Alterations in B Cell and Follicular T-Helper Cell Subsets in Patients with Acute COVID-19 and COVID-19 Convalescents
Background. Humoral immunity requires interaction between B cell and T follicular helper cells (Tfh) to produce effective immune response, but the data regarding a role of B cells and Tfh in SARS-CoV-2 defense are still sparse. Methods. Blood samples from patients with acute COVID-19 (<i>n<...
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2021-12-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/44/1/14 |
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| author | Igor V. Kudryavtsev Natalia A. Arsentieva Oleg K. Batsunov Zoia R. Korobova Irina V. Khamitova Dmitrii V. Isakov Raisa N. Kuznetsova Artem A. Rubinstein Oksana V. Stanevich Aleksandra A. Lebedeva Evgeny A. Vorobyov Snejana V. Vorobyova Alexander N. Kulikov Maria A. Sharapova Dmitrii E. Pevtcov Areg A. Totolian |
| author_facet | Igor V. Kudryavtsev Natalia A. Arsentieva Oleg K. Batsunov Zoia R. Korobova Irina V. Khamitova Dmitrii V. Isakov Raisa N. Kuznetsova Artem A. Rubinstein Oksana V. Stanevich Aleksandra A. Lebedeva Evgeny A. Vorobyov Snejana V. Vorobyova Alexander N. Kulikov Maria A. Sharapova Dmitrii E. Pevtcov Areg A. Totolian |
| author_sort | Igor V. Kudryavtsev |
| collection | DOAJ |
| description | Background. Humoral immunity requires interaction between B cell and T follicular helper cells (Tfh) to produce effective immune response, but the data regarding a role of B cells and Tfh in SARS-CoV-2 defense are still sparse. Methods. Blood samples from patients with acute COVID-19 (<i>n</i> = 64), convalescents patients who had specific IgG to SARS-CoV-2 N-protein (<i>n</i> = 55), and healthy donors with no detectable antibodies to any SARS-CoV-2 proteins (HC, <i>n</i> = 44) were analyses by multicolor flow cytometry. Results. Patients with acute COVID-19 showed decreased levels of memory B cells subsets and increased proportion plasma cell precursors compared to HC and COVID-19 convalescent patients, whereas for the latter the elevated numbers of virgin naïve, Bm2′ and “Bm3+Bm4” was found if compared with HC. During acute COVID-19 CXCR3+CCR6− Tfh1-like cells were decreased and the levels of CXCR3−CCR6+ Tfh17-like were increased then in HC and convalescent patients. Finally, COVID-19 convalescent patients had increased levels of Tfh2-, Tfh17- and DP Tfh-like cells while comparing their amount with HC. Conclusions. Our data indicate that COVID-19 can impact the humoral immunity in the long-term. |
| first_indexed | 2024-03-10T01:42:15Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-10T01:42:15Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-5b36db7bb0c149ff81525d488b0ed0c32023-11-23T13:22:30ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452021-12-0144119420510.3390/cimb44010014Alterations in B Cell and Follicular T-Helper Cell Subsets in Patients with Acute COVID-19 and COVID-19 ConvalescentsIgor V. Kudryavtsev0Natalia A. Arsentieva1Oleg K. Batsunov2Zoia R. Korobova3Irina V. Khamitova4Dmitrii V. Isakov5Raisa N. Kuznetsova6Artem A. Rubinstein7Oksana V. Stanevich8Aleksandra A. Lebedeva9Evgeny A. Vorobyov10Snejana V. Vorobyova11Alexander N. Kulikov12Maria A. Sharapova13Dmitrii E. Pevtcov14Areg A. Totolian15Institute of Experimental Medicine, Akademika Pavlova 12, 197376 Saint Petersburg, RussiaLaboratory of Immunology, Saint Petersburg Pasteur Institute, Mira 14, 197101 Saint Petersburg, RussiaMedical Facutly, First Saint Petersburg State I. Pavlov Medical University, L’va Tolstogo St. 6-8, 197022 Saint Petersburg, RussiaLaboratory of Immunology, Saint Petersburg Pasteur Institute, Mira 14, 197101 Saint Petersburg, RussiaLaboratory of Immunology, Saint Petersburg Pasteur Institute, Mira 14, 197101 Saint Petersburg, RussiaInstitute of Experimental Medicine, Akademika Pavlova 12, 197376 Saint Petersburg, RussiaMedical Facutly, First Saint Petersburg State I. Pavlov Medical University, L’va Tolstogo St. 6-8, 197022 Saint Petersburg, RussiaMedical Facutly, First Saint Petersburg State