In vitro seed germination and seedling performance of Hibiscus coddii subsp. barnardii
Hibiscus coddii subsp. barnardii is an endemic South African plant species with ornamental potential. The limited plant availability in nature and restrictions on the collection of living plant material (cuttings) for cultivation purposes, necessitated the development of a protocol for in vitro seed...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Floricultura e Plantas Ornamentais
2020-09-01
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| Series: | Ornamental Horticulture |
| Subjects: | |
| Online Access: | https://ornamentalhorticulture.emnuvens.com.br/rbho/article/view/2191 |
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| author | Helena Jacoba Du Plessis Roumiana Vassileva Nikolova Riana Kleynhans Bronwyn Anne Egan |
| author_facet | Helena Jacoba Du Plessis Roumiana Vassileva Nikolova Riana Kleynhans Bronwyn Anne Egan |
| author_sort | Helena Jacoba Du Plessis |
| collection | DOAJ |
| description | Hibiscus coddii subsp. barnardii is an endemic South African plant species with ornamental potential. The limited plant availability in nature and restrictions on the collection of living plant material (cuttings) for cultivation purposes, necessitated the development of a protocol for in vitro seed propagation to ensure all year round ex vitro establishment of plants. The effect of culture medium on seed germination and seedling performance was, therefore, studied. Various types of sterile seed cultures, namely moist filter paper bridges, five different strengths (¼, ½, ¾, full, 1¼) of PGR-free Murashige and Skoog medium (MS) and solid Gelrite® medium (control) were investigated. High germination percentages (85–98%) were found in all three seed cultures, although seeds germinated on filter paper, Gelrite® and lower strengths (¼, ½, ¾) of MS medium had shorter mean germination times (1.6–2.8 days) than those germinated on full and 1¼ strengths (3.5–4 days) of MS medium. Furthermore, the growth performance of seedlings varied with the strength of MS medium and was best supported on full strength MS medium, whereas ¼ and ½ strength MS media and Gelrite® cultures were detrimental for seedling growth. |
| first_indexed | 2024-03-13T03:48:47Z |
| format | Article |
| id | doaj.art-5b2c2cf43998402984ace84075d18bc9 |
| institution | Directory Open Access Journal |
| issn | 2447-536X |
| language | English |
| last_indexed | 2024-03-13T03:48:47Z |
| publishDate | 2020-09-01 |
| publisher | Sociedade Brasileira de Floricultura e Plantas Ornamentais |
| record_format | Article |
| series | Ornamental Horticulture |
| spelling | doaj.art-5b2c2cf43998402984ace84075d18bc92023-06-22T20:34:33ZengSociedade Brasileira de Floricultura e Plantas OrnamentaisOrnamental Horticulture2447-536X2020-09-0126410.1590/2447-536X.v26i4.21912141In vitro seed germination and seedling performance of Hibiscus coddii subsp. barnardiiHelena Jacoba Du Plessis0Roumiana Vassileva Nikolova1Riana Kleynhans2Bronwyn Anne Egan3University of LimpopoUniversity of LimpopoTshwane University of TechnologyUniversity of LimpopoHibiscus coddii subsp. barnardii is an endemic South African plant species with ornamental potential. The limited plant availability in nature and restrictions on the collection of living plant material (cuttings) for cultivation purposes, necessitated the development of a protocol for in vitro seed propagation to ensure all year round ex vitro establishment of plants. The effect of culture medium on seed germination and seedling performance was, therefore, studied. Various types of sterile seed cultures, namely moist filter paper bridges, five different strengths (¼, ½, ¾, full, 1¼) of PGR-free Murashige and Skoog medium (MS) and solid Gelrite® medium (control) were investigated. High germination percentages (85–98%) were found in all three seed cultures, although seeds germinated on filter paper, Gelrite® and lower strengths (¼, ½, ¾) of MS medium had shorter mean germination times (1.6–2.8 days) than those germinated on full and 1¼ strengths (3.5–4 days) of MS medium. Furthermore, the growth performance of seedlings varied with the strength of MS medium and was best supported on full strength MS medium, whereas ¼ and ½ strength MS media and Gelrite® cultures were detrimental for seedling growth. https://ornamentalhorticulture.emnuvens.com.br/rbho/article/view/2191Hibiscus coddii subsp. barnardiiIn vitro seed cultureFilter paper bridgesGelrite® mediumMurashige and Skoog mediumIn vitro grown seedlings |
| spellingShingle | Helena Jacoba Du Plessis Roumiana Vassileva Nikolova Riana Kleynhans Bronwyn Anne Egan In vitro seed germination and seedling performance of Hibiscus coddii subsp. barnardii Ornamental Horticulture Hibiscus coddii subsp. barnardii In vitro seed culture Filter paper bridges Gelrite® medium Murashige and Skoog medium In vitro grown seedlings |
| title | In vitro seed germination and seedling performance of Hibiscus coddii subsp. barnardii |
| title_full | In vitro seed germination and seedling performance of Hibiscus coddii subsp. barnardii |
| title_fullStr | In vitro seed germination and seedling performance of Hibiscus coddii subsp. barnardii |
| title_full_unstemmed | In vitro seed germination and seedling performance of Hibiscus coddii subsp. barnardii |
| title_short | In vitro seed germination and seedling performance of Hibiscus coddii subsp. barnardii |
| title_sort | in vitro seed germination and seedling performance of hibiscus coddii subsp barnardii |
| topic | Hibiscus coddii subsp. barnardii In vitro seed culture Filter paper bridges Gelrite® medium Murashige and Skoog medium In vitro grown seedlings |
| url | https://ornamentalhorticulture.emnuvens.com.br/rbho/article/view/2191 |
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