Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers
Cellular barriers, such as the skin, the lung epithelium or the intestinal epithelium, constitute one of the first obstacles facing nanomedicines or other nanoparticles entering organisms. It is thus important to assess the capacity of nanoparticles to enter and transport across such barriers. In th...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Beilstein-Institut
2017-07-01
|
| Series: | Beilstein Journal of Nanotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.3762/bjnano.8.141 |
| _version_ | 1818056837958729728 |
|---|---|
| author | Dong Ye Mattia Bramini Delyan R. Hristov Sha Wan Anna Salvati Christoffer Åberg Kenneth A. Dawson |
| author_facet | Dong Ye Mattia Bramini Delyan R. Hristov Sha Wan Anna Salvati Christoffer Åberg Kenneth A. Dawson |
| author_sort | Dong Ye |
| collection | DOAJ |
| description | Cellular barriers, such as the skin, the lung epithelium or the intestinal epithelium, constitute one of the first obstacles facing nanomedicines or other nanoparticles entering organisms. It is thus important to assess the capacity of nanoparticles to enter and transport across such barriers. In this work, Caco-2 intestinal epithelial cells were used as a well-established model for the intestinal barrier, and the uptake, trafficking and translocation of model silica nanoparticles of different sizes were investigated using a combination of imaging, flow cytometry and transport studies. Compared to typical observations in standard cell lines commonly used for in vitro studies, silica nanoparticle uptake into well-developed Caco-2 cellular barriers was found to be very low. Instead, nanoparticle association to the apical outer membrane was substantial and these particles could easily be misinterpreted as internalised in the absence of imaging. Passage of nanoparticles through the barrier was very limited, suggesting that the low amount of internalised nanoparticles was due to reduced uptake into cells, rather than a considerable transport through them. |
| first_indexed | 2024-12-10T12:35:12Z |
| format | Article |
| id | doaj.art-5b1e09a842c346b49b9ba8047592cdb4 |
| institution | Directory Open Access Journal |
| issn | 2190-4286 |
| language | English |
| last_indexed | 2024-12-10T12:35:12Z |
| publishDate | 2017-07-01 |
| publisher | Beilstein-Institut |
| record_format | Article |
| series | Beilstein Journal of Nanotechnology |
| spelling | doaj.art-5b1e09a842c346b49b9ba8047592cdb42022-12-22T01:48:41ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862017-07-01811396140610.3762/bjnano.8.1412190-4286-8-141Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriersDong Ye0Mattia Bramini1Delyan R. Hristov2Sha Wan3Anna Salvati4Christoffer Åberg5Kenneth A. Dawson6Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, IrelandCentre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, IrelandCentre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, IrelandCentre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, IrelandCentre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, IrelandCentre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, IrelandCentre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, IrelandCellular barriers, such as the skin, the lung epithelium or the intestinal epithelium, constitute one of the first obstacles facing nanomedicines or other nanoparticles entering organisms. It is thus important to assess the capacity of nanoparticles to enter and transport across such barriers. In this work, Caco-2 intestinal epithelial cells were used as a well-established model for the intestinal barrier, and the uptake, trafficking and translocation of model silica nanoparticles of different sizes were investigated using a combination of imaging, flow cytometry and transport studies. Compared to typical observations in standard cell lines commonly used for in vitro studies, silica nanoparticle uptake into well-developed Caco-2 cellular barriers was found to be very low. Instead, nanoparticle association to the apical outer membrane was substantial and these particles could easily be misinterpreted as internalised in the absence of imaging. Passage of nanoparticles through the barrier was very limited, suggesting that the low amount of internalised nanoparticles was due to reduced uptake into cells, rather than a considerable transport through them.https://doi.org/10.3762/bjnano.8.141Caco-2differentiation and polarisationepithelial cell barriermicroscopy imagingparticle interactionuptake and localisation |
| spellingShingle | Dong Ye Mattia Bramini Delyan R. Hristov Sha Wan Anna Salvati Christoffer Åberg Kenneth A. Dawson Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers Beilstein Journal of Nanotechnology Caco-2 differentiation and polarisation epithelial cell barrier microscopy imaging particle interaction uptake and localisation |
| title | Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers |
| title_full | Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers |
| title_fullStr | Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers |
| title_full_unstemmed | Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers |
| title_short | Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers |
| title_sort | low uptake of silica nanoparticles in caco 2 intestinal epithelial barriers |
| topic | Caco-2 differentiation and polarisation epithelial cell barrier microscopy imaging particle interaction uptake and localisation |
| url | https://doi.org/10.3762/bjnano.8.141 |
| work_keys_str_mv | AT dongye lowuptakeofsilicananoparticlesincaco2intestinalepithelialbarriers AT mattiabramini lowuptakeofsilicananoparticlesincaco2intestinalepithelialbarriers AT delyanrhristov lowuptakeofsilicananoparticlesincaco2intestinalepithelialbarriers AT shawan lowuptakeofsilicananoparticlesincaco2intestinalepithelialbarriers AT annasalvati lowuptakeofsilicananoparticlesincaco2intestinalepithelialbarriers AT christofferaberg lowuptakeofsilicananoparticlesincaco2intestinalepithelialbarriers AT kennethadawson lowuptakeofsilicananoparticlesincaco2intestinalepithelialbarriers |