Using click chemistry to study microbial ecology and evolution
Abstract Technological advances have largely driven the revolution in our understanding of the structure and function of microbial communities. Culturing, long the primary tool to probe microbial life, was supplanted by sequencing and other -omics approaches, which allowed detailed quantitative insi...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2023-01-01
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| Series: | ISME Communications |
| Online Access: | https://doi.org/10.1038/s43705-022-00205-5 |
| _version_ | 1797226287625928704 |
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| author | Sander van Kasteren Daniel E. Rozen |
| author_facet | Sander van Kasteren Daniel E. Rozen |
| author_sort | Sander van Kasteren |
| collection | DOAJ |
| description | Abstract Technological advances have largely driven the revolution in our understanding of the structure and function of microbial communities. Culturing, long the primary tool to probe microbial life, was supplanted by sequencing and other -omics approaches, which allowed detailed quantitative insights into species composition, metabolic potential, transcriptional activity, secretory responses and more. Although the ability to characterize “who’s there” has never been easier or cheaper, it remains technically challenging and expensive to understand what the diverse species and strains that comprise microbial communities are doing in situ, and how these behaviors change through time. Our aim in this brief review is to introduce a developing toolkit based on click chemistry that can accelerate and reduce the expense of functional analyses of the ecology and evolution of microbial communities. After first outlining the history of technological development in this field, we will discuss key applications to date using diverse labels, including BONCAT, and then end with a selective (biased) view of areas where click-chemistry and BONCAT-based approaches stand to have a significant impact on our understanding of microbial communities. |
| first_indexed | 2024-04-24T14:22:31Z |
| format | Article |
| id | doaj.art-5b2d83da72124c2baeaf48ffd53799a4 |
| institution | Directory Open Access Journal |
| issn | 2730-6151 |
| language | English |
| last_indexed | 2024-04-24T14:22:31Z |
| publishDate | 2023-01-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | ISME Communications |
| spelling | doaj.art-5b2d83da72124c2baeaf48ffd53799a42024-04-03T06:16:57ZengOxford University PressISME Communications2730-61512023-01-01311910.1038/s43705-022-00205-5Using click chemistry to study microbial ecology and evolutionSander van Kasteren0Daniel E. Rozen1Leiden Institute of Chemistry and The Institute of Chemical Immunology, Leiden UniversityInstitute of Biology, Leiden UniversityAbstract Technological advances have largely driven the revolution in our understanding of the structure and function of microbial communities. Culturing, long the primary tool to probe microbial life, was supplanted by sequencing and other -omics approaches, which allowed detailed quantitative insights into species composition, metabolic potential, transcriptional activity, secretory responses and more. Although the ability to characterize “who’s there” has never been easier or cheaper, it remains technically challenging and expensive to understand what the diverse species and strains that comprise microbial communities are doing in situ, and how these behaviors change through time. Our aim in this brief review is to introduce a developing toolkit based on click chemistry that can accelerate and reduce the expense of functional analyses of the ecology and evolution of microbial communities. After first outlining the history of technological development in this field, we will discuss key applications to date using diverse labels, including BONCAT, and then end with a selective (biased) view of areas where click-chemistry and BONCAT-based approaches stand to have a significant impact on our understanding of microbial communities.https://doi.org/10.1038/s43705-022-00205-5 |
| spellingShingle | Sander van Kasteren Daniel E. Rozen Using click chemistry to study microbial ecology and evolution ISME Communications |
| title | Using click chemistry to study microbial ecology and evolution |
| title_full | Using click chemistry to study microbial ecology and evolution |
| title_fullStr | Using click chemistry to study microbial ecology and evolution |
| title_full_unstemmed | Using click chemistry to study microbial ecology and evolution |
| title_short | Using click chemistry to study microbial ecology and evolution |
| title_sort | using click chemistry to study microbial ecology and evolution |
| url | https://doi.org/10.1038/s43705-022-00205-5 |
| work_keys_str_mv | AT sandervankasteren usingclickchemistrytostudymicrobialecologyandevolution AT danielerozen usingclickchemistrytostudymicrobialecologyandevolution |