Transcriptome Analysis of Immune Response against <i>Streptococcus agalactiae</i> Infection in the Nile Tilapia GIFT Strain
<i>Streptococcus agalactiae</i> (group B streptococcus, GBS), a broad-spectrum pathogen, causes great economic losses in fish aquaculture, especially the industry of tilapia. Until now, the knowledge of the immune response mechanism against <i>S. agalactiae</i> infection in t...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Fishes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2410-3888/7/5/246 |
| _version_ | 1797473360746119168 |
|---|---|
| author | Tao Zhou Zhihua Fang Daniel F. C. Duarte Stefan A. Fernandes Ying Lu Jing Guo Lang Gui Liangbiao Chen |
| author_facet | Tao Zhou Zhihua Fang Daniel F. C. Duarte Stefan A. Fernandes Ying Lu Jing Guo Lang Gui Liangbiao Chen |
| author_sort | Tao Zhou |
| collection | DOAJ |
| description | <i>Streptococcus agalactiae</i> (group B streptococcus, GBS), a broad-spectrum pathogen, causes great economic losses in fish aquaculture, especially the industry of tilapia. Until now, the knowledge of the immune response mechanism against <i>S. agalactiae</i> infection in tilapia has been limited. In the present study, the gill transcriptome of the tilapia from the GBS and the phosphate buffered saline (PBS) groups were sequenced. The transcriptomic analysis results presented the differentially expressed genes (DEGs) at different time points (DEGs number, 6 h: 2122, 9 h: 1851, 15 h: 1791, and 18 h: 2395) after GBS injection, and significantly enriched immune-related gene ontology (GO) terms such as the innate immune response. The significantly enriched immune pathways included the Toll-like receptor signaling pathway, the nucleotide oligomerization domain (NOD)-like receptor signaling pathway, the cytosolic-DNA sensing pathway, and the intestinal immune network for Immunoglobulin A (IgA) production. Most of the DEGs in Toll-like receptor signaling, NOD-like receptor signaling, and cytosolic-DNA sensing pathways presented upregulations at 18 h, which indicated that the innate immune pathways were activated. Two immune-related pathways (phagosome and cell adhesion molecules) were significantly enriched at all time points, suggesting that these two pathways might also play important roles in the immune response against the GBS infection. The results of HE staining showed that the gills of tilapia were damaged seriously at 9 h post-infection, which might be due to the possibility of pyroptosis resulting from the changes of DEGs in the NOD-like receptor signaling pathway. This study provided new insight into the mechanisms of gill damage in fish infected with <i>S. agalactiae</i>. |
| first_indexed | 2024-03-09T20:13:31Z |
| format | Article |
| id | doaj.art-5b350cf661fd4acbb77c0ee431f576ef |
| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-09T20:13:31Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj.art-5b350cf661fd4acbb77c0ee431f576ef2023-11-24T00:06:10ZengMDPI AGFishes2410-38882022-09-017524610.3390/fishes7050246Transcriptome Analysis of Immune Response against <i>Streptococcus agalactiae</i> Infection in the Nile Tilapia GIFT StrainTao Zhou0Zhihua Fang1Daniel F. C. Duarte2Stefan A. Fernandes3Ying Lu4Jing Guo5Lang Gui6Liangbiao Chen7Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, ChinaFaculty of Sciences and Technology, University of Algarve, 8005-139 Faro, PortugalFaculty of Sciences and Technology, University of Algarve, 8005-139 Faro, PortugalKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China<i>Streptococcus agalactiae</i> (group B streptococcus, GBS), a broad-spectrum pathogen, causes great economic losses in fish aquaculture, especially the industry of tilapia. Until now, the knowledge of the immune response mechanism against <i>S. agalactiae</i> infection in tilapia has been limited. In the present study, the gill transcriptome of