Quantification of Methylisothiazolinone and Methylchloroisothiazolinone Preservatives by High-Performance Liquid Chromatography
Isothiazolinone preservatives (methylisothiazolinone (MIT) and methylchloroisothiazolinone (CMIT) are commonly used in cosmetics, industrial and household products. However, these isothiazolinone derivatives are known to cause allergic contact dermatitis. Hence, a sensitive, accurate, and reliable m...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/4/1760 |
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| author | Samyah Alanazi Hajera Tabassum Manal Abudawood Reem Alrashoudi May Alrashed Yazeed A. Alsheikh Salma Alkaff Manal Alghamdi Naif Alenzi |
| author_facet | Samyah Alanazi Hajera Tabassum Manal Abudawood Reem Alrashoudi May Alrashed Yazeed A. Alsheikh Salma Alkaff Manal Alghamdi Naif Alenzi |
| author_sort | Samyah Alanazi |
| collection | DOAJ |
| description | Isothiazolinone preservatives (methylisothiazolinone (MIT) and methylchloroisothiazolinone (CMIT) are commonly used in cosmetics, industrial and household products. However, these isothiazolinone derivatives are known to cause allergic contact dermatitis. Hence, a sensitive, accurate, and reliable method for the detection of these compounds is thus warranted. The study aims to analyze concentrations of MIT and CMIT by high performance liquid chromatography. The analytical method used for quantification of MIT and CMIT in cosmetic products (leave-on-baby wet wipes) complies with the validation acceptance criteria (international standards ISO 5725, EU25 European Union for cosmetic regulations). MIT and CMIT were extracted and analyzed in leave-on baby wet-wipes collected from different stores in Riyadh city. Extraction was performed by ultrasonication of the samples, solid-phase extraction, and liquid-liquid extraction. Ten (10) µL of the sample was injected into the HPLC system and samples were analyzed with a mixture of acetic acid and methanol (80:20 <i>v</i>/<i>v</i>) in an isocratic mode. The flow rate was maintained at 1 mL/min. UV detection was performed at 274 nm. The results demonstrated recoveries between 90 and 106%, measurement uncertainty of C +/− 0.4% for methylisothiazolinone and C +/− 0.03% for methylchloroisothiazolinone, repeatability limit (r = 0.2%) and intermediate precision limit; R = 2% and R<sup>2</sup> of 0.9996. |
| first_indexed | 2024-03-11T08:20:59Z |
| format | Article |
| id | doaj.art-5b0e1650167649fca88ac5a8b05b77b2 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T08:20:59Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-5b0e1650167649fca88ac5a8b05b77b22023-11-16T22:22:51ZengMDPI AGMolecules1420-30492023-02-01284176010.3390/molecules28041760Quantification of Methylisothiazolinone and Methylchloroisothiazolinone Preservatives by High-Performance Liquid ChromatographySamyah Alanazi0Hajera Tabassum1Manal Abudawood2Reem Alrashoudi3May Alrashed4Yazeed A. Alsheikh5Salma Alkaff6Manal Alghamdi7Naif Alenzi8Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11362, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11362, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11362, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11362, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11362, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11362, Saudi ArabiaDepartment of Chemistry, College of Science and General Studies, Alfaisal University, Riyadh 11553, Saudi ArabiaResearch and Laboratories Sector, National Drug and Cosmetic Control Laboratories (NDCCL), Saudi Food and Drug Authority, Riyadh 13513, Saudi ArabiaResearch and Laboratories Sector, National Drug and Cosmetic Control Laboratories (NDCCL), Saudi Food and Drug Authority, Riyadh 13513, Saudi ArabiaIsothiazolinone preservatives (methylisothiazolinone (MIT) and methylchloroisothiazolinone (CMIT) are commonly used in cosmetics, industrial and household products. However, these isothiazolinone derivatives are known to cause allergic contact dermatitis. Hence, a sensitive, accurate, and reliable method for the detection of these compounds is thus warranted. The study aims to analyze concentrations of MIT and CMIT by high performance liquid chromatography. The analytical method used for quantification of MIT and CMIT in cosmetic products (leave-on-baby wet wipes) complies with the validation acceptance criteria (international standards ISO 5725, EU25 European Union for cosmetic regulations). MIT and CMIT were extracted and analyzed in leave-on baby wet-wipes collected from different stores in Riyadh city. Extraction was performed by ultrasonication of the samples, solid-phase extraction, and liquid-liquid extraction. Ten (10) µL of the sample was injected into the HPLC system and samples were analyzed with a mixture of acetic acid and methanol (80:20 <i>v</i>/<i>v</i>) in an isocratic mode. The flow rate was maintained at 1 mL/min. UV detection was performed at 274 nm. The results demonstrated recoveries between 90 and 106%, measurement uncertainty of C +/− 0.4% for methylisothiazolinone and C +/− 0.03% for methylchloroisothiazolinone, repeatability limit (r = 0.2%) and intermediate precision limit; R = 2% and R<sup>2</sup> of 0.9996.https://www.mdpi.com/1420-3049/28/4/1760preservativesmethylisothiazolinonemethylchloroisothiazolinoneHPLCbaby wipes |
| spellingShingle | Samyah Alanazi Hajera Tabassum Manal Abudawood Reem Alrashoudi May Alrashed Yazeed A. Alsheikh Salma Alkaff Manal Alghamdi Naif Alenzi Quantification of Methylisothiazolinone and Methylchloroisothiazolinone Preservatives by High-Performance Liquid Chromatography Molecules preservatives methylisothiazolinone methylchloroisothiazolinone HPLC baby wipes |
| title | Quantification of Methylisothiazolinone and Methylchloroisothiazolinone Preservatives by High-Performance Liquid Chromatography |
| title_full | Quantification of Methylisothiazolinone and Methylchloroisothiazolinone Preservatives by High-Performance Liquid Chromatography |
| title_fullStr | Quantification of Methylisothiazolinone and Methylchloroisothiazolinone Preservatives by High-Performance Liquid Chromatography |
| title_full_unstemmed | Quantification of Methylisothiazolinone and Methylchloroisothiazolinone Preservatives by High-Performance Liquid Chromatography |
| title_short | Quantification of Methylisothiazolinone and Methylchloroisothiazolinone Preservatives by High-Performance Liquid Chromatography |
| title_sort | quantification of methylisothiazolinone and methylchloroisothiazolinone preservatives by high performance liquid chromatography |
| topic | preservatives methylisothiazolinone methylchloroisothiazolinone HPLC baby wipes |
| url | https://www.mdpi.com/1420-3049/28/4/1760 |
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