Preparation of High Mechanical Strength Chitosan Nanofiber/NanoSiO<sub>2</sub>/PVA Composite Scaffolds for Bone Tissue Engineering Using Sol–Gel Method
The majority of chitosan-based bone tissue engineering (BTE) scaffolds have the problem of poor mechanical properties. However, modifying chitosan with conventional silane coupling agents to improve the mechanical properties of scaffolds will introduce additional complications, including cytotoxicit...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/10/2083 |
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| author | Wei Ma Sihan Zhang Chong Xie Xing Wan Xiaofeng Li Kebing Chen Guanglei Zhao |
| author_facet | Wei Ma Sihan Zhang Chong Xie Xing Wan Xiaofeng Li Kebing Chen Guanglei Zhao |
| author_sort | Wei Ma |
| collection | DOAJ |
| description | The majority of chitosan-based bone tissue engineering (BTE) scaffolds have the problem of poor mechanical properties. However, modifying chitosan with conventional silane coupling agents to improve the mechanical properties of scaffolds will introduce additional complications, including cytotoxicity and poor biocompatibility. In this study, two types of organic–inorganic composite scaffolds (F-A-T0/T3/T5 and F-B-T5-P0/P0.5/P1.5/P2.5) were prepared using chitosan nanofibers (CSNF) prepared by the beating-homogenization method, combined with the sol–gel method, and further introduced polyvinyl alcohol (PVA). The F-A-T3 and F-B-T5-P1.5 exhibited interconnected pore and surface nanofibers structures, high porosity (>70%), outstanding swelling properties, and a controllable degradation rate. The Young’s modulus of TEOS: 5.0% (<i>w</i>/<i>w</i>), PVA: 1.5% (<i>w</i>/<i>w</i>) chitosan fiber scaffold is 8.53 ± 0.43 MPa in dry conditions, and 237.78 ± 8.86 kPa in wet conditions, which is four times that of F-A-T5 and twice that of F-B-T5-P0. Additionally, cell (MC3T3-E1) experiments confirmed that the two composite scaffolds had great cytocompatibility and were predicted to be used in the future in the field of BTE scaffolds. |
| first_indexed | 2024-03-10T03:00:45Z |
| format | Article |
| id | doaj.art-5b40d2bf71964a3398e3ddd38a82ea0d |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T03:00:45Z |
| publishDate | 2022-05-01 |
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| series | Polymers |
| spelling | doaj.art-5b40d2bf71964a3398e3ddd38a82ea0d2023-11-23T12:47:12ZengMDPI AGPolymers2073-43602022-05-011410208310.3390/polym14102083Preparation of High Mechanical Strength Chitosan Nanofiber/NanoSiO<sub>2</sub>/PVA Composite Scaffolds for Bone Tissue Engineering Using Sol–Gel MethodWei Ma0Sihan Zhang1Chong Xie2Xing Wan3Xiaofeng Li4Kebing Chen5Guanglei Zhao6State Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510641, ChinaState Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510641, ChinaState Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510641, ChinaState Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510641, ChinaState Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510641, ChinaDepartment of Spine Surgery, The Sixth Affiliated Hospital of Sun Yat-sen University, 26 Erheng Road, Yuan Village, Guangzhou, 510655, ChinaState Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510641, ChinaThe majority of chitosan-based bone tissue engineering (BTE) scaffolds have the problem of poor mechanical properties. However, modifying chitosan with conventional silane coupling agents to improve the mechanical properties of scaffolds will introduce additional complications, including cytotoxicity and poor biocompatibility. In this study, two types of organic–inorganic composite scaffolds (F-A-T0/T3/T5 and F-B-T5-P0/P0.5/P1.5/P2.5) were prepared using chitosan nanofibers (CSNF) prepared by the beating-homogenization method, combined with the sol–gel method, and further introduced polyvinyl alcohol (PVA). The F-A-T3 and F-B-T5-P1.5 exhibited interconnected pore and surface nanofibers structures, high porosity (>70%), outstanding swelling properties, and a controllable degradation rate. The Young’s modulus of TEOS: 5.0% (<i>w</i>/<i>w</i>), PVA: 1.5% (<i>w</i>/<i>w</i>) chitosan fiber scaffold is 8.53 ± 0.43 MPa in dry conditions, and 237.78 ± 8.86 kPa in wet conditions, which is four times that of F-A-T5 and twice that of F-B-T5-P0. Additionally, cell (MC3T3-E1) experiments confirmed that the two composite scaffolds had great cytocompatibility and were predicted to be used in the future in the field of BTE scaffolds.https://www.mdpi.com/2073-4360/14/10/2083bone tissue engineeringnanofiberchitosansol–gelPVA |
| spellingShingle | Wei Ma Sihan Zhang Chong Xie Xing Wan Xiaofeng Li Kebing Chen Guanglei Zhao Preparation of High Mechanical Strength Chitosan Nanofiber/NanoSiO<sub>2</sub>/PVA Composite Scaffolds for Bone Tissue Engineering Using Sol–Gel Method Polymers bone tissue engineering nanofiber chitosan sol–gel PVA |
| title | Preparation of High Mechanical Strength Chitosan Nanofiber/NanoSiO<sub>2</sub>/PVA Composite Scaffolds for Bone Tissue Engineering Using Sol–Gel Method |
| title_full | Preparation of High Mechanical Strength Chitosan Nanofiber/NanoSiO<sub>2</sub>/PVA Composite Scaffolds for Bone Tissue Engineering Using Sol–Gel Method |
| title_fullStr | Preparation of High Mechanical Strength Chitosan Nanofiber/NanoSiO<sub>2</sub>/PVA Composite Scaffolds for Bone Tissue Engineering Using Sol–Gel Method |
| title_full_unstemmed | Preparation of High Mechanical Strength Chitosan Nanofiber/NanoSiO<sub>2</sub>/PVA Composite Scaffolds for Bone Tissue Engineering Using Sol–Gel Method |
| title_short | Preparation of High Mechanical Strength Chitosan Nanofiber/NanoSiO<sub>2</sub>/PVA Composite Scaffolds for Bone Tissue Engineering Using Sol–Gel Method |
| title_sort | preparation of high mechanical strength chitosan nanofiber nanosio sub 2 sub pva composite scaffolds for bone tissue engineering using sol gel method |
| topic | bone tissue engineering nanofiber chitosan sol–gel PVA |
| url | https://www.mdpi.com/2073-4360/14/10/2083 |
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