I. Pavlov Medical University, L’va Tolstogo St. 6-8, 197022 Saint Petersburg, RussiaMedical Facutly, First Saint Petersburg State I. Pavlov Medical University, L’va Tolstogo St. 6-8, 197022 Saint Petersburg, RussiaMedical Facutly, First Saint Petersburg State I. Pavlov Medical University, L’va Tolstogo St. 6-8, 197022 Saint Petersburg, RussiaMedical Facutly, First Saint Petersburg State I. Pavlov Medical University, L’va Tolstogo St. 6-8, 197022 Saint Petersburg, RussiaMedical Facutly, First Saint Petersburg State I. Pavlov Medical University, L’va Tolstogo St. 6-8, 197022 Saint Petersburg, RussiaMedical Facutly, First Saint Petersburg State I. Pavlov Medical University, L’va Tolstogo St. 6-8, 197022 Saint Petersburg, RussiaMedical Facutly, First Saint Petersburg State I. Pavlov Medical University, L’va Tolstogo St. 6-8, 197022 Saint Petersburg, RussiaMedical Facutly, First Saint Petersburg State I. Pavlov Medical University, L’va Tolstogo St. 6-8, 197022 Saint Petersburg, RussiaMedical Facutly, First Saint Petersburg State I. Pavlov Medical University, L’va Tolstogo St. 6-8, 197022 Saint Petersburg, RussiaBackground. Humoral immunity requires interaction between B cell and T follicular helper cells (Tfh) to produce effective immune response, but the data regarding a role of B cells and Tfh in SARS-CoV-2 defense are still sparse. Methods. Blood samples from patients with acute COVID-19 (<i>n</i> = 64), convalescents patients who had specific IgG to SARS-CoV-2 N-protein (<i>n</i> = 55), and healthy donors with no detectable antibodies to any SARS-CoV-2 proteins (HC, <i>n</i> = 44) were analyses by multicolor flow cytometry. Results. Patients with acute COVID-19 showed decreased levels of memory B cells subsets and increased proportion plasma cell precursors compared to HC and COVID-19 convalescent patients, whereas for the latter the elevated numbers of virgin naïve, Bm2′ and “Bm3+Bm4” was found if compared with HC. During acute COVID-19 CXCR3+CCR6− Tfh1-like cells were decreased and the levels of CXCR3−CCR6+ Tfh17-like were increased then in HC and convalescent patients. Finally, COVID-19 convalescent patients had increased levels of Tfh2-, Tfh17- and DP Tfh-like cells while comparing their amount with HC. Conclusions. Our data indicate that COVID-19 can impact the humoral immunity in the long-term.https://www.mdpi.com/1467-3045/44/1/14COVID-19SARS-CoV-2B cell subsetsT follicular helper cellsTfh cell subsetsconvalescents |
| spellingShingle | Igor V. Kudryavtsev Natalia A. Arsentieva Oleg K. Batsunov Zoia R. Korobova Irina V. Khamitova Dmitrii V. Isakov Raisa N. Kuznetsova Artem A. Rubinstein Oksana V. Stanevich Aleksandra A. Lebedeva Evgeny A. Vorobyov Snejana V. Vorobyova Alexander N. Kulikov Maria A. Sharapova Dmitrii E. Pevtcov Areg A. Totolian Alterations in B Cell and Follicular T-Helper Cell Subsets in Patients with Acute COVID-19 and COVID-19 Convalescents Current Issues in Molecular Biology COVID-19 SARS-CoV-2 B cell subsets T follicular helper cells Tfh cell subsets convalescents |
| title | Alterations in B Cell and Follicular T-Helper Cell Subsets in Patients with Acute COVID-19 and COVID-19 Convalescents |
| title_full | Alterations in B Cell and Follicular T-Helper Cell Subsets in Patients with Acute COVID-19 and COVID-19 Convalescents |
| title_fullStr | Alterations in B Cell and Follicular T-Helper Cell Subsets in Patients with Acute COVID-19 and COVID-19 Convalescents |
| title_full_unstemmed | Alterations in B Cell and Follicular T-Helper Cell Subsets in Patients with Acute COVID-19 and COVID-19 Convalescents |
| title_short | Alterations in B Cell and Follicular T-Helper Cell Subsets in Patients with Acute COVID-19 and COVID-19 Convalescents |
| title_sort | alterations in b cell and follicular t helper cell subsets in patients with acute covid 19 and covid 19 convalescents |
| topic | COVID-19 SARS-CoV-2 B cell subsets T follicular helper cells Tfh cell subsets convalescents |
| url | https://www.mdpi.com/1467-3045/44/1/14 |
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