the tilapia from the GBS and the phosphate buffered saline (PBS) groups were sequenced. The transcriptomic analysis results presented the differentially expressed genes (DEGs) at different time points (DEGs number, 6 h: 2122, 9 h: 1851, 15 h: 1791, and 18 h: 2395) after GBS injection, and significantly enriched immune-related gene ontology (GO) terms such as the innate immune response. The significantly enriched immune pathways included the Toll-like receptor signaling pathway, the nucleotide oligomerization domain (NOD)-like receptor signaling pathway, the cytosolic-DNA sensing pathway, and the intestinal immune network for Immunoglobulin A (IgA) production. Most of the DEGs in Toll-like receptor signaling, NOD-like receptor signaling, and cytosolic-DNA sensing pathways presented upregulations at 18 h, which indicated that the innate immune pathways were activated. Two immune-related pathways (phagosome and cell adhesion molecules) were significantly enriched at all time points, suggesting that these two pathways might also play important roles in the immune response against the GBS infection. The results of HE staining showed that the gills of tilapia were damaged seriously at 9 h post-infection, which might be due to the possibility of pyroptosis resulting from the changes of DEGs in the NOD-like receptor signaling pathway. This study provided new insight into the mechanisms of gill damage in fish infected with <i>S. agalactiae</i>.https://www.mdpi.com/2410-3888/7/5/246transcriptomic<i>Streptococcus agalactiae</i> (GBS)tilapiainnate immune pathwaygill |
| spellingShingle | Tao Zhou Zhihua Fang Daniel F. C. Duarte Stefan A. Fernandes Ying Lu Jing Guo Lang Gui Liangbiao Chen Transcriptome Analysis of Immune Response against <i>Streptococcus agalactiae</i> Infection in the Nile Tilapia GIFT Strain Fishes transcriptomic <i>Streptococcus agalactiae</i> (GBS) tilapia innate immune pathway gill |
| title | Transcriptome Analysis of Immune Response against <i>Streptococcus agalactiae</i> Infection in the Nile Tilapia GIFT Strain |
| title_full | Transcriptome Analysis of Immune Response against <i>Streptococcus agalactiae</i> Infection in the Nile Tilapia GIFT Strain |
| title_fullStr | Transcriptome Analysis of Immune Response against <i>Streptococcus agalactiae</i> Infection in the Nile Tilapia GIFT Strain |
| title_full_unstemmed | Transcriptome Analysis of Immune Response against <i>Streptococcus agalactiae</i> Infection in the Nile Tilapia GIFT Strain |
| title_short | Transcriptome Analysis of Immune Response against <i>Streptococcus agalactiae</i> Infection in the Nile Tilapia GIFT Strain |
| title_sort | transcriptome analysis of immune response against i streptococcus agalactiae i infection in the nile tilapia gift strain |
| topic | transcriptomic <i>Streptococcus agalactiae</i> (GBS) tilapia innate immune pathway gill |
| url | https://www.mdpi.com/2410-3888/7/5/246 |
| work_keys_str_mv | AT taozhou transcriptomeanalysisofimmuneresponseagainstistreptococcusagalactiaeiinfectionintheniletilapiagiftstrain AT zhihuafang transcriptomeanalysisofimmuneresponseagainstistreptococcusagalactiaeiinfectionintheniletilapiagiftstrain AT danielfcduarte transcriptomeanalysisofimmuneresponseagainstistreptococcusagalactiaeiinfectionintheniletilapiagiftstrain AT stefanafernandes transcriptomeanalysisofimmuneresponseagainstistreptococcusagalactiaeiinfectionintheniletilapiagiftstrain AT yinglu transcriptomeanalysisofimmuneresponseagainstistreptococcusagalactiaeiinfectionintheniletilapiagiftstrain AT jingguo transcriptomeanalysisofimmuneresponseagainstistreptococcusagalactiaeiinfectionintheniletilapiagiftstrain AT langgui transcriptomeanalysisofimmuneresponseagainstistreptococcusagalactiaeiinfectionintheniletilapiagiftstrain AT liangbiaochen transcriptomeanalysisofimmuneresponseagainstistreptococcusagalactiaeiinfectionintheniletilapiagiftstrain |