Non-extractive spectrophotometric determination of valsartan in pure form and in pharmaceutical products by ion-pair complex formation with bromophenol blue and methyl red
Two simple, rapid, green non extractive spectrophotometric methods are described for the estimation of valsartan in tablet dosage form. The determination is based on the ion-pair formation using the dyes, bromophenol blue (BPB) and methyl red (MR). Valsartan forms ion-pair complex selectively with t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2021-11-01
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| Series: | Pharmacia |
| Online Access: | https://pharmacia.pensoft.net/article/73559/download/pdf/ |
| _version_ | 1818337200036642816 |
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| author | Kateryna Peleshok Bohdana Bondar Liubomyr Kryskiw Tetyana Kucher Olha Poliak Liliya Logoyda |
| author_facet | Kateryna Peleshok Bohdana Bondar Liubomyr Kryskiw Tetyana Kucher Olha Poliak Liliya Logoyda |
| author_sort | Kateryna Peleshok |
| collection | DOAJ |
| description | Two simple, rapid, green non extractive spectrophotometric methods are described for the estimation of valsartan in tablet dosage form. The determination is based on the ion-pair formation using the dyes, bromophenol blue (BPB) and methyl red (MR). Valsartan forms ion-pair complex selectively with the dyes, as indicated by the formation of a coloured complex with BPB at pH 5.5 with λmax at 424 nm and MR at pH 4.3 with λmax at 494 nm. For both methods, optimal spectrophotometric conditions were established. The linear relationship was found between absorbance at λmax and concentration of drug in the range 8–24 µg/mL for BPB and 4–20 µg/mL for MR. Regression analysis of Beer’s law plot at 424 nm yielded the regression equation, y = 0.0102x + 01636 (BPB) and at 494 nm y = 0.0222x – 0.0063 (MR). High values of correlations coefficient (R2 = 0.9988 (BPB) and R2 = 0.9991 (MR)) and small values of intercept validated the linearity of calibration curve and obedience to Beer’s law. The LOD and LOQ values were calculated to be 1.03 µg/mL and 3.43 µg/mL respectively (BPB) and 0.68 µg/mL and 2.26 µg/mL respectively (MR). Intra-day and inter-day accuracy and precision, robustness were in acceptable limits. The proposed methods were applied for the quantification of valsartan in tablets pertaining to three commercial formulations. Analytical eco-scale for greenness assessment of the proposed spectrophotometric methods showed that both methods corresponds to excellent green analysis with a score of 89. |
| first_indexed | 2024-12-13T14:51:26Z |
| format | Article |
| id | doaj.art-5b4161833db34ea5b68d2bf2913874d7 |
| institution | Directory Open Access Journal |
| issn | 2603-557X |
| language | English |
| last_indexed | 2024-12-13T14:51:26Z |
| publishDate | 2021-11-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Pharmacia |
| spelling | doaj.art-5b4161833db34ea5b68d2bf2913874d72022-12-21T23:41:20ZengPensoft PublishersPharmacia2603-557X2021-11-0168485185810.3897/pharmacia.68.e7355973559Non-extractive spectrophotometric determination of valsartan in pure form and in pharmaceutical products by ion-pair complex formation with bromophenol blue and methyl redKateryna Peleshok0Bohdana Bondar1Liubomyr Kryskiw2Tetyana Kucher3Olha Poliak4Liliya Logoyda5I. Horbachevsky Ternopil National Medical UniversityI. Horbachevsky Ternopil National Medical UniversityI. Horbachevsky Ternopil National Medical UniversityI. Horbachevsky Ternopil National Medical UniversityI. Horbachevsky Ternopil National Medical UniversityI. Horbachevsky Ternopil National Medical UniversityTwo simple, rapid, green non extractive spectrophotometric methods are described for the estimation of valsartan in tablet dosage form. The determination is based on the ion-pair formation using the dyes, bromophenol blue (BPB) and methyl red (MR). Valsartan forms ion-pair complex selectively with the dyes, as indicated by the formation of a coloured complex with BPB at pH 5.5 with λmax at 424 nm and MR at pH 4.3 with λmax at 494 nm. For both methods, optimal spectrophotometric conditions were established. The linear relationship was found between absorbance at λmax and concentration of drug in the range 8–24 µg/mL for BPB and 4–20 µg/mL for MR. Regression analysis of Beer’s law plot at 424 nm yielded the regression equation, y = 0.0102x + 01636 (BPB) and at 494 nm y = 0.0222x – 0.0063 (MR). High values of correlations coefficient (R2 = 0.9988 (BPB) and R2 = 0.9991 (MR)) and small values of intercept validated the linearity of calibration curve and obedience to Beer’s law. The LOD and LOQ values were calculated to be 1.03 µg/mL and 3.43 µg/mL respectively (BPB) and 0.68 µg/mL and 2.26 µg/mL respectively (MR). Intra-day and inter-day accuracy and precision, robustness were in acceptable limits. The proposed methods were applied for the quantification of valsartan in tablets pertaining to three commercial formulations. Analytical eco-scale for greenness assessment of the proposed spectrophotometric methods showed that both methods corresponds to excellent green analysis with a score of 89.https://pharmacia.pensoft.net/article/73559/download/pdf/ |
| spellingShingle | Kateryna Peleshok Bohdana Bondar Liubomyr Kryskiw Tetyana Kucher Olha Poliak Liliya Logoyda Non-extractive spectrophotometric determination of valsartan in pure form and in pharmaceutical products by ion-pair complex formation with bromophenol blue and methyl red Pharmacia |
| title | Non-extractive spectrophotometric determination of valsartan in pure form and in pharmaceutical products by ion-pair complex formation with bromophenol blue and methyl red |
| title_full | Non-extractive spectrophotometric determination of valsartan in pure form and in pharmaceutical products by ion-pair complex formation with bromophenol blue and methyl red |
| title_fullStr | Non-extractive spectrophotometric determination of valsartan in pure form and in pharmaceutical products by ion-pair complex formation with bromophenol blue and methyl red |
| title_full_unstemmed | Non-extractive spectrophotometric determination of valsartan in pure form and in pharmaceutical products by ion-pair complex formation with bromophenol blue and methyl red |
| title_short | Non-extractive spectrophotometric determination of valsartan in pure form and in pharmaceutical products by ion-pair complex formation with bromophenol blue and methyl red |
| title_sort | non extractive spectrophotometric determination of valsartan in pure form and in pharmaceutical products by ion pair complex formation with bromophenol blue and methyl red |
| url | https://pharmacia.pensoft.net/article/73559/download/pdf/ |
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