Evaluation of Cytotoxicity and Genotoxicity of Micromeria pulegium (Rochel) Benth Extract in Human Lymphocytes and Gr-M Melanoma Cells in vitro
Micromeria pulegium (Rochel) Benth. is an endemic species of Lamiacea family that includes frequently used plants in culinary and folk medicine. As cytotoxic potential of some species of Micromeria genus has been confirmed, this study aimed to test unknown antiproliferative and genotoxic potential...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Sarajevo, Institute for Genetic Engineering and Biotechnology
2018-06-01
|
| Series: | Genetics & Applications |
| Subjects: | |
| Online Access: | https://genapp.ba/editions/index.php/journal/article/view/21 |
| _version_ | 1811299033254199296 |
|---|---|
| author | Anja Haverić Jasmina Čakar Maida Hadžić Sanin Haverić |
| author_facet | Anja Haverić Jasmina Čakar Maida Hadžić Sanin Haverić |
| author_sort | Anja Haverić |
| collection | DOAJ |
| description |
Micromeria pulegium (Rochel) Benth. is an endemic species of Lamiacea family that includes frequently used plants in culinary and folk medicine. As cytotoxic potential of some species of Micromeria genus has been confirmed, this study aimed to test unknown antiproliferative and genotoxic potential of M. pulegium, endemic bh species, aqueous leaf extract in normal (human lymphocytes) and cancer (human melanoma GR-M) cells in order to protect small populations of native M. pulegium populations or promote its controlled micropropagation or cultivation. Cytokinesis-block micronucleus cytome assay was applied for human lymphocyte cultures, while trypan blue exclusion assay was used for evaluation of cytotoxicity in human GR-M melanoma cells. Results demonstrate no genotoxic effects up to concentration of 0.2 mg/ml in human lymphocyte in vitro but significant reduction of cell viability in human GR-M melanoma cell line cultures treated with 0.3 mg/ml of Micromeria extract.
|
| first_indexed | 2024-04-13T06:29:14Z |
| format | Article |
| id | doaj.art-5afd8e2da25441f5af13097aa7556eb4 |
| institution | Directory Open Access Journal |
| issn | 2566-2937 2566-431X |
| language | English |
| last_indexed | 2024-04-13T06:29:14Z |
| publishDate | 2018-06-01 |
| publisher | University of Sarajevo, Institute for Genetic Engineering and Biotechnology |
| record_format | Article |
| series | Genetics & Applications |
| spelling | doaj.art-5afd8e2da25441f5af13097aa7556eb42022-12-22T02:58:14ZengUniversity of Sarajevo, Institute for Genetic Engineering and BiotechnologyGenetics & Applications2566-29372566-431X2018-06-0121Evaluation of Cytotoxicity and Genotoxicity of Micromeria pulegium (Rochel) Benth Extract in Human Lymphocytes and Gr-M Melanoma Cells in vitroAnja Haverić0Jasmina Čakar1Maida Hadžić2Sanin Haverić3University of Sarajevo - Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo - Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo - Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo - Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and Herzegovina Micromeria pulegium (Rochel) Benth. is an endemic species of Lamiacea family that includes frequently used plants in culinary and folk medicine. As cytotoxic potential of some species of Micromeria genus has been confirmed, this study aimed to test unknown antiproliferative and genotoxic potential of M. pulegium, endemic bh species, aqueous leaf extract in normal (human lymphocytes) and cancer (human melanoma GR-M) cells in order to protect small populations of native M. pulegium populations or promote its controlled micropropagation or cultivation. Cytokinesis-block micronucleus cytome assay was applied for human lymphocyte cultures, while trypan blue exclusion assay was used for evaluation of cytotoxicity in human GR-M melanoma cells. Results demonstrate no genotoxic effects up to concentration of 0.2 mg/ml in human lymphocyte in vitro but significant reduction of cell viability in human GR-M melanoma cell line cultures treated with 0.3 mg/ml of Micromeria extract. https://genapp.ba/editions/index.php/journal/article/view/21plant extractscell viability,micronucleus |
| spellingShingle | Anja Haverić Jasmina Čakar Maida Hadžić Sanin Haverić Evaluation of Cytotoxicity and Genotoxicity of Micromeria pulegium (Rochel) Benth Extract in Human Lymphocytes and Gr-M Melanoma Cells in vitro Genetics & Applications plant extracts cell viability, micronucleus |
| title | Evaluation of Cytotoxicity and Genotoxicity of Micromeria pulegium (Rochel) Benth Extract in Human Lymphocytes and Gr-M Melanoma Cells in vitro |
| title_full | Evaluation of Cytotoxicity and Genotoxicity of Micromeria pulegium (Rochel) Benth Extract in Human Lymphocytes and Gr-M Melanoma Cells in vitro |
| title_fullStr | Evaluation of Cytotoxicity and Genotoxicity of Micromeria pulegium (Rochel) Benth Extract in Human Lymphocytes and Gr-M Melanoma Cells in vitro |
| title_full_unstemmed | Evaluation of Cytotoxicity and Genotoxicity of Micromeria pulegium (Rochel) Benth Extract in Human Lymphocytes and Gr-M Melanoma Cells in vitro |
| title_short | Evaluation of Cytotoxicity and Genotoxicity of Micromeria pulegium (Rochel) Benth Extract in Human Lymphocytes and Gr-M Melanoma Cells in vitro |
| title_sort | evaluation of cytotoxicity and genotoxicity of micromeria pulegium rochel benth extract in human lymphocytes and gr m melanoma cells in vitro |
| topic | plant extracts cell viability, micronucleus |
| url | https://genapp.ba/editions/index.php/journal/article/view/21 |
| work_keys_str_mv | AT anjahaveric evaluationofcytotoxicityandgenotoxicityofmicromeriapulegiumrochelbenthextractinhumanlymphocytesandgrmmelanomacellsinvitro AT jasminacakar evaluationofcytotoxicityandgenotoxicityofmicromeriapulegiumrochelbenthextractinhumanlymphocytesandgrmmelanomacellsinvitro AT maidahadzic evaluationofcytotoxicityandgenotoxicityofmicromeriapulegiumrochelbenthextractinhumanlymphocytesandgrmmelanomacellsinvitro AT saninhaveric evaluationofcytotoxicityandgenotoxicityofmicromeriapulegiumrochelbenthextractinhumanlymphocytesandgrmmelanomacellsinvitro |