Integrating transcriptomic and metabolomic analysis in roots of wild soybean seedlings in response to low-phosphorus stress
IntroductionPlants undergo divergent adaptations to form different ecotypes when exposed to different habitats. Ecotypes with ecological adaptation advantages are excellent germplasm resources for crop improvementMethodshis study comprehensively compared the differences in morphology and physiologic...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1006806/full |
| _version_ | 1798017368630231040 |
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| author | Mingxia Li Ji Zhou Xianyu Lang Defu Han Yongjun Hu Yinan Ding Guangye Wang Jixun Guo Lianxuan Shi |
| author_facet | Mingxia Li Ji Zhou Xianyu Lang Defu Han Yongjun Hu Yinan Ding Guangye Wang Jixun Guo Lianxuan Shi |
| author_sort | Mingxia Li |
| collection | DOAJ |
| description | IntroductionPlants undergo divergent adaptations to form different ecotypes when exposed to different habitats. Ecotypes with ecological adaptation advantages are excellent germplasm resources for crop improvementMethodshis study comprehensively compared the differences in morphology and physiological mechanisms in the roots of two different ecotypes of wild soybean (Glycine soja) seedlings under artificially simulated low-phosphorus (LP) stress.ResultThe seedlings of barren-tolerant wild soybean (GS2) suffered less damage than common wild soybean (GS1). GS2 absorbed more phosphorus (P) by increasing root length. In-depth integrated analyses of transcriptomics and metabolomics revealed the formation process of the ecological adaptability of the two different ecotypes wild soybean from the perspective of gene expression and metabolic changes. This study revealed the adaptation process of GS2 from the perspective of the adaptation of structural and molecular metabolism, mainly including: (1) Enhancing the metabolism of phenolic compounds, lignin, and organic acid metabolism could activate unavailable soil P; (2) Up-regulating genes encoding pectinesterase and phospholipase C (PLC) specifically could promote the reuse of structural P; (3) Some factors could reduce the oxidative damage to the membranes caused by LP stress, such as accumulating the metabolites putrescine and ascorbate significantly, up-regulating the genes encoding SQD2 (the key enzyme of sulfolipid substitution of phospholipids) substantially and enhancing the synthesis of secondary antioxidant metabolite anthocyanins and the AsA-GSH cycle; (4) enhancing the uptake of soil P by upregulating inorganic phosphate transporter, acid phosphatase ACP1, and purple acid phosphatase genes; (5) HSFA6b and MYB61 are the key TFs to resist LP stress.DiscussionIn general, GS2 could resist LP stress by activating unavailable soil P, reusing plant structural P, rebuilding membrane lipids, and enhancing the antioxidant membrane protection system. Our study provides a new perspective for the study of divergent adaptation of plants. |
| first_indexed | 2024-04-11T16:06:07Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T16:06:07Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-5b1aee2782c64204852eb95631a640da2022-12-22T04:14:49ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-11-011310.3389/fpls.2022.10068061006806Integrating transcriptomic and metabolomic analysis in roots of wild soybean seedlings in response to low-phosphorus stressMingxia Li0Ji Zhou1Xianyu Lang2Defu Han3Yongjun Hu4Yinan Ding5Guangye Wang6Jixun Guo7Lianxuan Shi8School of Life Sciences, Changchun Normal University, Changchun, ChinaLand Consolidation and Rehabilitation Centre, The Ministry of Land and Resources, Beijing, ChinaSchool of Life Sciences, Changchun Normal University, Changchun, ChinaSchool of Life Sciences, Changchun Normal University, Changchun, ChinaSchool of Life Sciences, Changchun Normal University, Changchun, ChinaSchool of Life Sciences, Changchun Normal University, Changchun, ChinaSchool of Life Sciences, Changchun Normal University, Changchun, ChinaInstitute of Grassland Science, Northeast Normal University, Key Laboratory of Vegetation Ecology, Ministry of Education, Changchun, ChinaInstitute of Grassland Science, Northeast Normal University, Key Laboratory of Vegetation Ecology, Ministry of Education, Changchun, ChinaIntroductionPlants undergo divergent adaptations to form different ecotypes when exposed to different habitats. Ecotypes with ecological adaptation advantages are excellent germplasm resources for crop improvementMethodshis study comprehensively compared the differences in morphology and physiological mechanisms in the roots of two different ecotypes of wild soybean (Glycine soja) seedlings under artificially simulated low-phosphorus (LP) stress.ResultThe seedlings of barren-tolerant wild soybean (GS2) suffered less damage than common wild soybean (GS1). GS2 absorbed more phosphorus (P) by increasing root length. In-depth integrated analyses of transcriptomics and metabolomics revealed the formation process of the ecological adaptability of the two different ecotypes wild soybean from the perspective of gene expression and metabolic changes. This study revealed the adaptation process of GS2 from the perspective of the adaptation of structural and molecular metabolism, mainly including: (1) Enhancing the metabolism of phenolic compounds, lignin, and organic acid metabolism could activate unavailable soil P; (2) Up-regulating genes encoding pectinesterase and phospholipase C (PLC) specifically could promote the reuse of structural P; (3) Some factors could reduce the oxidative damage to the membranes caused by LP stress, such as accumulating the metabolites putrescine and ascorbate significantly, up-regulating the genes encoding SQD2 (the key enzyme of sulfolipid substitution of phospholipids) substantially and enhancing the synthesis of secondary antioxidant metabolite anthocyanins and the AsA-GSH cycle; (4) enhancing the uptake of soil P by upregulating inorganic phosphate transporter, acid phosphatase ACP1, and purple acid phosphatase genes; (5) HSFA6b and MYB61 are the key TFs to resist LP stress.DiscussionIn general, GS2 could resist LP stress by activating unavailable soil P, reusing plant structural P, rebuilding membrane lipids, and enhancing the antioxidant membrane protection system. Our study provides a new perspective for the study of divergent adaptation of plants.https://www.frontiersin.org/articles/10.3389/fpls.2022.1006806/fulladaptationsmetabolomicsphosphorus deficiencytranscriptomicswild soybean |
| spellingShingle | Mingxia Li Ji Zhou Xianyu Lang Defu Han Yongjun Hu Yinan Ding Guangye Wang Jixun Guo Lianxuan Shi Integrating transcriptomic and metabolomic analysis in roots of wild soybean seedlings in response to low-phosphorus stress Frontiers in Plant Science adaptations metabolomics phosphorus deficiency transcriptomics wild soybean |
| title | Integrating transcriptomic and metabolomic analysis in roots of wild soybean seedlings in response to low-phosphorus stress |
| title_full | Integrating transcriptomic and metabolomic analysis in roots of wild soybean seedlings in response to low-phosphorus stress |
| title_fullStr | Integrating transcriptomic and metabolomic analysis in roots of wild soybean seedlings in response to low-phosphorus stress |
| title_full_unstemmed | Integrating transcriptomic and metabolomic analysis in roots of wild soybean seedlings in response to low-phosphorus stress |
| title_short | Integrating transcriptomic and metabolomic analysis in roots of wild soybean seedlings in response to low-phosphorus stress |
| title_sort | integrating transcriptomic and metabolomic analysis in roots of wild soybean seedlings in response to low phosphorus stress |
| topic | adaptations metabolomics phosphorus deficiency transcriptomics wild soybean |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1006806/full |
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