Ultrasound-Assisted Extraction of Phenolics and Antioxidants from Propolis for Stabilization of Sunflower Oil

Propolis is one of the byproducts of honey bees; it contains many phenolic compounds which are some of the most important natural antioxidants. The present study examined the antioxidant activity of propolis and its role in the stability of sunflower oil as a natural antioxidant in the form of Box–B...

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Main Authors: Zeinab Golmahi, Amir Hossein Elhamirad
Format: Article
Language:English
Published: Universidad de Nariño 2021-06-01
Series:Revista de Ciencias Agrícolas
Subjects:
Online Access:https://revistas.udenar.edu.co/index.php/rfacia/article/view/6498
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author Zeinab Golmahi
Amir Hossein Elhamirad
author_facet Zeinab Golmahi
Amir Hossein Elhamirad
author_sort Zeinab Golmahi
collection DOAJ
description Propolis is one of the byproducts of honey bees; it contains many phenolic compounds which are some of the most important natural antioxidants. The present study examined the antioxidant activity of propolis and its role in the stability of sunflower oil as a natural antioxidant in the form of Box–Behnken design. The extraction process was performed using an ultrasonic bath method with two factors in three levels which included 18 assays by the response surface methods. The investigated variables included the concentration of the extract (min: 30 and max: 1000 ppm), the extraction time (min: 10 and max: 30 minutes), and temperature (min: 37.5 and max: 50°C). After analysis of the data in optimal conditions, the concentration of propolis ethanol extract, extraction temperature, and extraction time was determined as 997.5 ppm, 34.6°C, and 30 min, respectively. In this condition, the total phenolic compounds and the free-radical scavenging properties were reported as 253.1mgGAE/100gDW and 85.9%, respectively. The result of the evaluation of the oxidative stability of the optimized sample oil showed reduced peroxide and thiobarbituric acid index compared to the control sample and synthetic antioxidants (BHT); also, the oil stability improved significantly over time.
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spelling doaj.art-5afea047247943ab83ab5361742940b12022-12-21T23:09:04ZengUniversidad de NariñoRevista de Ciencias Agrícolas0120-01352256-22732021-06-01381419https://doi.org/10.22267/rcia.213801.141Ultrasound-Assisted Extraction of Phenolics and Antioxidants from Propolis for Stabilization of Sunflower OilZeinab Golmahi0https://orcid.org/0000-0001-8794-8811Amir Hossein Elhamirad1https://orcid.org/0000-0002-4483-2568Islamic Azad UniversityIslamic Azad UniversityPropolis is one of the byproducts of honey bees; it contains many phenolic compounds which are some of the most important natural antioxidants. The present study examined the antioxidant activity of propolis and its role in the stability of sunflower oil as a natural antioxidant in the form of Box–Behnken design. The extraction process was performed using an ultrasonic bath method with two factors in three levels which included 18 assays by the response surface methods. The investigated variables included the concentration of the extract (min: 30 and max: 1000 ppm), the extraction time (min: 10 and max: 30 minutes), and temperature (min: 37.5 and max: 50°C). After analysis of the data in optimal conditions, the concentration of propolis ethanol extract, extraction temperature, and extraction time was determined as 997.5 ppm, 34.6°C, and 30 min, respectively. In this condition, the total phenolic compounds and the free-radical scavenging properties were reported as 253.1mgGAE/100gDW and 85.9%, respectively. The result of the evaluation of the oxidative stability of the optimized sample oil showed reduced peroxide and thiobarbituric acid index compared to the control sample and synthetic antioxidants (BHT); also, the oil stability improved significantly over time.https://revistas.udenar.edu.co/index.php/rfacia/article/view/6498propolisantioxidant activityultrasonic bath extractiontotal phenolic compoundsoxidative stability
spellingShingle Zeinab Golmahi
Amir Hossein Elhamirad
Ultrasound-Assisted Extraction of Phenolics and Antioxidants from Propolis for Stabilization of Sunflower Oil
Revista de Ciencias Agrícolas
propolis
antioxidant activity
ultrasonic bath extraction
total phenolic compounds
oxidative stability
title Ultrasound-Assisted Extraction of Phenolics and Antioxidants from Propolis for Stabilization of Sunflower Oil
title_full Ultrasound-Assisted Extraction of Phenolics and Antioxidants from Propolis for Stabilization of Sunflower Oil
title_fullStr Ultrasound-Assisted Extraction of Phenolics and Antioxidants from Propolis for Stabilization of Sunflower Oil
title_full_unstemmed Ultrasound-Assisted Extraction of Phenolics and Antioxidants from Propolis for Stabilization of Sunflower Oil
title_short Ultrasound-Assisted Extraction of Phenolics and Antioxidants from Propolis for Stabilization of Sunflower Oil
title_sort ultrasound assisted extraction of phenolics and antioxidants from propolis for stabilization of sunflower oil
topic propolis
antioxidant activity
ultrasonic bath extraction
total phenolic compounds
oxidative stability
url https://revistas.udenar.edu.co/index.php/rfacia/article/view/6498
work_keys_str_mv AT zeinabgolmahi ultrasoundassistedextractionofphenolicsandantioxidantsfrompropolisforstabilizationofsunfloweroil
AT amirhosseinelhamirad ultrasoundassistedextractionofphenolicsandantioxidantsfrompropolisforstabilizationofsunfloweroil