Chitosan and Sodium Hyaluronate Hydrogels Supplemented with Bioglass for Bone Tissue Engineering
The aim of the study was to produce biocomposites based on chitosan and sodium hyaluronate hydrogels supplemented with bioglasses obtained under different conditions (temperature, time) and to perform an in vitro evaluation of their cytocompatibility using both indirect and direct methods. Furthermo...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
|
| Series: | Gels |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2310-2861/10/2/128 |
| _version_ | 1797298140119826432 |
|---|---|
| author | Lidia Ciołek Ewa Zaczyńska Małgorzata Krok-Borkowicz Monika Biernat Elżbieta Pamuła |
| author_facet | Lidia Ciołek Ewa Zaczyńska Małgorzata Krok-Borkowicz Monika Biernat Elżbieta Pamuła |
| author_sort | Lidia Ciołek |
| collection | DOAJ |
| description | The aim of the study was to produce biocomposites based on chitosan and sodium hyaluronate hydrogels supplemented with bioglasses obtained under different conditions (temperature, time) and to perform an in vitro evaluation of their cytocompatibility using both indirect and direct methods. Furthermore, the release of ions from the composites and the microstructure of the biocomposites before and after incubation in simulated body fluid were assessed. Tests on extracts from bioglasses and hydrogel biocomposites were performed on A549 epithelial cells, while MG63 osteoblast-like cells were tested in direct contact with the developed biomaterials. The immune response induced by the biomaterials was also evaluated. The experiments were carried out on both unstimulated and lipopolysaccharide (LPS) endotoxin-stimulated human peripheral blood cells in the presence of extracts of the biocomposites and their components. Extracts of the materials produced do not exhibit toxic effects on A549 cells, and do not increase the production of proinflammatory cytokines tumour necrosis factor alpha (TNF-α) and interleukin (IL-6) by blood cells in vitro. In direct contact with MG63 osteoblast-like cells, biocomposites containing the reference bioglass and those containing SrO are more cytocompatible than biocomposites with ZnO-doped bioglass. Using two testing approaches, the effects both of the potentially toxic agents released and of the surface of the tested materials on the cell condition were assessed. The results pave the way for the development of highly porous hydrogel–bioglass composite scaffolds for bone tissue engineering. |
| first_indexed | 2024-03-07T22:31:37Z |
| format | Article |
| id | doaj.art-5b150d128a14434b901e70bcc26b8a29 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-07T22:31:37Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-5b150d128a14434b901e70bcc26b8a292024-02-23T15:17:32ZengMDPI AGGels2310-28612024-02-0110212810.3390/gels10020128Chitosan and Sodium Hyaluronate Hydrogels Supplemented with Bioglass for Bone Tissue EngineeringLidia Ciołek0Ewa Zaczyńska1Małgorzata Krok-Borkowicz2Monika Biernat3Elżbieta Pamuła4Biomaterials Research Group, Łukasiewicz Research Network—Institute of Ceramic and Building Materials, 31-983 Krakow, PolandHirszfeld Institute of Immunology and Experimental Therapy, Laboratory of Immunobiology, Polish Academy of Sciences, R. Weigla Str. 12, 53-114 Wroclaw, PolandDepartment of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Krakow, Al. Mickiewicza 30, 30-059 Krakow, PolandBiomaterials Research Group, Łukasiewicz Research Network—Institute of Ceramic and Building Materials, 31-983 Krakow, PolandDepartment of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Krakow, Al. Mickiewicza 30, 30-059 Krakow, PolandThe aim of the study was to produce biocomposites based on chitosan and sodium hyaluronate hydrogels supplemented with bioglasses obtained under different conditions (temperature, time) and to perform an in vitro evaluation of their cytocompatibility using both indirect and direct methods. Furthermore, the release of ions from the composites and the microstructure of the biocomposites before and after incubation in simulated body fluid were assessed. Tests on extracts from bioglasses and hydrogel biocomposites were performed on A549 epithelial cells, while MG63 osteoblast-like cells were tested in direct contact with the developed biomaterials. The immune response induced by the biomaterials was also evaluated. The experiments were carried out on both unstimulated and lipopolysaccharide (LPS) endotoxin-stimulated human peripheral blood cells in the presence of extracts of the biocomposites and their components. Extracts of the materials produced do not exhibit toxic effects on A549 cells, and do not increase the production of proinflammatory cytokines tumour necrosis factor alpha (TNF-α) and interleukin (IL-6) by blood cells in vitro. In direct contact with MG63 osteoblast-like cells, biocomposites containing the reference bioglass and those containing SrO are more cytocompatible than biocomposites with ZnO-doped bioglass. Using two testing approaches, the effects both of the potentially toxic agents released and of the surface of the tested materials on the cell condition were assessed. The results pave the way for the development of highly porous hydrogel–bioglass composite scaffolds for bone tissue engineering.https://www.mdpi.com/2310-2861/10/2/128chitosanhyaluronic acidbioglassbiocompositescytocompatibilitypro-inflammatory cytokines |
| spellingShingle | Lidia Ciołek Ewa Zaczyńska Małgorzata Krok-Borkowicz Monika Biernat Elżbieta Pamuła Chitosan and Sodium Hyaluronate Hydrogels Supplemented with Bioglass for Bone Tissue Engineering Gels chitosan hyaluronic acid bioglass biocomposites cytocompatibility pro-inflammatory cytokines |
| title | Chitosan and Sodium Hyaluronate Hydrogels Supplemented with Bioglass for Bone Tissue Engineering |
| title_full | Chitosan and Sodium Hyaluronate Hydrogels Supplemented with Bioglass for Bone Tissue Engineering |
| title_fullStr | Chitosan and Sodium Hyaluronate Hydrogels Supplemented with Bioglass for Bone Tissue Engineering |
| title_full_unstemmed | Chitosan and Sodium Hyaluronate Hydrogels Supplemented with Bioglass for Bone Tissue Engineering |
| title_short | Chitosan and Sodium Hyaluronate Hydrogels Supplemented with Bioglass for Bone Tissue Engineering |
| title_sort | chitosan and sodium hyaluronate hydrogels supplemented with bioglass for bone tissue engineering |
| topic | chitosan hyaluronic acid bioglass biocomposites cytocompatibility pro-inflammatory cytokines |
| url | https://www.mdpi.com/2310-2861/10/2/128 |
| work_keys_str_mv | AT lidiaciołek chitosanandsodiumhyaluronatehydrogelssupplementedwithbioglassforbonetissueengineering AT ewazaczynska chitosanandsodiumhyaluronatehydrogelssupplementedwithbioglassforbonetissueengineering AT małgorzatakrokborkowicz chitosanandsodiumhyaluronatehydrogelssupplementedwithbioglassforbonetissueengineering AT monikabiernat chitosanandsodiumhyaluronatehydrogelssupplementedwithbioglassforbonetissueengineering AT elzbietapamuła chitosanandsodiumhyaluronatehydrogelssupplementedwithbioglassforbonetissueengineering |