Hematological parameters and morphological characteristics of blood cells in turtle and tortoise species within captivity in Sri Lanka
Objective: This study was conducted to determine hematological reference ranges and morpho¬logical characteristics blood cells in healthy captive Sri Lankan fresh water turtle and tortoise species. Materials and Methods: Following turtle species, namely, Melanochelys trijuga parkeri (n = 06), M. tr...
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| Format: | Article |
| Language: | English |
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Network for the Veterinarians of Bangladesh
2019-09-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=30071 |
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| author | Nadeesha H. N. Dilrukshi Anura P. Jayasooriya Gamika A. Prathapasinghe |
| author_facet | Nadeesha H. N. Dilrukshi Anura P. Jayasooriya Gamika A. Prathapasinghe |
| author_sort | Nadeesha H. N. Dilrukshi |
| collection | DOAJ |
| description | Objective: This study was conducted to determine hematological reference ranges and morpho¬logical characteristics blood cells in healthy captive Sri Lankan fresh water turtle and tortoise species.
Materials and Methods: Following turtle species, namely, Melanochelys trijuga parkeri (n = 06), M. trijuga thermalis (n = 06), and Lissemys punctata punctata (n = 06) and a tortoise species Geochelone elegans (n = 06) were evaluated. Blood smears were stained using LeishmanGram staining protocol. The differential white blood cell counts and morphology were assessed using the standard protocols.
Results: Both red blood cells and their nuclei were irregular in M. trijuga parkeri, whereas M. trijuga thermalis had oval-shaped and the nuclei were irregular in shape. L. punctata punctata and G. elegans had oval-shaped red blood cells and their nuclei were round in shape. In terms of differential counts, heterophils were the most abundant leukocyte type in all the species. Melanochelys trijuga parkeri had the highest heterophils value of 55% and the lowest value of 48.5% was recorded with G. elegans. Lymphocytes count was significantly high in G. elegans. Characteristic morphological features were observed in different leukocytes. In terms of thrombo¬cytes, M. trijuga thermalis and L. punctata punctata had single platelets, whereas M. trijuga park¬eri and G. elegans had platelets as clumps. Thrombocytes are ellipsoidal with centrally located dark-stained nuclei and their cytoplasm is clear.
Conclusion: The findings in this study can be used as the reference values in the assessment of health of the above species and also will be useful in future hematological studies related to these species. [J Adv Vet Anim Res 2019; 6(3.000): 266-271] |
| first_indexed | 2024-12-16T16:25:46Z |
| format | Article |
| id | doaj.art-5afbe9d4c79d434388521473dd46e14a |
| institution | Directory Open Access Journal |
| issn | 2311-7710 |
| language | English |
| last_indexed | 2024-12-16T16:25:46Z |
| publishDate | 2019-09-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-5afbe9d4c79d434388521473dd46e14a2022-12-21T22:24:44ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102019-09-016326627110.5455/javar.2019.f34330071Hematological parameters and morphological characteristics of blood cells in turtle and tortoise species within captivity in Sri LankaNadeesha H. N. Dilrukshi0Anura P. Jayasooriya1Gamika A. Prathapasinghe2Department of Livestock and Avian Sciences, Faculty of Livestock, Fisheries and Nutrition, Wayamba University of Sri Lanka, Makandura, Sri Lanka Department of Basic Veterinary Sciences, School of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, Trinidad and Tobago, West Indies. & Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine Animal Science, University of Peradeniya, Peradeniya, Sri Lanka Department of Livestock and Avian Sciences, Faculty of Livestock, Fisheries and Nutrition, Wayamba University of Sri Lanka, Makandura, Sri Lanka.Objective: This study was conducted to determine hematological reference ranges and morpho¬logical characteristics blood cells in healthy captive Sri Lankan fresh water turtle and tortoise species. Materials and Methods: Following turtle species, namely, Melanochelys trijuga parkeri (n = 06), M. trijuga thermalis (n = 06), and Lissemys punctata punctata (n = 06) and a tortoise species Geochelone elegans (n = 06) were evaluated. Blood smears were stained using LeishmanGram staining protocol. The differential white blood cell counts and morphology were assessed using the standard protocols. Results: Both red blood cells and their nuclei were irregular in M. trijuga parkeri, whereas M. trijuga thermalis had oval-shaped and the nuclei were irregular in shape. L. punctata punctata and G. elegans had oval-shaped red blood cells and their nuclei were round in shape. In terms of differential counts, heterophils were the most abundant leukocyte type in all the species. Melanochelys trijuga parkeri had the highest heterophils value of 55% and the lowest value of 48.5% was recorded with G. elegans. Lymphocytes count was significantly high in G. elegans. Characteristic morphological features were observed in different leukocytes. In terms of thrombo¬cytes, M. trijuga thermalis and L. punctata punctata had single platelets, whereas M. trijuga park¬eri and G. elegans had platelets as clumps. Thrombocytes are ellipsoidal with centrally located dark-stained nuclei and their cytoplasm is clear. Conclusion: The findings in this study can be used as the reference values in the assessment of health of the above species and also will be useful in future hematological studies related to these species. [J Adv Vet Anim Res 2019; 6(3.000): 266-271]http://www.ejmanager.com/fulltextpdf.php?mno=30071Captivity; fresh water; hematology; tortoises; turtles |
| spellingShingle | Nadeesha H. N. Dilrukshi Anura P. Jayasooriya Gamika A. Prathapasinghe Hematological parameters and morphological characteristics of blood cells in turtle and tortoise species within captivity in Sri Lanka Journal of Advanced Veterinary and Animal Research Captivity; fresh water; hematology; tortoises; turtles |
| title | Hematological parameters and morphological characteristics of blood cells in turtle and tortoise species within captivity in Sri Lanka |
| title_full | Hematological parameters and morphological characteristics of blood cells in turtle and tortoise species within captivity in Sri Lanka |
| title_fullStr | Hematological parameters and morphological characteristics of blood cells in turtle and tortoise species within captivity in Sri Lanka |
| title_full_unstemmed | Hematological parameters and morphological characteristics of blood cells in turtle and tortoise species within captivity in Sri Lanka |
| title_short | Hematological parameters and morphological characteristics of blood cells in turtle and tortoise species within captivity in Sri Lanka |
| title_sort | hematological parameters and morphological characteristics of blood cells in turtle and tortoise species within captivity in sri lanka |
| topic | Captivity; fresh water; hematology; tortoises; turtles |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=30071 |
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