Effect of quercetin or butylated hydroxytoluene on cooled or frozen-thawed ram sperm quality
Cooling and freezing processes cause physical and chemical damage to sperm by cold shock and oxidative stress. This study aimed to evaluate the effect of two antioxidants on sperm parameters of cooled and frozen-thawed ram semen diluted in an egg yolk-based extender. Semen was collected from 30 rams...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2022-02-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44068 |
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| author | Lucas Emanuel Ferreira Canuto Lorenzo Garrido Teixeira Martini Segabinazzi Endrigo Adonis Braga de Araújo Luis Fernando Mercês Chaves Silva Sidnei Nunes de Oliveira Felipe Morales Dalanezi José Antonio Dell'Aqua Junior Frederico Ozanam Papa Eunice Oba |
| author_facet | Lucas Emanuel Ferreira Canuto Lorenzo Garrido Teixeira Martini Segabinazzi Endrigo Adonis Braga de Araújo Luis Fernando Mercês Chaves Silva Sidnei Nunes de Oliveira Felipe Morales Dalanezi José Antonio Dell'Aqua Junior Frederico Ozanam Papa Eunice Oba |
| author_sort | Lucas Emanuel Ferreira Canuto |
| collection | DOAJ |
| description | Cooling and freezing processes cause physical and chemical damage to sperm by cold shock and oxidative stress. This study aimed to evaluate the effect of two antioxidants on sperm parameters of cooled and frozen-thawed ram semen diluted in an egg yolk-based extender. Semen was collected from 30 rams and processed in two consecutive experiments to test the inclusion of different concentrations of quercetin and butylated hydroxytoluene (BHT) in an egg yolk-based semen extender. Dimethyl sulfoxide (DMSO) was added as a solvent to the semen extender in a ratio of 1 mL DMSO for 90 mg of quercetin and 1 mL DMSO for 880 mg of BHT. After collection, semen was diluted at 200 × 106 motile sperm/mL (control) and split into different groups in each experiment. In experiment 1, semen was diluted with the extender containing quercetin (Q5, 5 µg/mL; Q10, 10 ug/mL; Q15, 15 ug/mL) or DMSO alone (DMSO1, 0.055 uL DMSO per mL; DMSO2, 0.165 uL DMSO per mL). In experiment 2, semen was diluted with the extender with BHT (BHT1, 0.5 ug/mL; BHT2, 1 ug/mL; BHT3, 1.5 ug/mL) or DMSO alone (DMSO3, 0.375 uL DMSO per mL; DMSO4, 1.125 uL DMSO per mL). After dilution, the semen was divided into two aliquots. Treated ram sperm samples were also subjected to different storage methods. The first set of samples was cooled at 5 °C for 24 h, whereas the second set of samples was frozen-thawed. Sperm motility parameters and plasma membrane integrity (PMI) were evaluated immediately after dilution (0h) and 24 h after cooling and in the frozen-thawed samples via computer-assisted sperm analysis and epifluorescence microscopy, respectively. The inclusion of quercetin or BHT did not affect sperm motility parameters or PMI of fresh, cooled, or frozen-thawed sperm in this study (P < 0.05). However, further studies are needed to test the effects of these antioxidants on the fertility of cryopreserved ram semen. |
| first_indexed | 2024-04-10T20:38:15Z |
| format | Article |
| id | doaj.art-5b22908a190a4815a277839de3532a99 |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-04-10T20:38:15Z |
| publishDate | 2022-02-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-5b22908a190a4815a277839de3532a992023-01-24T20:29:01ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592022-02-0143210.5433/1679-0359.2022v43n2p841Effect of quercetin or butylated hydroxytoluene on cooled or frozen-thawed ram sperm qualityLucas Emanuel Ferreira Canuto0Lorenzo Garrido Teixeira Martini Segabinazzi1Endrigo Adonis Braga de Araújo2Luis Fernando Mercês Chaves Silva3Sidnei Nunes de Oliveira4Felipe Morales Dalanezi5José Antonio Dell'Aqua Junior6Frederico Ozanam Papa7Eunice Oba8Universidade Estadual Paulista Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista Faculdade de Medicina Veterinária e ZootecniaCooling and freezing processes cause physical and chemical damage to sperm by cold shock and oxidative stress. This study aimed to evaluate the effect of two antioxidants on sperm parameters of cooled and frozen-thawed ram semen diluted in an egg yolk-based extender. Semen was collected from 30 rams and processed in two consecutive experiments to test the inclusion of different concentrations of quercetin and butylated hydroxytoluene (BHT) in an egg yolk-based semen extender. Dimethyl sulfoxide (DMSO) was added as a solvent to the semen extender in a ratio of 1 mL DMSO for 90 mg of quercetin and 1 mL DMSO for 880 mg of BHT. After collection, semen was diluted at 200 × 106 motile sperm/mL (control) and split into different groups in each experiment. In experiment 1, semen was diluted with the extender containing quercetin (Q5, 5 µg/mL; Q10, 10 ug/mL; Q15, 15 ug/mL) or DMSO alone (DMSO1, 0.055 uL DMSO per mL; DMSO2, 0.165 uL DMSO per mL). In experiment 2, semen was diluted with the extender with BHT (BHT1, 0.5 ug/mL; BHT2, 1 ug/mL; BHT3, 1.5 ug/mL) or DMSO alone (DMSO3, 0.375 uL DMSO per mL; DMSO4, 1.125 uL DMSO per mL). After dilution, the semen was divided into two aliquots. Treated ram sperm samples were also subjected to different storage methods. The first set of samples was cooled at 5 °C for 24 h, whereas the second set of samples was frozen-thawed. Sperm motility parameters and plasma membrane integrity (PMI) were evaluated immediately after dilution (0h) and 24 h after cooling and in the frozen-thawed samples via computer-assisted sperm analysis and epifluorescence microscopy, respectively. The inclusion of quercetin or BHT did not affect sperm motility parameters or PMI of fresh, cooled, or frozen-thawed sperm in this study (P < 0.05). However, further studies are needed to test the effects of these antioxidants on the fertility of cryopreserved ram semen.https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44068AntioxidantCryopreservationOxidative damageLipid peroxidationSmall ruminantsSheep. |
| spellingShingle | Lucas Emanuel Ferreira Canuto Lorenzo Garrido Teixeira Martini Segabinazzi Endrigo Adonis Braga de Araújo Luis Fernando Mercês Chaves Silva Sidnei Nunes de Oliveira Felipe Morales Dalanezi José Antonio Dell'Aqua Junior Frederico Ozanam Papa Eunice Oba Effect of quercetin or butylated hydroxytoluene on cooled or frozen-thawed ram sperm quality Semina: Ciências Agrárias Antioxidant Cryopreservation Oxidative damage Lipid peroxidation Small ruminants Sheep. |
| title | Effect of quercetin or butylated hydroxytoluene on cooled or frozen-thawed ram sperm quality |
| title_full | Effect of quercetin or butylated hydroxytoluene on cooled or frozen-thawed ram sperm quality |
| title_fullStr | Effect of quercetin or butylated hydroxytoluene on cooled or frozen-thawed ram sperm quality |
| title_full_unstemmed | Effect of quercetin or butylated hydroxytoluene on cooled or frozen-thawed ram sperm quality |
| title_short | Effect of quercetin or butylated hydroxytoluene on cooled or frozen-thawed ram sperm quality |
| title_sort | effect of quercetin or butylated hydroxytoluene on cooled or frozen thawed ram sperm quality |
| topic | Antioxidant Cryopreservation Oxidative damage Lipid peroxidation Small ruminants Sheep. |
| url | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44068 |
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