Inorganic phosphate modifies stationary phase fitness and metabolic pathways in Lactiplantibacillus paraplantarum CRL 1905
Inorganic phosphate (Pi) concentration modulates polyphosphate (polyP) levels in diverse bacteria, affecting their physiology and survival. Lactiplantibacillus paraplantarum CRL 1905 is a lactic acid bacterium isolated from quinoa sourdough with biotechnological potential as starter, for initiating...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-02-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1343541/full |
| _version_ | 1797294131588890624 |
|---|---|
| author | Mario Araoz Mariana Grillo-Puertas Alejandra de Moreno de LeBlanc Elvira María Hebert Josefina María Villegas Viviana Andrea Rapisarda |
| author_facet | Mario Araoz Mariana Grillo-Puertas Alejandra de Moreno de LeBlanc Elvira María Hebert Josefina María Villegas Viviana Andrea Rapisarda |
| author_sort | Mario Araoz |
| collection | DOAJ |
| description | Inorganic phosphate (Pi) concentration modulates polyphosphate (polyP) levels in diverse bacteria, affecting their physiology and survival. Lactiplantibacillus paraplantarum CRL 1905 is a lactic acid bacterium isolated from quinoa sourdough with biotechnological potential as starter, for initiating fermentation processes in food, and as antimicrobial-producing organism. The aim of this work was to evaluate the influence of the environmental Pi concentration on different physiological and molecular aspects of the CRL 1905 strain. Cells grown in a chemically defined medium containing high Pi (CDM + P) maintained elevated polyP levels up to late stationary phase and showed an enhanced bacterial survival and tolerance to oxidative stress. In Pi sufficiency condition (CDM-P), cells were ~ 25% longer than those grown in CDM + P, presented membrane vesicles and a ~ 3-fold higher capacity to form biofilm. Proteomic analysis indicated that proteins involved in the “carbohydrate transport and metabolism” and “energy production and conversion” categories were up-regulated in high Pi stationary phase cells, implying an active metabolism in this condition. On the other hand, stress-related chaperones and enzymes involved in cell surface modification were up-regulated in the CDM-P medium. Our results provide new insights to understand the CRL 1905 adaptations in response to differential Pi conditions. The adjustment of environmental Pi concentration constitutes a simple strategy to improve the cellular fitness of L. paraplantarum CRL 1905, which would benefit its potential as a microbial cell factory. |
| first_indexed | 2024-03-07T21:25:48Z |
| format | Article |
| id | doaj.art-5b3e469964584cfcba272c447ccba565 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-07T21:25:48Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5b3e469964584cfcba272c447ccba5652024-02-27T04:52:36ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-02-011510.3389/fmicb.2024.13435411343541Inorganic phosphate modifies stationary phase fitness and metabolic pathways in Lactiplantibacillus paraplantarum CRL 1905Mario Araoz0Mariana Grillo-Puertas1Alejandra de Moreno de LeBlanc2Elvira María Hebert3Josefina María Villegas4Viviana Andrea Rapisarda5Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Química Biológica, “Dr. Bernabé Bloj”, Facultad de Bioquímica, Química y Farmacia, UNT, San Miguel de Tucumán, ArgentinaInstituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Química Biológica, “Dr. Bernabé Bloj”, Facultad de Bioquímica, Química y Farmacia, UNT, San Miguel de Tucumán, ArgentinaCentro de Referencia para Lactobacilos (CERELA-CONICET), San Miguel de Tucumán, ArgentinaCentro de Referencia para Lactobacilos (CERELA-CONICET), San Miguel de Tucumán, ArgentinaInstituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Química Biológica, “Dr. Bernabé Bloj”, Facultad de Bioquímica, Química y Farmacia, UNT, San Miguel de Tucumán, ArgentinaInstituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Química Biológica, “Dr. Bernabé Bloj”, Facultad