Antibiotic resistance and mecA gene characterization of Staphylococcus epidermidis isolated from some hospitals in Gaza strip
Antibiotic resistance of S. epidermidis isolated from biological specimens is a global problem to public health. In this study a total of 256 S. epidermidis isolates (128 clinical isolates and 128 nasal isolates) from Gaza strip, Palestine were investigated. All isolates were tested for its antimicr...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Ain Shams University, Faculty of Women for Arts, Science & Education
2020-12-01
|
| Series: | Journal of Scientific Research in Science |
| Subjects: | |
| Online Access: | https://jsrs.journals.ekb.eg/article_129931.html |
| _version_ | 1797736681902702592 |
|---|---|
| author | Mohanad A. Alnajar Shimaa M. Abdelsalam Nabil A. El Aila Basim M. Ayesh Mona I. Fahd |
| author_facet | Mohanad A. Alnajar Shimaa M. Abdelsalam Nabil A. El Aila Basim M. Ayesh Mona I. Fahd |
| author_sort | Mohanad A. Alnajar |
| collection | DOAJ |
| description | Antibiotic resistance of S. epidermidis isolated from biological specimens is a global problem to public health. In this study a total of 256 S. epidermidis isolates (128 clinical isolates and 128 nasal isolates) from Gaza strip, Palestine were investigated. All isolates were tested for its antimicrobial susceptibilities and carriage of the mecA gene. Out of the 256 isolates, 184 (71.9%) were resistant to multiple antibiotics with all displaying increased susceptibility toward rifampicin (100%), doxycycline (98.4%) and vancomycin (98%). Ninety-six isolates (37.5%) were multidrug resistant (MDR) while, 99 isolates (38.7%) were mecA positive. A significant difference was demonstrated between clinical and nasal isolates. Clinical isolates were significantly more resistant for 8/12 tested antibiotics including resistance to cefoxitin (30μg) (p=0.000) and significantly (p=0.000) represents the MDR isolates while nasal isolates were significantly (p=0.000) sensitive for all tested antibiotics. No significant difference between the two groups in carrying mecA. We find that clinical isolates gain an extra-feature that qualify it to cause a disease and methicillin resistance (MR) was not mecA dependent in all MR isolates. |
| first_indexed | 2024-03-12T13:17:24Z |
| format | Article |
| id | doaj.art-5b136dab8ee04cbabecde95841d19eea |
| institution | Directory Open Access Journal |
| issn | 2356-8364 2356-8372 |
| language | English |
| last_indexed | 2024-03-12T13:17:24Z |
| publishDate | 2020-12-01 |
| publisher | Ain Shams University, Faculty of Women for Arts, Science & Education |
| record_format | Article |
| series | Journal of Scientific Research in Science |
| spelling | doaj.art-5b136dab8ee04cbabecde95841d19eea2023-08-26T14:31:27ZengAin Shams University, Faculty of Women for Arts, Science & EducationJournal of Scientific Research in Science2356-83642356-83722020-12-013723046doi:10.21608/JSRS.2020.129931Antibiotic resistance and mecA gene characterization of Staphylococcus epidermidis isolated from some hospitals in Gaza stripMohanad A. Alnajar0Shimaa M. Abdelsalam1Nabil A. El Aila2Basim M. Ayesh3Mona I. Fahd4Medical Sciences department, University Collage of Science and Technology, Gaza Strip, Palestine.Botany department, Faculty of Women for Arts, Sciences and Education, Ain Shams University, Cairo, Egypt.Laboratory Medical Sciences department, Faculty of Medical Sciences, Al-Aqsa University, Gaza Strip, Palestine.Laboratory Medical Sciences department, Faculty of Medical Sciences, Al-Aqsa University, Gaza Strip, Palestine.Botany department, Faculty of Women for Arts, Sciences and Education, Ain Shams University, Cairo, Egypt.Antibiotic resistance of S. epidermidis isolated from biological specimens is a global problem to public health. In this study a total of 256 S. epidermidis isolates (128 clinical isolates and 128 nasal isolates) from Gaza strip, Palestine were investigated. All isolates were tested for its antimicrobial susceptibilities and carriage of the mecA gene. Out of the 256 isolates, 184 (71.9%) were resistant to multiple antibiotics with all displaying increased susceptibility toward rifampicin (100%), doxycycline (98.4%) and vancomycin (98%). Ninety-six isolates (37.5%) were multidrug resistant (MDR) while, 99 isolates (38.7%) were mecA positive. A significant difference was demonstrated between clinical and nasal isolates. Clinical isolates were significantly more resistant for 8/12 tested antibiotics including resistance to cefoxitin (30μg) (p=0.000) and significantly (p=0.000) represents the MDR isolates while nasal isolates were significantly (p=0.000) sensitive for all tested antibiotics. No significant difference between the two groups in carrying mecA. We find that clinical isolates gain an extra-feature that qualify it to cause a disease and methicillin resistance (MR) was not mecA dependent in all MR isolates.https://jsrs.journals.ekb.eg/article_129931.htmlstaphylococcus epidermidisantibiotic resistancemultidrug resistancemethicillin resistancemeca gene. |
| spellingShingle | Mohanad A. Alnajar Shimaa M. Abdelsalam Nabil A. El Aila Basim M. Ayesh Mona I. Fahd Antibiotic resistance and mecA gene characterization of Staphylococcus epidermidis isolated from some hospitals in Gaza strip Journal of Scientific Research in Science staphylococcus epidermidis antibiotic resistance multidrug resistance methicillin resistance meca gene. |
| title | Antibiotic resistance and mecA gene characterization of Staphylococcus epidermidis isolated from some hospitals in Gaza strip |
| title_full | Antibiotic resistance and mecA gene characterization of Staphylococcus epidermidis isolated from some hospitals in Gaza strip |
| title_fullStr | Antibiotic resistance and mecA gene characterization of Staphylococcus epidermidis isolated from some hospitals in Gaza strip |
| title_full_unstemmed | Antibiotic resistance and mecA gene characterization of Staphylococcus epidermidis isolated from some hospitals in Gaza strip |
| title_short | Antibiotic resistance and mecA gene characterization of Staphylococcus epidermidis isolated from some hospitals in Gaza strip |
| title_sort | antibiotic resistance and meca gene characterization of staphylococcus epidermidis isolated from some hospitals in gaza strip |
| topic | staphylococcus epidermidis antibiotic resistance multidrug resistance methicillin resistance meca gene. |
| url | https://jsrs.journals.ekb.eg/article_129931.html |
| work_keys_str_mv | AT mohanadaalnajar antibioticresistanceandmecagenecharacterizationofstaphylococcusepidermidisisolatedfromsomehospitalsingazastrip AT shimaamabdelsalam antibioticresistanceandmecagenecharacterizationofstaphylococcusepidermidisisolatedfromsomehospitalsingazastrip AT nabilaelaila antibioticresistanceandmecagenecharacterizationofstaphylococcusepidermidisisolatedfromsomehospitalsingazastrip AT basimmayesh antibioticresistanceandmecagenecharacterizationofstaphylococcusepidermidisisolatedfromsomehospitalsingazastrip AT monaifahd antibioticresistanceandmecagenecharacterizationofstaphylococcusepidermidisisolatedfromsomehospitalsingazastrip |