Essential (Mg, Fe, Zn and Cu) and Non-Essential (Cd and Pb) Elements in Predatory Insects (<i>Vespa crabro</i> and <i>Vespa velutina</i>): A Molecular Perspective
The recent introduction of the Asian yellow-legged hornet, <i>Vespa velutina,</i> into Europe has raised concern regarding the threat to honeybees and the competition with the European hornet, <i>Vespa crabro</i>. The aim of this study was to investigated essential (Mg, Fe, Z...
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| Format: | Article |
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MDPI AG
2020-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/1/228 |
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| author | Giulia Andreani Enea Ferlizza Riccardo Cabbri Micaela Fabbri Elisa Bellei Gloria Isani |
| author_facet | Giulia Andreani Enea Ferlizza Riccardo Cabbri Micaela Fabbri Elisa Bellei Gloria Isani |
| author_sort | Giulia Andreani |
| collection | DOAJ |
| description | The recent introduction of the Asian yellow-legged hornet, <i>Vespa velutina,</i> into Europe has raised concern regarding the threat to honeybees and the competition with the European hornet, <i>Vespa crabro</i>. The aim of this study was to investigated essential (Mg, Fe, Zn, Cu) and non-essential (Cd and Pb) elements in these two species. Element concentrations were determined in the whole body and separately in the head, thorax and abdomen using atomic absorption spectrometry (AAS). The changes in essential element concentration and speciation during metamorphosis were also studied using size exclusion chromatography followed by AAS and proteomic analysis. In both species, the essential elements were more concentrated in the abdomen due to the presence of fat bodies. Magnesium, Fe and Zn concentrations were significantly higher in <i>V</i>. <i>crabro</i> than in <i>V. velutina</i> and could have been related to the higher aerobic energy demand of the former species required to sustain foraging flight. Low concentrations of Cd and Pb were indicative of low environmental exposure. The concentration and speciation of essential elements, particularly Fe, varied among the developmental stages, indicating a modification of ligand preferences during metamorphosis. Overall, the results in the present study provide a better understanding of the hornet metal metabolism and a foundation for additional studies. |
| first_indexed | 2024-03-10T13:42:35Z |
| format | Article |
| id | doaj.art-5b3f508ff88947e79ccd6810c0dcb146 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T13:42:35Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5b3f508ff88947e79ccd6810c0dcb1462023-11-21T02:51:58ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-12-0122122810.3390/ijms22010228Essential (Mg, Fe, Zn and Cu) and Non-Essential (Cd and Pb) Elements in Predatory Insects (<i>Vespa crabro</i> and <i>Vespa velutina</i>): A Molecular PerspectiveGiulia Andreani0Enea Ferlizza1Riccardo Cabbri2Micaela Fabbri3Elisa Bellei4Gloria Isani5Department of Veterinary Medical Sciences, Alma Mater Studiorum—University of Bologna, via Tolara di sopra 50, Ozzano dell’Emilia, 40064 Bologna, ItalyDepartment of Experimental Diagnostic and Specialty Medicine, Alma Mater Studiorum-University of Bologna, via Belmeloro 8, 40126 Bologna, ItalyDepartment of Veterinary Medical Sciences, Alma Mater Studiorum—University of Bologna, via Tolara di sopra 50, Ozzano dell’Emilia, 40064 Bologna, ItalyDepartment of Veterinary Medical Sciences, Alma Mater Studiorum—University of Bologna, via Tolara di sopra 50, Ozzano dell’Emilia, 40064 Bologna, ItalyDepartment of Surgery, Medicine, Dentistry and Morphological Sciences with Transplant Surgery, Oncology and Regenerative Medicine Relevance, Proteomic Lab, University of Modena and Reggio Emilia, via del pozzo 71, 41124 Modena, ItalyDepartment of Veterinary Medical Sciences, Alma Mater Studiorum—University of Bologna, via Tolara di sopra 50, Ozzano dell’Emilia, 40064 Bologna, ItalyThe recent introduction of the Asian yellow-legged hornet, <i>Vespa velutina,</i> into Europe has raised concern regarding the threat to honeybees and the competition with the European hornet, <i>Vespa crabro</i>. The aim of this study was to investigated essential (Mg, Fe, Zn, Cu) and non-essential (Cd and Pb) elements in these two species. Element concentrations were determined in the whole body and separately in the head, thorax and abdomen using atomic absorption spectrometry (AAS). The changes in essential element concentration and speciation during metamorphosis were also studied using size exclusion chromatography followed by AAS and proteomic analysis. In both species, the essential elements were more concentrated in the abdomen due to the presence of fat bodies. Magnesium, Fe and Zn concentrations were significantly higher in <i>V</i>. <i>crabro</i> than in <i>V. velutina</i> and could have been related to the higher aerobic energy demand of the former species required to sustain foraging flight. Low concentrations of Cd and Pb were indicative of low environmental exposure. The concentration and speciation of essential elements, particularly Fe, varied among the developmental stages, indicating a modification of ligand preferences during metamorphosis. Overall, the results in the present study provide a better understanding of the hornet metal metabolism and a foundation for additional studies.https://www.mdpi.com/1422-0067/22/1/228Asian yellow-legged hornetEuropean hornetelectrophoresisproteomicsironcopper |
| spellingShingle | Giulia Andreani Enea Ferlizza Riccardo Cabbri Micaela Fabbri Elisa Bellei Gloria Isani Essential (Mg, Fe, Zn and Cu) and Non-Essential (Cd and Pb) Elements in Predatory Insects (<i>Vespa crabro</i> and <i>Vespa velutina</i>): A Molecular Perspective International Journal of Molecular Sciences Asian yellow-legged hornet European hornet electrophoresis proteomics iron copper |
| title | Essential (Mg, Fe, Zn and Cu) and Non-Essential (Cd and Pb) Elements in Predatory Insects (<i>Vespa crabro</i> and <i>Vespa velutina</i>): A Molecular Perspective |
| title_full | Essential (Mg, Fe, Zn and Cu) and Non-Essential (Cd and Pb) Elements in Predatory Insects (<i>Vespa crabro</i> and <i>Vespa velutina</i>): A Molecular Perspective |
| title_fullStr | Essential (Mg, Fe, Zn and Cu) and Non-Essential (Cd and Pb) Elements in Predatory Insects (<i>Vespa crabro</i> and <i>Vespa velutina</i>): A Molecular Perspective |
| title_full_unstemmed | Essential (Mg, Fe, Zn and Cu) and Non-Essential (Cd and Pb) Elements in Predatory Insects (<i>Vespa crabro</i> and <i>Vespa velutina</i>): A Molecular Perspective |
| title_short | Essential (Mg, Fe, Zn and Cu) and Non-Essential (Cd and Pb) Elements in Predatory Insects (<i>Vespa crabro</i> and <i>Vespa velutina</i>): A Molecular Perspective |
| title_sort | essential mg fe zn and cu and non essential cd and pb elements in predatory insects i vespa crabro i and i vespa velutina i a molecular perspective |
| topic | Asian yellow-legged hornet European hornet electrophoresis proteomics iron copper |
| url | https://www.mdpi.com/1422-0067/22/1/228 |
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