Molecular characterization of cell types in the squid Loligo vulgaris
Cephalopods are set apart from other mollusks by their advanced behavioral abilities and the complexity of their nervous systems. Because of the great evolutionary distance that separates vertebrates from cephalopods, it is evident that higher cognitive features have evolved separately in these clad...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2023-01-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/80670 |
| _version_ | 1797953521798086656 |
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| author | Jules Duruz Marta Sprecher Jenifer C Kaldun Al-Sayed Al-Soudy Heidi EL Lischer Geert van Geest Pamela Nicholson Rémy Bruggmann Simon G Sprecher |
| author_facet | Jules Duruz Marta Sprecher Jenifer C Kaldun Al-Sayed Al-Soudy Heidi EL Lischer Geert van Geest Pamela Nicholson Rémy Bruggmann Simon G Sprecher |
| author_sort | Jules Duruz |
| collection | DOAJ |
| description | Cephalopods are set apart from other mollusks by their advanced behavioral abilities and the complexity of their nervous systems. Because of the great evolutionary distance that separates vertebrates from cephalopods, it is evident that higher cognitive features have evolved separately in these clades despite the similarities that they share. Alongside their complex behavioral abilities, cephalopods have evolved specialized cells and tissues, such as the chromatophores for camouflage or suckers to grasp prey. Despite significant progress in genome and transcriptome sequencing, the molecular identities of cell types in cephalopods remain largely unknown. We here combine single-cell transcriptomics with in situ gene expression analysis to uncover cell type diversity in the European squid Loligo vulgaris. We describe cell types that are conserved with other phyla such as neurons, muscles, or connective tissues but also cephalopod-specific cells, such as chromatophores or sucker cells. Moreover, we investigate major components of the squid nervous system including progenitor and developing cells, differentiated cells of the brain and optic lobes, as well as sensory systems of the head. Our study provides a molecular assessment for conserved and novel cell types in cephalopods and a framework for mapping the nervous system of L. vulgaris. |
| first_indexed | 2024-04-10T23:03:21Z |
| format | Article |
| id | doaj.art-5b153e15d9f04edf9dfea1fa3c4bb2ee |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-10T23:03:21Z |
| publishDate | 2023-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-5b153e15d9f04edf9dfea1fa3c4bb2ee2023-01-13T16:29:29ZengeLife Sciences Publications LtdeLife2050-084X2023-01-011210.7554/eLife.80670Molecular characterization of cell types in the squid Loligo vulgarisJules Duruz0https://orcid.org/0000-0003-1860-9546Marta Sprecher1Jenifer C Kaldun2Al-Sayed Al-Soudy3https://orcid.org/0000-0002-7830-9660Heidi EL Lischer4https://orcid.org/0000-0002-9616-2092Geert van Geest5https://orcid.org/0000-0002-1561-078XPamela Nicholson6Rémy Bruggmann7https://orcid.org/0000-0003-4733-7922Simon G Sprecher8https://orcid.org/0000-0001-9060-3750Department of Biology, Institute of Zoology, University of Fribourg, Fribourg, SwitzerlandDepartment of Biology, Institute of Zoology, University of Fribourg, Fribourg, SwitzerlandDepartment of Biology, Institute of Zoology, University of Fribourg, Fribourg, SwitzerlandDepartment of Biology, Institute of Zoology, University of Fribourg, Fribourg, SwitzerlandInterfaculty Bioinformatics Unit and Swiss Institute of Bioinformatics, University of Bern, Bern, SwitzerlandInterfaculty Bioinformatics Unit and Swiss Institute of Bioinformatics, University of Bern, Bern, SwitzerlandInstitute of Genetics, University of Bern, Bern, SwitzerlandInterfaculty Bioinformatics Unit and Swiss Institute of Bioinformatics, University of Bern, Bern, SwitzerlandDepartment of Biology, Institute of Zoology, University of Fribourg, Fribourg, SwitzerlandCephalopods are set apart from other mollusks by their advanced behavioral abilities and the complexity of their nervous systems. Because of the great evolutionary distance that separates vertebrates from cephalopods, it is evident that higher cognitive features have evolved separately in these clades despite the similarities that they share. Alongside their complex behavioral abilities, cephalopods have evolved specialized cells and tissues, such as the chromatophores for camouflage or suckers to grasp prey. Despite significant progress in genome and transcriptome sequencing, the molecular identities of cell types in cephalopods remain largely unknown. We here combine single-cell transcriptomics with in situ gene expression analysis to uncover cell type diversity in the European squid Loligo vulgaris. We describe cell types that are conserved with other phyla such as neurons, muscles, or connective tissues but also cephalopod-specific cells, such as chromatophores or sucker cells. Moreover, we investigate major components of the squid nervous system including progenitor and developing cells, differentiated cells of the brain and optic lobes, as well as sensory systems of the head. Our study provides a molecular assessment for conserved and novel cell types in cephalopods and a framework for mapping the nervous system of L. vulgaris.https://elifesciences.org/articles/80670cephalopodLoligo vulgarissingle-cell RNAseqcell typeevolution |
| spellingShingle | Jules Duruz Marta Sprecher Jenifer C Kaldun Al-Sayed Al-Soudy Heidi EL Lischer Geert van Geest Pamela Nicholson Rémy Bruggmann Simon G Sprecher Molecular characterization of cell types in the squid Loligo vulgaris eLife cephalopod Loligo vulgaris single-cell RNAseq cell type evolution |
| title | Molecular characterization of cell types in the squid Loligo vulgaris |
| title_full | Molecular characterization of cell types in the squid Loligo vulgaris |
| title_fullStr | Molecular characterization of cell types in the squid Loligo vulgaris |
| title_full_unstemmed | Molecular characterization of cell types in the squid Loligo vulgaris |
| title_short | Molecular characterization of cell types in the squid Loligo vulgaris |
| title_sort | molecular characterization of cell types in the squid loligo vulgaris |
| topic | cephalopod Loligo vulgaris single-cell RNAseq cell type evolution |
| url | https://elifesciences.org/articles/80670 |
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