Superoxide dismutase, glutathione peroxidase, and catalase antioxidant enzymes in chronic tobacco smokers and chewers: A case–control study

Objective: Tobacco has a time dependent effect on the antioxidant system of the body. This study was designed to determine and compare alteration in levels of erythrocyte superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) in blood subgroups of tobacco smokers and chewers wit...

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Main Authors: Poonam Agarwal, Anjana Bagewadi, Vaishali Keluskar, D P Vinuth
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2019-01-01
Series:Indian Journal of Dental Research
Subjects:
Online Access:http://www.ijdr.in/article.asp?issn=0970-9290;year=2019;volume=30;issue=2;spage=219;epage=225;aulast=Agarwal
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author Poonam Agarwal
Anjana Bagewadi
Vaishali Keluskar
D P Vinuth
author_facet Poonam Agarwal
Anjana Bagewadi
Vaishali Keluskar
D P Vinuth
author_sort Poonam Agarwal
collection DOAJ
description Objective: Tobacco has a time dependent effect on the antioxidant system of the body. This study was designed to determine and compare alteration in levels of erythrocyte superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) in blood subgroups of tobacco smokers and chewers with controls. Materials and Methods: Blood samples were collected from 30 tobacco smokers (> 20 cigarettes daily), 30 tobacco chewers (> 10 packets gutka daily) and 30 controls. These groups were further divided into three subgroups (n=10) based on duration of habit (<5 yrs, 5-10 yrs, >10 yrs). The level of erythrocyte SOD, GPx and CAT were measured using standard procedures. Results: The SOD and CAT levels were significantly decreased in all subgroups of smokers and chewers whereas GPx level was significantly increased. Positive correlation was observed between SOD, GPx and CAT levels with change in duration of habit in all subgroups. No significant difference observed in SOD and CAT activity between tobacco smokers and chewers. Conclusions: The findings suggested that antioxidative enzyme activities have significant correlation with change in the duration of tobacco use. Measurement of markers of free radical activity might be useful for estimating the level of oxidative stress caused by tobacco use.
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spelling doaj.art-5b0253c1def84ff986067741a2ff70a82022-12-21T19:02:21ZengWolters Kluwer Medknow PublicationsIndian Journal of Dental Research0970-92901998-36032019-01-0130221922510.4103/ijdr.IJDR_268_12Superoxide dismutase, glutathione peroxidase, and catalase antioxidant enzymes in chronic tobacco smokers and chewers: A case–control studyPoonam AgarwalAnjana BagewadiVaishali KeluskarD P VinuthObjective: Tobacco has a time dependent effect on the antioxidant system of the body. This study was designed to determine and compare alteration in levels of erythrocyte superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) in blood subgroups of tobacco smokers and chewers with controls. Materials and Methods: Blood samples were collected from 30 tobacco smokers (> 20 cigarettes daily), 30 tobacco chewers (> 10 packets gutka daily) and 30 controls. These groups were further divided into three subgroups (n=10) based on duration of habit (<5 yrs, 5-10 yrs, >10 yrs). The level of erythrocyte SOD, GPx and CAT were measured using standard procedures. Results: The SOD and CAT levels were significantly decreased in all subgroups of smokers and chewers whereas GPx level was significantly increased. Positive correlation was observed between SOD, GPx and CAT levels with change in duration of habit in all subgroups. No significant difference observed in SOD and CAT activity between tobacco smokers and chewers. Conclusions: The findings suggested that antioxidative enzyme activities have significant correlation with change in the duration of tobacco use. Measurement of markers of free radical activity might be useful for estimating the level of oxidative stress caused by tobacco use.http://www.ijdr.in/article.asp?issn=0970-9290;year=2019;volume=30;issue=2;spage=219;epage=225;aulast=AgarwalEnzymatic antioxidantsfree radicalsoxidative stresstobacco
spellingShingle Poonam Agarwal
Anjana Bagewadi
Vaishali Keluskar
D P Vinuth
Superoxide dismutase, glutathione peroxidase, and catalase antioxidant enzymes in chronic tobacco smokers and chewers: A case–control study
Indian Journal of Dental Research
Enzymatic antioxidants
free radicals
oxidative stress
tobacco
title Superoxide dismutase, glutathione peroxidase, and catalase antioxidant enzymes in chronic tobacco smokers and chewers: A case–control study
title_full Superoxide dismutase, glutathione peroxidase, and catalase antioxidant enzymes in chronic tobacco smokers and chewers: A case–control study
title_fullStr Superoxide dismutase, glutathione peroxidase, and catalase antioxidant enzymes in chronic tobacco smokers and chewers: A case–control study
title_full_unstemmed Superoxide dismutase, glutathione peroxidase, and catalase antioxidant enzymes in chronic tobacco smokers and chewers: A case–control study
title_short Superoxide dismutase, glutathione peroxidase, and catalase antioxidant enzymes in chronic tobacco smokers and chewers: A case–control study
title_sort superoxide dismutase glutathione peroxidase and catalase antioxidant enzymes in chronic tobacco smokers and chewers a case control study
topic Enzymatic antioxidants
free radicals
oxidative stress
tobacco
url http://www.ijdr.in/article.asp?issn=0970-9290;year=2019;volume=30;issue=2;spage=219;epage=225;aulast=Agarwal
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AT vaishalikeluskar superoxidedismutaseglutathioneperoxidaseandcatalaseantioxidantenzymesinchronictobaccosmokersandchewersacasecontrolstudy
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