Insulin modulates cytokine release and selectin expression in the early phase of allergic airway inflammation in diabetic rats
<p>Abstract</p> <p>Background</p> <p>Clinical and experimental data suggest that the inflammatory response is impaired in diabetics and can be modulated by insulin. The present study was undertaken to investigate the role of insulin on the early phase of allergic airway...
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| Format: | Article |
| Language: | English |
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BMC
2010-07-01
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| Series: | BMC Pulmonary Medicine |
| Online Access: | http://www.biomedcentral.com/1471-2466/10/39 |
| _version_ | 1811284779098701824 |
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| author | Vianna Elcio O Alves Venâncio AF Manzolli Simone Cruz José WMC Campos Carlos AL Martins Joilson O Jancar Sonia Sannomiya Paulina |
| author_facet | Vianna Elcio O Alves Venâncio AF Manzolli Simone Cruz José WMC Campos Carlos AL Martins Joilson O Jancar Sonia Sannomiya Paulina |
| author_sort | Vianna Elcio O |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Clinical and experimental data suggest that the inflammatory response is impaired in diabetics and can be modulated by insulin. The present study was undertaken to investigate the role of insulin on the early phase of allergic airway inflammation.</p> <p>Methods</p> <p>Diabetic male Wistar rats (alloxan, 42 mg/Kg, i.v., 10 days) and controls were sensitized by s.c. injection of ovalbumin (OA) in aluminium hydroxide 14 days before OA (1 mg/0.4 mL) or saline intratracheal challenge. The following analyses were performed 6 hours thereafter: a) quantification of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and cytokine-induced neutrophil chemoattractant (CINC)-1 in the bronchoalveolar lavage fluid (BALF) by Enzyme-Linked Immunosorbent Assay, b) expression of E- and P- selectins on lung vessels by immunohistochemistry, and c) inflammatory cell infiltration into the airways and lung parenchyma. NPH insulin (4 IU, s.c.) was given i.v. 2 hours before antigen challenge.</p> <p>Results</p> <p>Diabetic rats exhibited significant reduction in the BALF concentrations of IL-1β (30%) and TNF-α (45%), and in the lung expression of P-selectin (30%) compared to non-diabetic animals. This was accompanied by reduced number of neutrophils into the airways and around bronchi and blood vessels. There were no differences in the CINC-1 levels in BALF, and E-selectin expression. Treatment of diabetic rats with NPH insulin, 2 hours before antigen challenge, restored the reduced levels of IL-1β, TNF-α and P-selectin, and neutrophil migration.</p> <p>Conclusion</p> <p>Data presented suggest that insulin modulates the production/release of TNF-α and IL-1β, the expression of P- and E-selectin, and the associated neutrophil migration into the lungs during the early phase of the allergic inflammatory reaction.</p> |
| first_indexed | 2024-04-13T02:34:48Z |
| format | Article |
| id | doaj.art-5b10d3fa63f2483583e2f510ea152f88 |
| institution | Directory Open Access Journal |
| issn | 1471-2466 |
| language | English |
| last_indexed | 2024-04-13T02:34:48Z |
| publishDate | 2010-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pulmonary Medicine |
| spelling | doaj.art-5b10d3fa63f2483583e2f510ea152f882022-12-22T03:06:26ZengBMCBMC Pulmonary Medicine1471-24662010-07-011013910.1186/1471-2466-10-39Insulin modulates cytokine release and selectin expression in the early phase of allergic airway inflammation in diabetic ratsVianna Elcio OAlves Venâncio AFManzolli SimoneCruz José WMCCampos Carlos ALMartins Joilson OJancar SoniaSannomiya Paulina<p>Abstract</p> <p>Background</p> <p>Clinical and experimental data suggest that the inflammatory response is impaired in diabetics and can be modulated by insulin. The present study was undertaken to investigate the role of insulin on the early phase of allergic airway inflammation.</p> <p>Methods</p> <p>Diabetic male Wistar rats (alloxan, 42 mg/Kg, i.v., 10 days) and controls were sensitized by s.c. injection of ovalbumin (OA) in aluminium hydroxide 14 days before OA (1 mg/0.4 mL) or saline intratracheal challenge. The following analyses were performed 6 hours thereafter: a) quantification of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and cytokine-induced neutrophil chemoattractant (CINC)-1 in the bronchoalveolar lavage fluid (BALF) by Enzyme-Linked Immunosorbent Assay, b) expression of E- and P- selectins on lung vessels by immunohistochemistry, and c) inflammatory cell infiltration into the airways and lung parenchyma. NPH insulin (4 IU, s.c.) was given i.v. 2 hours before antigen challenge.</p> <p>Results</p> <p>Diabetic rats exhibited significant reduction in the BALF concentrations of IL-1β (30%) and TNF-α (45%), and in the lung expression of P-selectin (30%) compared to non-diabetic animals. This was accompanied by reduced number of neutrophils into the airways and around bronchi and blood vessels. There were no differences in the CINC-1 levels in BALF, and E-selectin expression. Treatment of diabetic rats with NPH insulin, 2 hours before antigen challenge, restored the reduced levels of IL-1β, TNF-α and P-selectin, and neutrophil migration.</p> <p>Conclusion</p> <p>Data presented suggest that insulin modulates the production/release of TNF-α and IL-1β, the expression of P- and E-selectin, and the associated neutrophil migration into the lungs during the early phase of the allergic inflammatory reaction.</p>http://www.biomedcentral.com/1471-2466/10/39 |
| spellingShingle | Vianna Elcio O Alves Venâncio AF Manzolli Simone Cruz José WMC Campos Carlos AL Martins Joilson O Jancar Sonia Sannomiya Paulina Insulin modulates cytokine release and selectin expression in the early phase of allergic airway inflammation in diabetic rats BMC Pulmonary Medicine |
| title | Insulin modulates cytokine release and selectin expression in the early phase of allergic airway inflammation in diabetic rats |
| title_full | Insulin modulates cytokine release and selectin expression in the early phase of allergic airway inflammation in diabetic rats |
| title_fullStr | Insulin modulates cytokine release and selectin expression in the early phase of allergic airway inflammation in diabetic rats |
| title_full_unstemmed | Insulin modulates cytokine release and selectin expression in the early phase of allergic airway inflammation in diabetic rats |
| title_short | Insulin modulates cytokine release and selectin expression in the early phase of allergic airway inflammation in diabetic rats |
| title_sort | insulin modulates cytokine release and selectin expression in the early phase of allergic airway inflammation in diabetic rats |
| url | http://www.biomedcentral.com/1471-2466/10/39 |
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