OIL OXIDATIVE ACTIVITY OF SOME STRAINS OF BACTERIA OF PSEUDOMONAS GENUS
The aim. Study of oil oxidative activity of some strains of bacteria of Pseudomonas genus, selected for use in bioremediation of soil. Methods. Bacteria P. fluorescens ОNU328, P. maltophilia ОNU329, P. cepacia ОNU327 have been cultivated in nutrient medium M-9 contained crude oil with density of 0.8...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Odessa I. I. Mechnikov National University
2013-12-01
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| Series: | Mìkrobìologìâ ì Bìotehnologìâ |
| Subjects: | |
| Online Access: | http://mbt.onu.edu.ua/article/view/48979 |
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| author | Т. В. Гудзенко О. В. Волювач Т. О. Бєляєва I. П. Конуп А. Є. Бухтіяров Г. В. Лісютін I. В. Пузирьова О. Г. Горшкова В. О. Iваниця |
| author_facet | Т. В. Гудзенко О. В. Волювач Т. О. Бєляєва I. П. Конуп А. Є. Бухтіяров Г. В. Лісютін I. В. Пузирьова О. Г. Горшкова В. О. Iваниця |
| author_sort | Т. В. Гудзенко |
| collection | DOAJ |
| description | The aim. Study of oil oxidative activity of some strains of bacteria of Pseudomonas genus, selected for use in bioremediation of soil. Methods. Bacteria P. fluorescens ОNU328, P. maltophilia ОNU329, P. cepacia ОNU327 have been cultivated in nutrient medium M-9 contained crude oil with density of 0.84 g /cm3 with the concentration 500 mg /l. The residual content of oil in 10, 20 and 30 days, was determined by the integral method of infrared spectroscopy. Oil extraction of the samples was performed by using carbon tetrachloride (CCl4). Results. It was determined that the bacteria of genus Pseudomonas selected for use in biotechnology of purification of soil, within a month broke down hydrocarbons of crude oil with initial concentration of 500 mg /dm3 up to 70–80%. Oil oxidative activity of all used strains of microorganisms was high in the first 10 days of the exhibition – the degree of biodegradation of petroleum hydrocarbons reached 62.0–73.0%. Conclusion. Bacteria strains of P. cepacia ОNU327, P. maltophilia ONU329 and P. fluorescens ОNU328 can be recommended for the development of technologies of soil bioremediation from oil and oil products. |
| first_indexed | 2024-12-19T13:38:10Z |
| format | Article |
| id | doaj.art-5af9601c7e8b4d9d866475264591f134 |
| institution | Directory Open Access Journal |
| issn | 2076-0558 2307-4663 |
| language | English |
| last_indexed | 2024-12-19T13:38:10Z |
| publishDate | 2013-12-01 |
| publisher | Odessa I. I. Mechnikov National University |
| record_format | Article |
| series | Mìkrobìologìâ ì Bìotehnologìâ |
| spelling | doaj.art-5af9601c7e8b4d9d866475264591f1342022-12-21T20:19:06ZengOdessa I. I. Mechnikov National UniversityMìkrobìologìâ ì Bìotehnologìâ2076-05582307-46632013-12-0104(24)728010.18524/2307-4663.2013.4(24).4897948979OIL OXIDATIVE ACTIVITY OF SOME STRAINS OF BACTERIA OF PSEUDOMONAS GENUSТ. В. Гудзенко0О. В. Волювач1Т. О. Бєляєва2I. П. Конуп3А. Є. Бухтіяров4Г. В. Лісютін5I. В. Пузирьова6О. Г. Горшкова7В. О. Iваниця8Одеський національний університет імені I.I. МечниковаОдеський національний університет імені I.I. МечниковаОдеський національний університет імені I.I. МечниковаОдеський національний університет імені I.I. МечниковаОдеський національний університет імені I.I. МечниковаОдеський національний університет імені I.I. МечниковаОдеський національний університет імені I.I. МечниковаОдеський національний університет імені I.I. МечниковаОдеський національний університет імені I.I. МечниковаThe aim. Study of oil oxidative activity of some strains of bacteria of Pseudomonas genus, selected for use in bioremediation of soil. Methods. Bacteria P. fluorescens ОNU328, P. maltophilia ОNU329, P. cepacia ОNU327 have been cultivated in nutrient medium M-9 contained crude oil with density of 0.84 g /cm3 with the concentration 500 mg /l. The residual content of oil in 10, 20 and 30 days, was determined by the integral method of infrared spectroscopy. Oil extraction of the samples was performed by using carbon tetrachloride (CCl4). Results. It was determined that the bacteria of genus Pseudomonas selected for use in biotechnology of purification of soil, within a month broke down hydrocarbons of crude oil with initial concentration of 500 mg /dm3 up to 70–80%. Oil oxidative activity of all used strains of microorganisms was high in the first 10 days of the exhibition – the degree of biodegradation of petroleum hydrocarbons reached 62.0–73.0%. Conclusion. Bacteria strains of P. cepacia ОNU327, P. maltophilia ONU329 and P. fluorescens ОNU328 can be recommended for the development of technologies of soil bioremediation from oil and oil products.http://mbt.onu.edu.ua/article/view/48979нафтабактерії роду pseudomonasбіодеструкціянафтоокиснювальна активність |
| spellingShingle | Т. В. Гудзенко О. В. Волювач Т. О. Бєляєва I. П. Конуп А. Є. Бухтіяров Г. В. Лісютін I. В. Пузирьова О. Г. Горшкова В. О. Iваниця OIL OXIDATIVE ACTIVITY OF SOME STRAINS OF BACTERIA OF PSEUDOMONAS GENUS Mìkrobìologìâ ì Bìotehnologìâ нафта бактерії роду pseudomonas біодеструкція нафтоокиснювальна активність |
| title | OIL OXIDATIVE ACTIVITY OF SOME STRAINS OF BACTERIA OF PSEUDOMONAS GENUS |
| title_full | OIL OXIDATIVE ACTIVITY OF SOME STRAINS OF BACTERIA OF PSEUDOMONAS GENUS |
| title_fullStr | OIL OXIDATIVE ACTIVITY OF SOME STRAINS OF BACTERIA OF PSEUDOMONAS GENUS |
| title_full_unstemmed | OIL OXIDATIVE ACTIVITY OF SOME STRAINS OF BACTERIA OF PSEUDOMONAS GENUS |
| title_short | OIL OXIDATIVE ACTIVITY OF SOME STRAINS OF BACTERIA OF PSEUDOMONAS GENUS |
| title_sort | oil oxidative activity of some strains of bacteria of pseudomonas genus |
| topic | нафта бактерії роду pseudomonas біодеструкція нафтоокиснювальна активність |
| url | http://mbt.onu.edu.ua/article/view/48979 |
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