Waste Cassava Tuber Fibers as an Immobilization Carrier of Saccharomyces cerevisiae for Ethanol Production

Waste cassava tuber fibers (wCTF), derived from the ethanolic fermentation of cassava tubers, have potential use as anatural adsorption immobilization carrier. Ethanol fermentation was conducted using 15% (w/v) glucose-containing mediumat 40 °C for 48 h by Saccharomyces cerevisiae G6-2-2 (1.3 x 1010...

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Main Authors: Sineenath Kunthiphun, Pongphannee Phumikhet, Vasana Tolieng, Somboon Tanasupawat, Ancharida Akaracharanya
Format: Article
Language:English
Published: North Carolina State University 2016-11-01
Series:BioResources
Subjects:
Online Access:http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_12_1_157_Kunthiphun_Waste_Cassava_Tuber_Fibers
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author Sineenath Kunthiphun
Pongphannee Phumikhet
Vasana Tolieng
Somboon Tanasupawat
Ancharida Akaracharanya
author_facet Sineenath Kunthiphun
Pongphannee Phumikhet
Vasana Tolieng
Somboon Tanasupawat
Ancharida Akaracharanya
author_sort Sineenath Kunthiphun
collection DOAJ
description Waste cassava tuber fibers (wCTF), derived from the ethanolic fermentation of cassava tubers, have potential use as anatural adsorption immobilization carrier. Ethanol fermentation was conducted using 15% (w/v) glucose-containing mediumat 40 °C for 48 h by Saccharomyces cerevisiae G6-2-2 (1.3 x 1010cells). Ethanol concentration produced by free, wCTF (1.2 g dry weight) adsorbed, wCTFadsorbed-calcium alginate entrapped,and calcium alginate entrapped cellswere 42.10 ± 0.61, 67.35 ± 0.53, 52.10 ± 0.40, and 46.45 ± 0.18 g/L (0.34, 0.45, 0.35, and 0.31 g ethanol/g reducing sugar), respectively. The wCTF adsorbed cells produced a maximum ethanol yield of 82.15 ± 0.48 g/L (0.43 g ethanol/g total sugar) from molasses (20% w/v initial total sugar) after 48 h, compared to 74 g/L to 76 g/L and 48 h to 100 h for the free suspension cells. The increase in ethanol produced by the wCTF adsorbed cells compared to free cells reflected that the cells were protected from environmental stresses and received amino nitrogen from the wCTF that supported growth and ethanol tolerance.
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spelling doaj.art-5b2467bca6dc43118b48a128349098b72022-12-21T19:55:06ZengNorth Carolina State UniversityBioResources1930-21261930-21262016-11-0112115716710.15376/biores.12.1.157-167Waste Cassava Tuber Fibers as an Immobilization Carrier of Saccharomyces cerevisiae for Ethanol ProductionSineenath Kunthiphun0Pongphannee Phumikhet1Vasana Tolieng2Somboon Tanasupawat3Ancharida Akaracharanya4Chulalongkorn University; ThailandChulalongkorn University; ThailandChulalongkorn University; ThailandChulalongkorn University; ThailandChulalongkorn University; ThailandWaste cassava tuber fibers (wCTF), derived from the ethanolic fermentation of cassava tubers, have potential use as anatural adsorption immobilization carrier. Ethanol fermentation was conducted using 15% (w/v) glucose-containing mediumat 40 °C for 48 h by Saccharomyces cerevisiae G6-2-2 (1.3 x 1010cells). Ethanol concentration produced by free, wCTF (1.2 g dry weight) adsorbed, wCTFadsorbed-calcium alginate entrapped,and calcium alginate entrapped cellswere 42.10 ± 0.61, 67.35 ± 0.53, 52.10 ± 0.40, and 46.45 ± 0.18 g/L (0.34, 0.45, 0.35, and 0.31 g ethanol/g reducing sugar), respectively. The wCTF adsorbed cells produced a maximum ethanol yield of 82.15 ± 0.48 g/L (0.43 g ethanol/g total sugar) from molasses (20% w/v initial total sugar) after 48 h, compared to 74 g/L to 76 g/L and 48 h to 100 h for the free suspension cells. The increase in ethanol produced by the wCTF adsorbed cells compared to free cells reflected that the cells were protected from environmental stresses and received amino nitrogen from the wCTF that supported growth and ethanol tolerance.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_12_1_157_Kunthiphun_Waste_Cassava_Tuber_FibersWaste cassava tuber fiber; Natural immobilization carrier; Immobilization Ethanol
spellingShingle Sineenath Kunthiphun
Pongphannee Phumikhet
Vasana Tolieng
Somboon Tanasupawat
Ancharida Akaracharanya
Waste Cassava Tuber Fibers as an Immobilization Carrier of Saccharomyces cerevisiae for Ethanol Production
BioResources
Waste cassava tuber fiber; Natural immobilization carrier; Immobilization Ethanol
title Waste Cassava Tuber Fibers as an Immobilization Carrier of Saccharomyces cerevisiae for Ethanol Production
title_full Waste Cassava Tuber Fibers as an Immobilization Carrier of Saccharomyces cerevisiae for Ethanol Production
title_fullStr Waste Cassava Tuber Fibers as an Immobilization Carrier of Saccharomyces cerevisiae for Ethanol Production
title_full_unstemmed Waste Cassava Tuber Fibers as an Immobilization Carrier of Saccharomyces cerevisiae for Ethanol Production
title_short Waste Cassava Tuber Fibers as an Immobilization Carrier of Saccharomyces cerevisiae for Ethanol Production
title_sort waste cassava tuber fibers as an immobilization carrier of saccharomyces cerevisiae for ethanol production
topic Waste cassava tuber fiber; Natural immobilization carrier; Immobilization Ethanol
url http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_12_1_157_Kunthiphun_Waste_Cassava_Tuber_Fibers
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