Basal cell markers:34BE12 and p63, improving detection of basal cells in atypical prostatic lesions
Background: The diagnosis of prostatic pathology may be of challenging , as some difficult and suspected, atypical cases may lack basal cell layer by routine H&E sections . Antibodies against 34BE12(HMW-CK) and p63 aid the diagnosis of such cases , to distinguish benign from malignant pr...
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| Format: | Article |
| Language: | English |
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University of Baghdad/ Al-Kindy College of Medicine
2019-04-01
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| Series: | مجله كليه طب الكندي |
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| Online Access: | https://jkmc.uobaghdad.edu.iq/index.php/MEDICAL/article/view/95 |
| _version_ | 1798002011293089792 |
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| author | Inas A. Rasheed |
| author_facet | Inas A. Rasheed |
| author_sort | Inas A. Rasheed |
| collection | DOAJ |
| description |
Background: The diagnosis of prostatic pathology may be of challenging , as some difficult and suspected, atypical cases may lack basal cell layer by routine H&E sections . Antibodies against 34BE12(HMW-CK) and p63 aid the diagnosis of such cases , to distinguish benign from malignant prostatic lesions.
Objective: to identify basal cells in atypical prostatic lesions ,and distinguish benign from malignant prostatic lesions.
Type of the study: A retro-spective study.
Methods: 115cases of paraffin embedded prostatic tissue blocks ,diagnosed as : 76 cases were benign prostatic hyperplasia( BPH) , 9 cases were high grade –prostatic intraepithelial neoplasia (HG-PIN) , and 30 cases were prostatic carcinoma(PCa) .Sections from each blocks were prepared for immunostaining with 34BE12 and p63.
Results : basal cells were detected in cases of BPH , and HG-PIN , and absent in all cases of prostatic carcinoma ,using basal cell markers . Negative benign glands(>2) were found in 71.6% and 38.2% for BPH and 57.1% and 55.6% for HG-PIN immunostained with high molecular weight cytokeratin (34BE12) and p63 , respectively, and significantly reduced to 9.0% and 11.1% for BPH and HG-PIN, respectively with combined using of both markers .Conclusion : Combination of both basal cell markers (34BE12 , p63) improving basal cell detection in atypical ,suspected prostatic lesions and distinguish benign from malignant lesions.
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| first_indexed | 2024-04-11T11:46:32Z |
| format | Article |
| id | doaj.art-5b243096734a4d4fb07736a7331585ed |
| institution | Directory Open Access Journal |
| issn | 1810-9543 2521-4365 |
| language | English |
| last_indexed | 2024-04-11T11:46:32Z |
| publishDate | 2019-04-01 |
| publisher | University of Baghdad/ Al-Kindy College of Medicine |
| record_format | Article |
| series | مجله كليه طب الكندي |
| spelling | doaj.art-5b243096734a4d4fb07736a7331585ed2022-12-22T04:25:34ZengUniversity of Baghdad/ Al-Kindy College of Medicineمجله كليه طب الكندي1810-95432521-43652019-04-0113210.47723/kcmj.v13i2.95Basal cell markers:34BE12 and p63, improving detection of basal cells in atypical prostatic lesionsInas A. Rasheed Background: The diagnosis of prostatic pathology may be of challenging , as some difficult and suspected, atypical cases may lack basal cell layer by routine H&E sections . Antibodies against 34BE12(HMW-CK) and p63 aid the diagnosis of such cases , to distinguish benign from malignant prostatic lesions. Objective: to identify basal cells in atypical prostatic lesions ,and distinguish benign from malignant prostatic lesions. Type of the study: A retro-spective study. Methods: 115cases of paraffin embedded prostatic tissue blocks ,diagnosed as : 76 cases were benign prostatic hyperplasia( BPH) , 9 cases were high grade –prostatic intraepithelial neoplasia (HG-PIN) , and 30 cases were prostatic carcinoma(PCa) .Sections from each blocks were prepared for immunostaining with 34BE12 and p63. Results : basal cells were detected in cases of BPH , and HG-PIN , and absent in all cases of prostatic carcinoma ,using basal cell markers . Negative benign glands(>2) were found in 71.6% and 38.2% for BPH and 57.1% and 55.6% for HG-PIN immunostained with high molecular weight cytokeratin (34BE12) and p63 , respectively, and significantly reduced to 9.0% and 11.1% for BPH and HG-PIN, respectively with combined using of both markers .Conclusion : Combination of both basal cell markers (34BE12 , p63) improving basal cell detection in atypical ,suspected prostatic lesions and distinguish benign from malignant lesions. https://jkmc.uobaghdad.edu.iq/index.php/MEDICAL/article/view/95BPH ,HG-PIN , prostatic carcinoma , basal cell markers, 34BE12 , HMW-CK , p63 |
| spellingShingle | Inas A. Rasheed Basal cell markers:34BE12 and p63, improving detection of basal cells in atypical prostatic lesions مجله كليه طب الكندي BPH ,HG-PIN , prostatic carcinoma , basal cell markers, 34BE12 , HMW-CK , p63 |
| title | Basal cell markers:34BE12 and p63, improving detection of basal cells in atypical prostatic lesions |
| title_full | Basal cell markers:34BE12 and p63, improving detection of basal cells in atypical prostatic lesions |
| title_fullStr | Basal cell markers:34BE12 and p63, improving detection of basal cells in atypical prostatic lesions |
| title_full_unstemmed | Basal cell markers:34BE12 and p63, improving detection of basal cells in atypical prostatic lesions |
| title_short | Basal cell markers:34BE12 and p63, improving detection of basal cells in atypical prostatic lesions |
| title_sort | basal cell markers 34be12 and p63 improving detection of basal cells in atypical prostatic lesions |
| topic | BPH ,HG-PIN , prostatic carcinoma , basal cell markers, 34BE12 , HMW-CK , p63 |
| url | https://jkmc.uobaghdad.edu.iq/index.php/MEDICAL/article/view/95 |
| work_keys_str_mv | AT inasarasheed basalcellmarkers34be12andp63improvingdetectionofbasalcellsinatypicalprostaticlesions |