealed by labeling with fitc |
| topic | Silica nanoparticle Biodistribution Uterine inflammation Unsaturated fatty acids Trophoblast |
| url | https://doi.org/10.1186/s12951-021-00810-x |
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