Ruptura de Tendones Extensores de Dedos por Cubito Plus Idiopático Bilateral
Background: Hyaline cartilage has only a very restricted capability of regeneration in the adult. The incidence of chondral lesions at the knee is high, especially those of Grade II/III (Outerbridge). Therapies combining cells and biological scaffolds are promising biological approaches for the tre...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Asociación Argentina de Ortopedia y Traumatología
2012-11-01
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| Series: | Revista de la Asociación Argentina de Ortopedia y Traumatología |
| Subjects: | |
| Online Access: | http://ojs.aaot.org.ar/ojsr/index.php/AAOTMAG/article/view/39 |
| _version_ | 1827614665890332672 |
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| author | Gustavo Alberto Breglia Néstor Matías Fracalossi |
| author_facet | Gustavo Alberto Breglia Néstor Matías Fracalossi |
| author_sort | Gustavo Alberto Breglia |
| collection | DOAJ |
| description | Background:
Hyaline cartilage has only a very restricted capability of regeneration in the adult. The incidence of chondral lesions at the knee is high, especially those of Grade II/III (Outerbridge). Therapies combining cells and biological scaffolds are promising biological approaches for the treatment of cartilage defects. The aim of this study is to analyze the characteristics of in vitro culture of human chondrocytes on decellularized amniochorionic membrane (ACM).
Methods:
Between December 2010 and December 2011, 16 samples of cartilage from a living donor were processed, but only 7 of them were analyzed. Chondrocytes were grown and amplified on plastic and on ACM. The following analyses were carried out with those cells: interactions between cells and ACM; ACM capacity as a matrix for cells; and behavior of cells cultured on ACM.
Results:
In vitro chondrocytes exhibited phenotypic changes in the presence of ACM. The cells were able to adhere and remain on the spongy region of the membrane. Electron microscopy of cultured ACM showed cells, well preserved organelles, endoplasmic reticulum and desmosomes junctions.
Conclusions:
The feasibility of culturing chondrocytes on ACM was shown in this work. The cells were able to adhere, remain and differentiate on this membrane during the study period. |
| first_indexed | 2024-03-09T08:59:08Z |
| format | Article |
| id | doaj.art-5b32fdf3614e4c899f5cfa55b3a6f435 |
| institution | Directory Open Access Journal |
| issn | 1515-1786 1852-7434 |
| language | English |
| last_indexed | 2024-03-09T08:59:08Z |
| publishDate | 2012-11-01 |
| publisher | Asociación Argentina de Ortopedia y Traumatología |
| record_format | Article |
| series | Revista de la Asociación Argentina de Ortopedia y Traumatología |
| spelling | doaj.art-5b32fdf3614e4c899f5cfa55b3a6f4352023-12-02T12:06:50ZengAsociación Argentina de Ortopedia y TraumatologíaRevista de la Asociación Argentina de Ortopedia y Traumatología1515-17861852-74342012-11-0177319719910.15417/3911Ruptura de Tendones Extensores de Dedos por Cubito Plus Idiopático BilateralGustavo Alberto BregliaNéstor Matías FracalossiBackground: Hyaline cartilage has only a very restricted capability of regeneration in the adult. The incidence of chondral lesions at the knee is high, especially those of Grade II/III (Outerbridge). Therapies combining cells and biological scaffolds are promising biological approaches for the treatment of cartilage defects. The aim of this study is to analyze the characteristics of in vitro culture of human chondrocytes on decellularized amniochorionic membrane (ACM). Methods: Between December 2010 and December 2011, 16 samples of cartilage from a living donor were processed, but only 7 of them were analyzed. Chondrocytes were grown and amplified on plastic and on ACM. The following analyses were carried out with those cells: interactions between cells and ACM; ACM capacity as a matrix for cells; and behavior of cells cultured on ACM. Results: In vitro chondrocytes exhibited phenotypic changes in the presence of ACM. The cells were able to adhere and remain on the spongy region of the membrane. Electron microscopy of cultured ACM showed cells, well preserved organelles, endoplasmic reticulum and desmosomes junctions. Conclusions: The feasibility of culturing chondrocytes on ACM was shown in this work. The cells were able to adhere, remain and differentiate on this membrane during the study period.http://ojs.aaot.org.ar/ojsr/index.php/AAOTMAG/article/view/39ruptura tendón extensorcubito plus |
| spellingShingle | Gustavo Alberto Breglia Néstor Matías Fracalossi Ruptura de Tendones Extensores de Dedos por Cubito Plus Idiopático Bilateral Revista de la Asociación Argentina de Ortopedia y Traumatología ruptura tendón extensor cubito plus |
| title | Ruptura de Tendones Extensores de Dedos por Cubito Plus Idiopático Bilateral |
| title_full | Ruptura de Tendones Extensores de Dedos por Cubito Plus Idiopático Bilateral |
| title_fullStr | Ruptura de Tendones Extensores de Dedos por Cubito Plus Idiopático Bilateral |
| title_full_unstemmed | Ruptura de Tendones Extensores de Dedos por Cubito Plus Idiopático Bilateral |
| title_short | Ruptura de Tendones Extensores de Dedos por Cubito Plus Idiopático Bilateral |
| title_sort | ruptura de tendones extensores de dedos por cubito plus idiopatico bilateral |
| topic | ruptura tendón extensor cubito plus |
| url | http://ojs.aaot.org.ar/ojsr/index.php/AAOTMAG/article/view/39 |
| work_keys_str_mv | AT gustavoalbertobreglia rupturadetendonesextensoresdededosporcubitoplusidiopaticobilateral AT nestormatiasfracalossi rupturadetendonesextensoresdededosporcubitoplusidiopaticobilateral |