Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury
Spinal cord injury (SCI) is a disabling condition resulting in deficits of sensory and motor functions, and has no effective treatment. Considering that protocols with stem cell transplantation and treadmill training have shown promising results, the present study evaluated the effectiveness of stem...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2016-08-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/pdf/bjmbr/v49n9/1414-431X-bjmbr-1414-431X20165319.pdf |
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| author | F.C. Nicola L.P. Rodrigues T. Crestani K. Quintiliano E.F. Sanches S. Willborn D. Aristimunha L. Boisserand P. Pranke C.A. Netto |
| author_facet | F.C. Nicola L.P. Rodrigues T. Crestani K. Quintiliano E.F. Sanches S. Willborn D. Aristimunha L. Boisserand P. Pranke C.A. Netto |
| author_sort | F.C. Nicola |
| collection | DOAJ |
| description | Spinal cord injury (SCI) is a disabling condition resulting in deficits of sensory and motor functions, and has no effective treatment. Considering that protocols with stem cell transplantation and treadmill training have shown promising results, the present study evaluated the effectiveness of stem cells from human exfoliated deciduous teeth (SHEDs) transplantation combined with treadmill training in rats with experimental spinal cord injury. Fifty-four Wistar rats were spinalized using NYU impactor. The rats were randomly distributed into 5 groups: Sham (laminectomy with no SCI, n=10); SCI (laminectomy followed by SCI, n=12); SHEDs (SCI treated with SHEDs, n=11); TT (SCI treated with treadmill training, n=11); SHEDs+TT (SCI treated with SHEDs and treadmill training; n=10). Treatment with SHEDs alone or in combination with treadmill training promoted functional recovery, reaching scores of 15 and 14, respectively, in the BBB scale, being different from the SCI group, which reached 11. SHEDs treatment was able to reduce the cystic cavity area and glial scar, increase neurofilament. Treadmill training alone had no functional effectiveness or tissue effects. In a second experiment, the SHEDs transplantation reduced the TNF-α levels in the cord tissue measured 6 h after the injury. Contrary to our hypothesis, treadmill training either alone or in combination, caused no functional improvement. However, SHEDs showed to be neuroprotective, by the reduction of TNF-α levels, the cystic cavity and the glial scar associated with the improvement of motor function after SCI. These results provide evidence that grafted SHEDs might be an effective therapy to spinal cord lesions, with possible anti-inflammatory action. |
| first_indexed | 2024-12-18T02:35:28Z |
| format | Article |
| id | doaj.art-5b3671fd05174e618020465b674c2eb6 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-12-18T02:35:28Z |
| publishDate | 2016-08-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-5b3671fd05174e618020465b674c2eb62022-12-21T21:23:47ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2016-08-0149910.1590/1414-431x20165319Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injuryF.C. NicolaL.P. RodriguesT. CrestaniK. QuintilianoE.F. SanchesS. WillbornD. AristimunhaL. BoisserandP. PrankeC.A. NettoSpinal cord injury (SCI) is a disabling condition resulting in deficits of sensory and motor functions, and has no effective treatment. Considering that protocols with stem cell transplantation and treadmill training have shown promising results, the present study evaluated the effectiveness of stem cells from human exfoliated deciduous teeth (SHEDs) transplantation combined with treadmill training in rats with experimental spinal cord injury. Fifty-four Wistar rats were spinalized using NYU impactor. The rats were randomly distributed into 5 groups: Sham (laminectomy with no SCI, n=10); SCI (laminectomy followed by SCI, n=12); SHEDs (SCI treated with SHEDs, n=11); TT (SCI treated with treadmill training, n=11); SHEDs+TT (SCI treated with SHEDs and treadmill training; n=10). Treatment with SHEDs alone or in combination with treadmill training promoted functional recovery, reaching scores of 15 and 14, respectively, in the BBB scale, being different from the SCI group, which reached 11. SHEDs treatment was able to reduce the cystic cavity area and glial scar, increase neurofilament. Treadmill training alone had no functional effectiveness or tissue effects. In a second experiment, the SHEDs transplantation reduced the TNF-α levels in the cord tissue measured 6 h after the injury. Contrary to our hypothesis, treadmill training either alone or in combination, caused no functional improvement. However, SHEDs showed to be neuroprotective, by the reduction of TNF-α levels, the cystic cavity and the glial scar associated with the improvement of motor function after SCI. These results provide evidence that grafted SHEDs might be an effective therapy to spinal cord lesions, with possible anti-inflammatory action.http://www.scielo.br/pdf/bjmbr/v49n9/1414-431X-bjmbr-1414-431X20165319.pdfSpinal cord injuryStem cellsTreadmill trainingFunctional recoveryMASCIS impactor |
| spellingShingle | F.C. Nicola L.P. Rodrigues T. Crestani K. Quintiliano E.F. Sanches S. Willborn D. Aristimunha L. Boisserand P. Pranke C.A. Netto Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury Brazilian Journal of Medical and Biological Research Spinal cord injury Stem cells Treadmill training Functional recovery MASCIS impactor |
| title | Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury |
| title_full | Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury |
| title_fullStr | Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury |
| title_full_unstemmed | Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury |
| title_short | Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury |
| title_sort | human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury |
| topic | Spinal cord injury Stem cells Treadmill training Functional recovery MASCIS impactor |
| url | http://www.scielo.br/pdf/bjmbr/v49n9/1414-431X-bjmbr-1414-431X20165319.pdf |
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