Anticancer Podophyllotoxin Recovery from Juniper Leaves at Atmospheric and High Pressure Using Eco-Friendly Solvents
Podophyllotoxin (PPT) is a precursor for the synthesis of drugs against cancer and other diseases. The present sources of PPT (<i>Sinopodophyllum hexandrum</i> and <i>Podophyllum peltatum</i>) are endangered species, with PPT production highly dependent on their growing condi...
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MDPI AG
2023-03-01
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| Online Access: | https://www.mdpi.com/2223-7747/12/7/1526 |
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| author | Diana Ivanova Paraskev Nedialkov Alexander Tashev Zlatina Kokanova-Nedialkova Marta Olech Renata Nowak Stanislava Boyadzhieva George Angelov Dragomir Yankov |
| author_facet | Diana Ivanova Paraskev Nedialkov Alexander Tashev Zlatina Kokanova-Nedialkova Marta Olech Renata Nowak Stanislava Boyadzhieva George Angelov Dragomir Yankov |
| author_sort | Diana Ivanova |
| collection | DOAJ |
| description | Podophyllotoxin (PPT) is a precursor for the synthesis of drugs against cancer and other diseases. The present sources of PPT (<i>Sinopodophyllum hexandrum</i> and <i>Podophyllum peltatum</i>) are endangered species, with PPT production highly dependent on their growing conditions. In connection with the identification of new sources of PPT, the present study aimed to recover PPT from <i>Juniperus virginiana</i> leaves via atmospheric or high pressure extraction methods with a focus on using eco-friendly solvents. PPT quantification was determined by UHPLC/HRMS/MS. A thorough study of conventional extraction was carried out to reveal the optimal conditions (solvent ethyl acetate at room temperature and a duration of 1 h) for maximizing the PPT recovery (about 30 mg/g of dry extract and 3 mg/g of dry initial plant material). Peleg’s equation was applied for process kinetics modeling. The best PPT content in the final dry extract (42–45 mg/g of dry extract) was obtained by high pressure methods under supercritical (scCO<sub>2</sub> with ethanol or ethyl acetate, 30 MPa, 50 °C and 100 min) or accelerated solvent extraction conditions (solvent ethyl acetate, 10.35 MPa, 20 °C and 3 cycles for 15 min). Seasonal stability and storage stability of the raw material were also determined. The present results have potential applications in the pharmacy for the delivery of PPT from juniper leaves. |
| first_indexed | 2024-03-11T05:27:41Z |
| format | Article |
| id | doaj.art-5b23fbf05cf74df1ad346f72a2bb6d6f |
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| language | English |
| last_indexed | 2024-03-11T05:27:41Z |
| publishDate | 2023-03-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-5b23fbf05cf74df1ad346f72a2bb6d6f2023-11-17T17:23:51ZengMDPI AGPlants2223-77472023-03-01127152610.3390/plants12071526Anticancer Podophyllotoxin Recovery from Juniper Leaves at Atmospheric and High Pressure Using Eco-Friendly SolventsDiana Ivanova0Paraskev Nedialkov1Alexander Tashev2Zlatina Kokanova-Nedialkova3Marta Olech4Renata Nowak5Stanislava Boyadzhieva6George Angelov7Dragomir Yankov8Institute of Chemical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaDepartment of Pharmacognosy, Faculty of Pharmacy, Medical University, 1000 Sofia, BulgariaDepartment of Dendrology, University of Forestry, 1756 Sofia, BulgariaDepartment of Pharmacognosy, Faculty of Pharmacy, Medical University, 1000 Sofia, BulgariaDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Lublin, 20-059 Lublin, PolandDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Lublin, 20-059 Lublin, PolandInstitute of Chemical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaInstitute of Chemical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaInstitute of Chemical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaPodophyllotoxin (PPT) is a precursor for the synthesis of drugs against cancer and other diseases. The present sources of PPT (<i>Sinopodophyllum hexandrum</i> and <i>Podophyllum peltatum</i>) are endangered species, with PPT production highly dependent on their growing conditions. In connection with the identification of new sources of PPT, the present study aimed to recover PPT from <i>Juniperus virginiana</i> leaves via atmospheric or high pressure extraction methods with a focus on using eco-friendly solvents. PPT quantification was determined by UHPLC/HRMS/MS. A thorough study of conventional extraction was carried out to reveal the optimal conditions (solvent ethyl acetate at room temperature and a duration of 1 h) for maximizing the PPT recovery (about 30 mg/g of dry extract and 3 mg/g of dry initial plant material). Peleg’s equation was applied for process kinetics modeling. The best PPT content in the final dry extract (42–45 mg/g of dry extract) was obtained by high pressure methods under supercritical (scCO<sub>2</sub> with ethanol or ethyl acetate, 30 MPa, 50 °C and 100 min) or accelerated solvent extraction conditions (solvent ethyl acetate, 10.35 MPa, 20 °C and 3 cycles for 15 min). Seasonal stability and storage stability of the raw material were also determined. The present results have potential applications in the pharmacy for the delivery of PPT from juniper leaves.https://www.mdpi.com/2223-7747/12/7/1526<i>Juniperus virginiana</i> L.podophyllotoxinextraction optimizationsupercritical fluid extractioneco-friendly solventsUHPLC/HRMS/MS |
| spellingShingle | Diana Ivanova Paraskev Nedialkov Alexander Tashev Zlatina Kokanova-Nedialkova Marta Olech Renata Nowak Stanislava Boyadzhieva George Angelov Dragomir Yankov Anticancer Podophyllotoxin Recovery from Juniper Leaves at Atmospheric and High Pressure Using Eco-Friendly Solvents Plants <i>Juniperus virginiana</i> L. podophyllotoxin extraction optimization supercritical fluid extraction eco-friendly solvents UHPLC/HRMS/MS |
| title | Anticancer Podophyllotoxin Recovery from Juniper Leaves at Atmospheric and High Pressure Using Eco-Friendly Solvents |
| title_full | Anticancer Podophyllotoxin Recovery from Juniper Leaves at Atmospheric and High Pressure Using Eco-Friendly Solvents |
| title_fullStr | Anticancer Podophyllotoxin Recovery from Juniper Leaves at Atmospheric and High Pressure Using Eco-Friendly Solvents |
| title_full_unstemmed | Anticancer Podophyllotoxin Recovery from Juniper Leaves at Atmospheric and High Pressure Using Eco-Friendly Solvents |
| title_short | Anticancer Podophyllotoxin Recovery from Juniper Leaves at Atmospheric and High Pressure Using Eco-Friendly Solvents |
| title_sort | anticancer podophyllotoxin recovery from juniper leaves at atmospheric and high pressure using eco friendly solvents |
| topic | <i>Juniperus virginiana</i> L. podophyllotoxin extraction optimization supercritical fluid extraction eco-friendly solvents UHPLC/HRMS/MS |
| url | https://www.mdpi.com/2223-7747/12/7/1526 |
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