Characterization of Nanoparticles Using DSPE-PEG2000 and Soluplus
The aim of this study was to evaluate the characterized hydration method to prepare nanoparticles using Soluplus, a block copolymer with amphipathic properties, and distearoyl phosphatidyl ethanolamine (DSPE)-PEG2000 owing to particle size distribution, zeta potential, particle stability, and transm...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Colloids and Interfaces |
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| Online Access: | https://www.mdpi.com/2504-5377/4/3/28 |
| _version_ | 1797562171472740352 |
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| author | Rina Takayama Yutaka Inoue Isamu Murata Ikuo Kanamoto |
| author_facet | Rina Takayama Yutaka Inoue Isamu Murata Ikuo Kanamoto |
| author_sort | Rina Takayama |
| collection | DOAJ |
| description | The aim of this study was to evaluate the characterized hydration method to prepare nanoparticles using Soluplus, a block copolymer with amphipathic properties, and distearoyl phosphatidyl ethanolamine (DSPE)-PEG2000 owing to particle size distribution, zeta potential, particle stability, and transmission electron microscopy (TEM) observed and <sup>31</sup>P-NMR spectra. The results showed that, in a suspension of DSPE-PEG2000 and Soluplus at a ratio of 1/1, the prepared microparticles were stable for five days in the dark and at 25 °C. It was also confirmed that the 1/1 suspension of DSPE-PEG2000/Soluplus was stable for five days under the same conditions with the magnesium chloride solution. TEM measurements confirmed the presence of micelle-like particles of 50 to 150 nm in the 1/1 ratio mix of DSPE-PEG2000/Soluplus. <sup>31</sup>P-NMR spectral data confirmed that DPSE-PEG2000/Soluplus at mixing ratio of 1/1 has a strong intermolecular with the phosphate group, indicated by the fact that the peak shift and the full width at half maximum were the largest compared with DSPE-PEG2000 with the intermolecular interaction. On the basis of the findings of this study, we conclude that microparticles can be formed using DSPE-PEG2000 and Soluplus via the hydration method, and that the optimum weight ratio of DSPE-PEG2000 to Soluplus is 1/1. |
| first_indexed | 2024-03-10T18:24:40Z |
| format | Article |
| id | doaj.art-5b2e010473d34b11aa399b123ae25d32 |
| institution | Directory Open Access Journal |
| issn | 2504-5377 |
| language | English |
| last_indexed | 2024-03-10T18:24:40Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Colloids and Interfaces |
| spelling | doaj.art-5b2e010473d34b11aa399b123ae25d322023-11-20T07:05:14ZengMDPI AGColloids and Interfaces2504-53772020-07-01432810.3390/colloids4030028Characterization of Nanoparticles Using DSPE-PEG2000 and SoluplusRina Takayama0Yutaka Inoue1Isamu Murata2Ikuo Kanamoto3Laboratory of Drug Safety Management, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, JapanLaboratory of Drug Safety Management, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, JapanLaboratory of Drug Safety Management, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, JapanLaboratory of Drug Safety Management, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, JapanThe aim of this study was to evaluate the characterized hydration method to prepare nanoparticles using Soluplus, a block copolymer with amphipathic properties, and distearoyl phosphatidyl ethanolamine (DSPE)-PEG2000 owing to particle size distribution, zeta potential, particle stability, and transmission electron microscopy (TEM) observed and <sup>31</sup>P-NMR spectra. The results showed that, in a suspension of DSPE-PEG2000 and Soluplus at a ratio of 1/1, the prepared microparticles were stable for five days in the dark and at 25 °C. It was also confirmed that the 1/1 suspension of DSPE-PEG2000/Soluplus was stable for five days under the same conditions with the magnesium chloride solution. TEM measurements confirmed the presence of micelle-like particles of 50 to 150 nm in the 1/1 ratio mix of DSPE-PEG2000/Soluplus. <sup>31</sup>P-NMR spectral data confirmed that DPSE-PEG2000/Soluplus at mixing ratio of 1/1 has a strong intermolecular with the phosphate group, indicated by the fact that the peak shift and the full width at half maximum were the largest compared with DSPE-PEG2000 with the intermolecular interaction. On the basis of the findings of this study, we conclude that microparticles can be formed using DSPE-PEG2000 and Soluplus via the hydration method, and that the optimum weight ratio of DSPE-PEG2000 to Soluplus is 1/1.https://www.mdpi.com/2504-5377/4/3/28nanoparticlesSoluplusDSPE-PEG2000intermolecular interaction<sup>31</sup>P-NMR |
| spellingShingle | Rina Takayama Yutaka Inoue Isamu Murata Ikuo Kanamoto Characterization of Nanoparticles Using DSPE-PEG2000 and Soluplus Colloids and Interfaces nanoparticles Soluplus DSPE-PEG2000 intermolecular interaction <sup>31</sup>P-NMR |
| title | Characterization of Nanoparticles Using DSPE-PEG2000 and Soluplus |
| title_full | Characterization of Nanoparticles Using DSPE-PEG2000 and Soluplus |
| title_fullStr | Characterization of Nanoparticles Using DSPE-PEG2000 and Soluplus |
| title_full_unstemmed | Characterization of Nanoparticles Using DSPE-PEG2000 and Soluplus |
| title_short | Characterization of Nanoparticles Using DSPE-PEG2000 and Soluplus |
| title_sort | characterization of nanoparticles using dspe peg2000 and soluplus |
| topic | nanoparticles Soluplus DSPE-PEG2000 intermolecular interaction <sup>31</sup>P-NMR |
| url | https://www.mdpi.com/2504-5377/4/3/28 |
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