Activation of TF-Dependent Blood Coagulation Pathway and VEGF-A in Patients with Essential Thrombocythemia
<i>Background and objectives:</i> Recent studies suggest that a vascular endothelial growth factor (VEGF-A) may be involved in the thrombotic process by stimulating the expression of tissue factor in vascular endothelial cells. Tissue factor (TF) can also stimulate the transcription of t...
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MDPI AG
2019-02-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1010-660X/55/2/54 |
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| author | Grażyna Gadomska Katarzyna Ziołkowska Joanna Boinska Jan Filipiak Danuta Rość |
| author_facet | Grażyna Gadomska Katarzyna Ziołkowska Joanna Boinska Jan Filipiak Danuta Rość |
| author_sort | Grażyna Gadomska |
| collection | DOAJ |
| description | <i>Background and objectives:</i> Recent studies suggest that a vascular endothelial growth factor (VEGF-A) may be involved in the thrombotic process by stimulating the expression of tissue factor in vascular endothelial cells. Tissue factor (TF) can also stimulate the transcription of the gene encoding VEGF-A. The relationship between coagulation and angiogenesis in myeloproliferative neoplasms is not fully understood. The aim of this study was to evaluate the concentration of TF in relation to VEGF-A in the blood of patients with essential thrombocythemia (ET). <i>Patients and methods:</i> The study group consisted of 130, newly diagnosed patients with ET (mean age 61 years). The control group consisted of 35 healthy volunteers (mean age 51 years). Concentrations of VEGF-A, TF, and tissue factor pathway inhibitor (TFPI) were analysed using immunoenzymatic methods. TF and TFPI activities were performed using chromogenic assays. <i>Results:</i> The median concentration of TF Ag was 3-fold higher and the TF activity was more than 15-fold higher in ET patients than in normal individuals. There were no statistically significant differences in the TFPI concentration and activity between groups. VEGF-A was significantly increased in patients with ET (<i>p</i> < 0.000001). Analysis of correlations revealed a positive correlation between VEGF-A and TF Ag as well as a positive correlation between VEGF-A and TFPI activity. <i>Conclusions:</i> The simultaneous increase of TF concentration and activity, VEGF-A in the blood of patients with ET, as well as a positive correlation between the concentration of TF and VEGF-A demonstrates the coexistence of TF-dependent coagulation and activation of angiogenesis. |
| first_indexed | 2024-03-12T11:16:38Z |
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| last_indexed | 2024-03-12T11:16:38Z |
| publishDate | 2019-02-01 |
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| series | Medicina |
| spelling | doaj.art-5b0249f60dc444f0ae165abc33d4fda72023-09-02T01:53:53ZengMDPI AGMedicina1010-660X2019-02-015525410.3390/medicina55020054medicina55020054Activation of TF-Dependent Blood Coagulation Pathway and VEGF-A in Patients with Essential ThrombocythemiaGrażyna Gadomska0Katarzyna Ziołkowska1Joanna Boinska2Jan Filipiak3Danuta Rość4Department of Hematology and Malignant Diseases of Hematopoietic System, Faculty of Medicine, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, 85-168 Bydgoszcz, PolandDepartment of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, 85-094 Bydgoszcz, PolandDepartment of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, 85-094 Bydgoszcz, PolandDepartment of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, 85-094 Bydgoszcz, PolandDepartment of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, 85-094 Bydgoszcz, Poland<i>Background and objectives:</i> Recent studies suggest that a vascular endothelial growth factor (VEGF-A) may be involved in the thrombotic process by stimulating the expression of tissue factor in vascular endothelial cells. Tissue factor (TF) can also stimulate the transcription of the gene encoding VEGF-A. The relationship between coagulation and angiogenesis in myeloproliferative neoplasms is not fully understood. The aim of this study was to evaluate the concentration of TF in relation to VEGF-A in the blood of patients with essential thrombocythemia (ET). <i>Patients and methods:</i> The study group consisted of 130, newly diagnosed patients with ET (mean age 61 years). The control group consisted of 35 healthy volunteers (mean age 51 years). Concentrations of VEGF-A, TF, and tissue factor pathway inhibitor (TFPI) were analysed using immunoenzymatic methods. TF and TFPI activities were performed using chromogenic assays. <i>Results:</i> The median concentration of TF Ag was 3-fold higher and the TF activity was more than 15-fold higher in ET patients than in normal individuals. There were no statistically significant differences in the TFPI concentration and activity between groups. VEGF-A was significantly increased in patients with ET (<i>p</i> < 0.000001). Analysis of correlations revealed a positive correlation between VEGF-A and TF Ag as well as a positive correlation between VEGF-A and TFPI activity. <i>Conclusions:</i> The simultaneous increase of TF concentration and activity, VEGF-A in the blood of patients with ET, as well as a positive correlation between the concentration of TF and VEGF-A demonstrates the coexistence of TF-dependent coagulation and activation of angiogenesis.https://www.mdpi.com/1010-660X/55/2/54blood coagulationangiogenesistissue factorvascular endothelial growth factor |
| spellingShingle | Grażyna Gadomska Katarzyna Ziołkowska Joanna Boinska Jan Filipiak Danuta Rość Activation of TF-Dependent Blood Coagulation Pathway and VEGF-A in Patients with Essential Thrombocythemia Medicina blood coagulation angiogenesis tissue factor vascular endothelial growth factor |
| title | Activation of TF-Dependent Blood Coagulation Pathway and VEGF-A in Patients with Essential Thrombocythemia |
| title_full | Activation of TF-Dependent Blood Coagulation Pathway and VEGF-A in Patients with Essential Thrombocythemia |
| title_fullStr | Activation of TF-Dependent Blood Coagulation Pathway and VEGF-A in Patients with Essential Thrombocythemia |
| title_full_unstemmed | Activation of TF-Dependent Blood Coagulation Pathway and VEGF-A in Patients with Essential Thrombocythemia |
| title_short | Activation of TF-Dependent Blood Coagulation Pathway and VEGF-A in Patients with Essential Thrombocythemia |
| title_sort | activation of tf dependent blood coagulation pathway and vegf a in patients with essential thrombocythemia |
| topic | blood coagulation angiogenesis tissue factor vascular endothelial growth factor |
| url | https://www.mdpi.com/1010-660X/55/2/54 |
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