Preliminary Comparison of Oral and Intestinal Human Microbiota in Patients with Colorectal Cancer: A Pilot Study
In this study Next-Generation Sequencing (NGS) was used to analyze and compare human microbiota from three different compartments, i.e., saliva, feces, and cancer tissue (CT), of a selected cohort of 10 Italian patients with colorectal cancer (CRC) vs. 10 healthy controls (saliva and feces). Further...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02699/full |
| _version_ | 1811314976627884032 |
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| author | Edda Russo Giovanni Bacci Carolina Chiellini Camilla Fagorzi Elena Niccolai Antonio Taddei Federica Ricci Maria N. Ringressi Rossella Borrelli Filippo Melli Manouela Miloeva Paolo Bechi Alessio Mengoni Renato Fani Amedeo Amedei Amedeo Amedei |
| author_facet | Edda Russo Giovanni Bacci Carolina Chiellini Camilla Fagorzi Elena Niccolai Antonio Taddei Federica Ricci Maria N. Ringressi Rossella Borrelli Filippo Melli Manouela Miloeva Paolo Bechi Alessio Mengoni Renato Fani Amedeo Amedei Amedeo Amedei |
| author_sort | Edda Russo |
| collection | DOAJ |
| description | In this study Next-Generation Sequencing (NGS) was used to analyze and compare human microbiota from three different compartments, i.e., saliva, feces, and cancer tissue (CT), of a selected cohort of 10 Italian patients with colorectal cancer (CRC) vs. 10 healthy controls (saliva and feces). Furthermore, the Fusobacterium nucleatum abundance in the same body site was investigated through real-time quantitative polymerase chain reaction (qPCR) to assess the association with CRC. Differences in bacterial composition, F. nucleatum abundance in healthy controls vs. CRC patients, and the association of F. nucleatum with clinical parameters were observed. Taxonomic analysis based on 16S rRNA gene, revealed the presence of three main bacterial phyla, which includes about 80% of reads: Firmicutes (39.18%), Bacteroidetes (30.36%), and Proteobacteria (10.65%). The results highlighted the presence of different bacterial compositions; in particular, the fecal samples of CRC patients seemed to be enriched with Bacteroidetes, whereas in the fecal samples of healthy controls Firmicutes were one of the major phyla detected though these differences were not statistically significant. The CT samples showed the highest alpha diversity values. These results emphasize a different taxonomic composition of feces from CRC compared to healthy controls. Despite the low number of samples included in the study, these results suggest the importance of microbiota in the CRC progression and could pave the way to the development of therapeutic interventions and novel microbial-related diagnostic tools in CRC patients. |
| first_indexed | 2024-04-13T11:22:07Z |
| format | Article |
| id | doaj.art-5b2fd3956b614c39b52cf9bff76d720c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T11:22:07Z |
| publishDate | 2018-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5b2fd3956b614c39b52cf9bff76d720c2022-12-22T02:48:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-01-01810.3389/fmicb.2017.02699284954Preliminary Comparison of Oral and Intestinal Human Microbiota in Patients with Colorectal Cancer: A Pilot StudyEdda Russo0Giovanni Bacci1Carolina Chiellini2Camilla Fagorzi3Elena Niccolai4Antonio Taddei5Federica Ricci6Maria N. Ringressi7Rossella Borrelli8Filippo Melli9Manouela Miloeva10Paolo Bechi11Alessio Mengoni12Renato Fani13Amedeo Amedei14Amedeo Amedei15Immunology, Department of Clinical and Experimental Medicine, University of Florence, Florence, ItalyDepartment of Biology, University of Florence, Florence, ItalyDepartment of Biology, University of Florence, Florence, ItalyDepartment of Biology, University of Florence, Florence, ItalyImmunology, Department of Clinical and Experimental Medicine, University of Florence, Florence, ItalyDepartment of Surgery and Translational Medicine, University of Florence, Florence, ItalyImmunology, Department of Clinical and Experimental Medicine, University of Florence, Florence, ItalyDepartment of Surgery and Translational Medicine, University