A Cartilage Matrix Protein Regulates Collagen Synthesis in Mantle of <i>Magallana gigas</i> (<i>Crassostrea gigas</i>) under Ocean Acidification
The shell biosynthesis of oysters plays a critical role in protection against environmental stress, in which cartilage matrix proteins (CMPs) determine the mineralogical and crystallographic properties of the shell. In the present study, a cartilage matrix protein (designated as <i>Mg</i>...
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MDPI AG
2023-05-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/8/6/290 |
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| author | Ting Zhu Chang Liu Zhaoqun Liu Yuqian Gao Xiaoyu Xin Lingling Wang Linsheng Song |
| author_facet | Ting Zhu Chang Liu Zhaoqun Liu Yuqian Gao Xiaoyu Xin Lingling Wang Linsheng Song |
| author_sort | Ting Zhu |
| collection | DOAJ |
| description | The shell biosynthesis of oysters plays a critical role in protection against environmental stress, in which cartilage matrix proteins (CMPs) determine the mineralogical and crystallographic properties of the shell. In the present study, a cartilage matrix protein (designated as <i>Mg</i>CMP1) was identified from the Pacific oyster <i>Magallana gigas</i> (<i>Crassostrea gigas</i>) with the objective of understanding its possible role in shell formation. The open reading frame (ORF) of <i>Mg</i>CMP1 was 1815 bp, encoding a polypeptide of 605 amino acids with two von Willebrand factor (VWA) domains. The mRNA transcript of <i>Mg</i>CMP1 was expressed constitutively in all examined tissues with a higher level in the mantle, especially highest in the middle fold (MF) of the three folds of the mantle. In addition, the interaction between recombinant protein MgCMP1 (rMgCMP1) and recombinant protein bone morphogenesis protein 7 (rMgBMP7) was identified in vitro. After injection of dsRNA to inhibit the expression of <i>Mg</i>CMP1, the mRNA expression level of <i>Mg</i>collagen I and <i>Mg</i>collagen X in the MF of the mantle significantly decreased. After pre-puncturing and acidification treatment (pH 7.8), the thickness and length of the new formation shells were lower than those in control group (pH 8.1), and the positive hybridization signals of the <i>Mg</i>CMP1 mRNA transcript in the three mantle folds were obviously weakened, especially in the MF, whereas the mRNA expression level of <i>Mg</i>CMP1, <i>Mg</i>collagen I and <i>Mg</i>collagen X in the MF of mantle decreased significantly. These results suggested that <i>Mg</i>CMP1 was involved in regulating the expression of <i>Mg</i>collagen I and <i>Mg</i>collagen X in the MF of the mantle in response to ocean acidification (OA). |
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| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-11T02:28:40Z |
| publishDate | 2023-05-01 |
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| spelling | doaj.art-5b3aea6de39d4c398d1fe829bdd0276b2023-11-18T10:22:33ZengMDPI AGFishes2410-38882023-05-018629010.3390/fishes8060290A Cartilage Matrix Protein Regulates Collagen Synthesis in Mantle of <i>Magallana gigas</i> (<i>Crassostrea gigas</i>) under Ocean AcidificationTing Zhu0Chang Liu1Zhaoqun Liu2Yuqian Gao3Xiaoyu Xin4Lingling Wang5Linsheng Song6Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, ChinaLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, ChinaLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, ChinaLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, ChinaLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, ChinaLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, ChinaLiaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, ChinaThe shell biosynthesis of oysters plays a critical role in protection against environmental stress, in which cartilage matrix proteins (CMPs) determine the mineralogical and crystallographic properties of the shell. In the present study, a cartilage matrix protein (designated as <i>Mg</i>CMP1) was identified from the Pacific oyster <i>Magallana gigas</i> (<i>Crassostrea gigas</i>) with the objective of understanding its possible role in shell formation. The open reading frame (ORF) of <i>Mg</i>CMP1 was 1815 bp, encoding a polypeptide of 605 amino acids with two von Willebrand factor (VWA) domains. The mRNA transcript of <i>Mg</i>CMP1 was expressed constitutively in all examined tissues with a higher level in the mantle, especially highest in the middle fold (MF) of the three folds of the mantle. In addition, the interaction between recombinant protein MgCMP1 (rMgCMP1) and recombinant protein bone morphogenesis protein 7 (rMgBMP7) was identified in vitro. After injection of dsRNA to inhibit the expression of <i>Mg</i>CMP1, the mRNA expression level of <i>Mg</i>collagen I and <i>Mg</i>collagen X in the MF of the mantle significantly decreased. After pre-puncturing and acidification treatment (pH 7.8), the thickness and length of the new formation shells were lower than those in control group (pH 8.1), and the positive hybridization signals of the <i>Mg</i>CMP1 mRNA transcript in the three mantle folds were obviously weakened, especially in the MF, whereas the mRNA expression level of <i>Mg</i>CMP1, <i>Mg</i>collagen I and <i>Mg</i>collagen X in the MF of mantle decreased significantly. These results suggested that <i>Mg</i>CMP1 was involved in regulating the expression of <i>Mg</i>collagen I and <i>Mg</i>collagen X in the MF of the mantle in response to ocean acidification (OA).https://www.mdpi.com/2410-3888/8/6/290ocean acidification<i>Magallana gigas</i> (<i>Crassostrea gigas</i>)cartilage matrix proteinmiddle fold of the mantlecollagen |
| spellingShingle | Ting Zhu Chang Liu Zhaoqun Liu Yuqian Gao Xiaoyu Xin Lingling Wang Linsheng Song A Cartilage Matrix Protein Regulates Collagen Synthesis in Mantle of <i>Magallana gigas</i> (<i>Crassostrea gigas</i>) under Ocean Acidification Fishes ocean acidification <i>Magallana gigas</i> (<i>Crassostrea gigas</i>) cartilage matrix protein middle fold of the mantle collagen |
| title | A Cartilage Matrix Protein Regulates Collagen Synthesis in Mantle of <i>Magallana gigas</i> (<i>Crassostrea gigas</i>) under Ocean Acidification |
| title_full | A Cartilage Matrix Protein Regulates Collagen Synthesis in Mantle of <i>Magallana gigas</i> (<i>Crassostrea gigas</i>) under Ocean Acidification |
| title_fullStr | A Cartilage Matrix Protein Regulates Collagen Synthesis in Mantle of <i>Magallana gigas</i> (<i>Crassostrea gigas</i>) under Ocean Acidification |
| title_full_unstemmed | A Cartilage Matrix Protein Regulates Collagen Synthesis in Mantle of <i>Magallana gigas</i> (<i>Crassostrea gigas</i>) under Ocean Acidification |
| title_short | A Cartilage Matrix Protein Regulates Collagen Synthesis in Mantle of <i>Magallana gigas</i> (<i>Crassostrea gigas</i>) under Ocean Acidification |
| title_sort | cartilage matrix protein regulates collagen synthesis in mantle of i magallana gigas i i crassostrea gigas i under ocean acidification |
| topic | ocean acidification <i>Magallana gigas</i> (<i>Crassostrea gigas</i>) cartilage matrix protein middle fold of the mantle collagen |
| url | https://www.mdpi.com/2410-3888/8/6/290 |
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