Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels
Neurons are a diverse cell type exhibiting hugely different morphologies and neurotransmitter specifications. Their distinctive phenotypes are established during differentiation from pluripotent precursor cells. The signalling pathways that specify the lineage down which neuronal precursor cells dif...
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| Format: | Article |
| Language: | English |
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BMC
2008-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200008 |
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| author | PAUL CUDDON MARTIN D BOOTMAN GILLIAN R RICHARDS ALISON J SMITH PETER B SIMPSON H LLEWELYN RODERICK |
| author_facet | PAUL CUDDON MARTIN D BOOTMAN GILLIAN R RICHARDS ALISON J SMITH PETER B SIMPSON H LLEWELYN RODERICK |
| author_sort | PAUL CUDDON |
| collection | DOAJ |
| description | Neurons are a diverse cell type exhibiting hugely different morphologies and neurotransmitter specifications. Their distinctive phenotypes are established during differentiation from pluripotent precursor cells. The signalling pathways that specify the lineage down which neuronal precursor cells differentiate remain to be fully elucidated. Among the many signáis that impinge on the differentiation of neuronal cells, cytosolic calcium (Ca2+) has an important role. However, little is known about the nature of the Ca2+ signáis involved in fate choice in neuronal precursor cells, or their sources. In this study, we show that activation of either muscarinic or platelet-derived growth factor (PDGF) receptors induces a biphasic increase in cytosolic Ca2+ that consists of reléase from intracellular stores followed by sustained entry across the plasma membrane. For both agonists, the prolonged Ca2+ entry occurred via a store-operated pathway that was pharmacologically indistinguishable from Ca2+ entry initiated by thapsigargin. However, muscarinic receptor-activated Ca2+ entry was inhibited by siRNA-mediated knockdown of TRPC6, whereas Ca2+ entry evoked by PDGF was not. These data provide evidence for agonist-specific activation of molecularly distinct store-operated Ca2+ entry pathways, and raise the possibility of privileged communication between these Ca2+ entry pathways and downstream processes. |
| first_indexed | 2024-12-13T14:41:04Z |
| format | Article |
| id | doaj.art-5b2950a7db644d21b4067656dd054e83 |
| institution | Directory Open Access Journal |
| issn | 0716-9760 0717-6287 |
| language | English |
| last_indexed | 2024-12-13T14:41:04Z |
| publishDate | 2008-01-01 |
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| spelling | doaj.art-5b2950a7db644d21b4067656dd054e832022-12-21T23:41:36ZengBMCBiological Research0716-97600717-62872008-01-01412183195Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channelsPAUL CUDDONMARTIN D BOOTMANGILLIAN R RICHARDSALISON J SMITHPETER B SIMPSONH LLEWELYN RODERICKNeurons are a diverse cell type exhibiting hugely different morphologies and neurotransmitter specifications. Their distinctive phenotypes are established during differentiation from pluripotent precursor cells. The signalling pathways that specify the lineage down which neuronal precursor cells differentiate remain to be fully elucidated. Among the many signáis that impinge on the differentiation of neuronal cells, cytosolic calcium (Ca2+) has an important role. However, little is known about the nature of the Ca2+ signáis involved in fate choice in neuronal precursor cells, or their sources. In this study, we show that activation of either muscarinic or platelet-derived growth factor (PDGF) receptors induces a biphasic increase in cytosolic Ca2+ that consists of reléase from intracellular stores followed by sustained entry across the plasma membrane. For both agonists, the prolonged Ca2+ entry occurred via a store-operated pathway that was pharmacologically indistinguishable from Ca2+ entry initiated by thapsigargin. However, muscarinic receptor-activated Ca2+ entry was inhibited by siRNA-mediated knockdown of TRPC6, whereas Ca2+ entry evoked by PDGF was not. These data provide evidence for agonist-specific activation of molecularly distinct store-operated Ca2+ entry pathways, and raise the possibility of privileged communication between these Ca2+ entry pathways and downstream processes.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200008Ca2+PDGFmethacholineTRPC6SOCEhuman neurosphere-derived cells |
| spellingShingle | PAUL CUDDON MARTIN D BOOTMAN GILLIAN R RICHARDS ALISON J SMITH PETER B SIMPSON H LLEWELYN RODERICK Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels Biological Research Ca2+ PDGF methacholine TRPC6 SOCE human neurosphere-derived cells |
| title | Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels |
| title_full | Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels |
| title_fullStr | Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels |
| title_full_unstemmed | Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels |
| title_short | Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels |
| title_sort | methacholine and pdgf activate store operated calcium entry in neuronal precursor cells via distinct calcium entry channels |
| topic | Ca2+ PDGF methacholine TRPC6 SOCE human neurosphere-derived cells |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200008 |
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