of Florence, Florence, ItalyImmunology, Department of Clinical and Experimental Medicine, University of Florence, Florence, ItalyDepartment of Surgery and Translational Medicine, University of Florence, Florence, ItalyDepartment of Surgery and Translational Medicine, University of Florence, Florence, ItalyDepartment of Surgery and Translational Medicine, University of Florence, Florence, ItalyDepartment of Biology, University of Florence, Florence, ItalyDepartment of Biology, University of Florence, Florence, ItalyImmunology, Department of Clinical and Experimental Medicine, University of Florence, Florence, ItalyNeuromusculoskeletal Department (Interdisciplinary Internal Medicine), Azienda Ospedaliera Universitaria Careggi, Florence, ItalyIn this study Next-Generation Sequencing (NGS) was used to analyze and compare human microbiota from three different compartments, i.e., saliva, feces, and cancer tissue (CT), of a selected cohort of 10 Italian patients with colorectal cancer (CRC) vs. 10 healthy controls (saliva and feces). Furthermore, the Fusobacterium nucleatum abundance in the same body site was investigated through real-time quantitative polymerase chain reaction (qPCR) to assess the association with CRC. Differences in bacterial composition, F. nucleatum abundance in healthy controls vs. CRC patients, and the association of F. nucleatum with clinical parameters were observed. Taxonomic analysis based on 16S rRNA gene, revealed the presence of three main bacterial phyla, which includes about 80% of reads: Firmicutes (39.18%), Bacteroidetes (30.36%), and Proteobacteria (10.65%). The results highlighted the presence of different bacterial compositions; in particular, the fecal samples of CRC patients seemed to be enriched with Bacteroidetes, whereas in the fecal samples of healthy controls Firmicutes were one of the major phyla detected though these differences were not statistically significant. The CT samples showed the highest alpha diversity values. These results emphasize a different taxonomic composition of feces from CRC compared to healthy controls. Despite the low number of samples included in the study, these results suggest the importance of microbiota in the CRC progression and could pave the way to the development of therapeutic interventions and novel microbial-related diagnostic tools in CRC patients.http://journal.frontiersin.org/article/10.3389/fmicb.2017.02699/fullcolorectal cancergut microbiotaoral microbiotaFusobacterium nucleatumquantitative polymerase chain reactiontaxonomic analysis |
| spellingShingle | Edda Russo Giovanni Bacci Carolina Chiellini Camilla Fagorzi Elena Niccolai Antonio Taddei Federica Ricci Maria N. Ringressi Rossella Borrelli Filippo Melli Manouela Miloeva Paolo Bechi Alessio Mengoni Renato Fani Amedeo Amedei Amedeo Amedei Preliminary Comparison of Oral and Intestinal Human Microbiota in Patients with Colorectal Cancer: A Pilot Study Frontiers in Microbiology colorectal cancer gut microbiota oral microbiota Fusobacterium nucleatum quantitative polymerase chain reaction taxonomic analysis |
| title | Preliminary Comparison of Oral and Intestinal Human Microbiota in Patients with Colorectal Cancer: A Pilot Study |
| title_full | Preliminary Comparison of Oral and Intestinal Human Microbiota in Patients with Colorectal Cancer: A Pilot Study |
| title_fullStr | Preliminary Comparison of Oral and Intestinal Human Microbiota in Patients with Colorectal Cancer: A Pilot Study |
| title_full_unstemmed | Preliminary Comparison of Oral and Intestinal Human Microbiota in Patients with Colorectal Cancer: A Pilot Study |
| title_short | Preliminary Comparison of Oral and Intestinal Human Microbiota in Patients with Colorectal Cancer: A Pilot Study |
| title_sort | preliminary comparison of oral and intestinal human microbiota in patients with colorectal cancer a pilot study |
| topic | colorectal cancer gut microbiota oral microbiota Fusobacterium nucleatum quantitative polymerase chain reaction taxonomic analysis |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02699/full |
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