de Bioquímica, Química y Farmacia, UNT, San Miguel de Tucumán, ArgentinaInorganic phosphate (Pi) concentration modulates polyphosphate (polyP) levels in diverse bacteria, affecting their physiology and survival. Lactiplantibacillus paraplantarum CRL 1905 is a lactic acid bacterium isolated from quinoa sourdough with biotechnological potential as starter, for initiating fermentation processes in food, and as antimicrobial-producing organism. The aim of this work was to evaluate the influence of the environmental Pi concentration on different physiological and molecular aspects of the CRL 1905 strain. Cells grown in a chemically defined medium containing high Pi (CDM + P) maintained elevated polyP levels up to late stationary phase and showed an enhanced bacterial survival and tolerance to oxidative stress. In Pi sufficiency condition (CDM-P), cells were ~ 25% longer than those grown in CDM + P, presented membrane vesicles and a ~ 3-fold higher capacity to form biofilm. Proteomic analysis indicated that proteins involved in the “carbohydrate transport and metabolism” and “energy production and conversion” categories were up-regulated in high Pi stationary phase cells, implying an active metabolism in this condition. On the other hand, stress-related chaperones and enzymes involved in cell surface modification were up-regulated in the CDM-P medium. Our results provide new insights to understand the CRL 1905 adaptations in response to differential Pi conditions. The adjustment of environmental Pi concentration constitutes a simple strategy to improve the cellular fitness of L. paraplantarum CRL 1905, which would benefit its potential as a microbial cell factory.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1343541/fulllactic acid bacteriastationary phasepolyphosphateproteomicsbiofilm |
| spellingShingle | Mario Araoz Mariana Grillo-Puertas Alejandra de Moreno de LeBlanc Elvira María Hebert Josefina María Villegas Viviana Andrea Rapisarda Inorganic phosphate modifies stationary phase fitness and metabolic pathways in Lactiplantibacillus paraplantarum CRL 1905 Frontiers in Microbiology lactic acid bacteria stationary phase polyphosphate proteomics biofilm |
| title | Inorganic phosphate modifies stationary phase fitness and metabolic pathways in Lactiplantibacillus paraplantarum CRL 1905 |
| title_full | Inorganic phosphate modifies stationary phase fitness and metabolic pathways in Lactiplantibacillus paraplantarum CRL 1905 |
| title_fullStr | Inorganic phosphate modifies stationary phase fitness and metabolic pathways in Lactiplantibacillus paraplantarum CRL 1905 |
| title_full_unstemmed | Inorganic phosphate modifies stationary phase fitness and metabolic pathways in Lactiplantibacillus paraplantarum CRL 1905 |
| title_short | Inorganic phosphate modifies stationary phase fitness and metabolic pathways in Lactiplantibacillus paraplantarum CRL 1905 |
| title_sort | inorganic phosphate modifies stationary phase fitness and metabolic pathways in lactiplantibacillus paraplantarum crl 1905 |
| topic | lactic acid bacteria stationary phase polyphosphate proteomics biofilm |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1343541/full |
| work_keys_str_mv | AT marioaraoz inorganicphosphatemodifiesstationaryphasefitnessandmetabolicpathwaysinlactiplantibacillusparaplantarumcrl1905 AT marianagrillopuertas inorganicphosphatemodifiesstationaryphasefitnessandmetabolicpathwaysinlactiplantibacillusparaplantarumcrl1905 AT alejandrademorenodeleblanc inorganicphosphatemodifiesstationaryphasefitnessandmetabolicpathwaysinlactiplantibacillusparaplantarumcrl1905 AT elviramariahebert inorganicphosphatemodifiesstationaryphasefitnessandmetabolicpathwaysinlactiplantibacillusparaplantarumcrl1905 AT josefinamariavillegas inorganicphosphatemodifiesstationaryphasefitnessandmetabolicpathwaysinlactiplantibacillusparaplantarumcrl1905 AT vivianaandrearapisarda inorganicphosphatemodifiesstationaryphasefitnessandmetabolicpathwaysinlactiplantibacillusparaplantarumcrl1905 |