Predictive value of peripheral blood leukocytes-based methylation of Long non-coding RNA MALAT1 and H19 in the chemotherapy effect and prognosis of gastric cancer
Background: The predictive value of the methylation of Long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and H19 promoters in peripheral blood leukocytes as a non-invasive biomarker for the chemotherapy effect and prognosis gastric cancer (GC) is unclear. M...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Translational Oncology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523324000561 |
| _version_ | 1827315542211428352 |
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| author | Fang Wang Dingtao Hu Xiaoqi Lou Linlin Wang Yuhua Wang Tingyu Zhang Ziye Yan Nana Meng Yu Lei Yanfeng Zou |
| author_facet | Fang Wang Dingtao Hu Xiaoqi Lou Linlin Wang Yuhua Wang Tingyu Zhang Ziye Yan Nana Meng Yu Lei Yanfeng Zou |
| author_sort | Fang Wang |
| collection | DOAJ |
| description | Background: The predictive value of the methylation of Long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and H19 promoters in peripheral blood leukocytes as a non-invasive biomarker for the chemotherapy effect and prognosis gastric cancer (GC) is unclear. Methods: The DNA methylation of H19 and MALAT1 between chemotherapy-sensitive and non-sensitive groups and between groups with better and worse survival of GC was compared using regression analyses. Several predictive nomograms were constructed. The genetic alteration of MALAT1 and H19 and the association between gene expression and immune status in GC were also investigated using bioinformatics analysis. Results: Higher genetic methylations in peripheral blood were noticed in GC groups with poorer survival. The constructed nomograms presented strong predictive values for the chemotherapy effect and 3-year survival of disease-free survival, progression-free survival, and overall survival, with the area under the curve as 0.838, 0.838, 0.912, and 0.925, respectively. Significant correlations between MALAT1 or H19 expression and marker genes of immune checkpoints and immune pathways were noticed. The high infiltration of macrophages in H19-high and low infiltration of CD8+ T cells in MALAT1-high groups were associated with worse survival of GC. Conclusions: MALAT1 and H19 have the potential to predict the chemotherapy response and clinical outcomes of GC. |
| first_indexed | 2024-04-24T22:58:04Z |
| format | Article |
| id | doaj.art-5b43839d3c3942b58a8cba63dae3e107 |
| institution | Directory Open Access Journal |
| issn | 1936-5233 |
| language | English |
| last_indexed | 2024-04-24T22:58:04Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj.art-5b43839d3c3942b58a8cba63dae3e1072024-03-18T04:33:53ZengElsevierTranslational Oncology1936-52332024-06-0144101929Predictive value of peripheral blood leukocytes-based methylation of Long non-coding RNA MALAT1 and H19 in the chemotherapy effect and prognosis of gastric cancerFang Wang0Dingtao Hu1Xiaoqi Lou2Linlin Wang3Yuhua Wang4Tingyu Zhang5Ziye Yan6Nana Meng7Yu Lei8Yanfeng Zou9Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, PR China; Corresponding author. Department of Oncology, The First Affiliated Hospital of Anhui Medical University, 218Jixi Road, Hefei 230022, Anhui, PR China.Clinical Cancer Institute, Center for Translational Medicine, Naval Medical University, Shanghai 200433, PR ChinaDepartment of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, PR ChinaDepartment of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, Anhui, PR ChinaDepartment of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, Anhui, PR ChinaDepartment of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, Anhui, PR ChinaDepartment of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, Anhui, PR ChinaDepartment of Quality Management Office, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, PR ChinaDepartment of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, PR ChinaDepartment of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, Anhui, PR ChinaBackground: The predictive value of the methylation of Long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and H19 promoters in peripheral blood leukocytes as a non-invasive biomarker for the chemotherapy effect and prognosis gastric cancer (GC) is unclear. Methods: The DNA methylation of H19 and MALAT1 between chemotherapy-sensitive and non-sensitive groups and between groups with better and worse survival of GC was compared using regression analyses. Several predictive nomograms were constructed. The genetic alteration of MALAT1 and H19 and the association between gene expression and immune status in GC were also investigated using bioinformatics analysis. Results: Higher genetic methylations in peripheral blood were noticed in GC groups with poorer survival. The constructed nomograms presented strong predictive values for the chemotherapy effect and 3-year survival of disease-free survival, progression-free survival, and overall survival, with the area under the curve as 0.838, 0.838, 0.912, and 0.925, respectively. Significant correlations between MALAT1 or H19 expression and marker genes of immune checkpoints and immune pathways were noticed. The high infiltration of macrophages in H19-high and low infiltration of CD8+ T cells in MALAT1-high groups were associated with worse survival of GC. Conclusions: MALAT1 and H19 have the potential to predict the chemotherapy response and clinical outcomes of GC.http://www.sciencedirect.com/science/article/pii/S1936523324000561Gastric cancerMalat1H19MethylationPrognosis |
| spellingShingle | Fang Wang Dingtao Hu Xiaoqi Lou Linlin Wang Yuhua Wang Tingyu Zhang Ziye Yan Nana Meng Yu Lei Yanfeng Zou Predictive value of peripheral blood leukocytes-based methylation of Long non-coding RNA MALAT1 and H19 in the chemotherapy effect and prognosis of gastric cancer Translational Oncology Gastric cancer Malat1 H19 Methylation Prognosis |
| title | Predictive value of peripheral blood leukocytes-based methylation of Long non-coding RNA MALAT1 and H19 in the chemotherapy effect and prognosis of gastric cancer |
| title_full | Predictive value of peripheral blood leukocytes-based methylation of Long non-coding RNA MALAT1 and H19 in the chemotherapy effect and prognosis of gastric cancer |
| title_fullStr | Predictive value of peripheral blood leukocytes-based methylation of Long non-coding RNA MALAT1 and H19 in the chemotherapy effect and prognosis of gastric cancer |
| title_full_unstemmed | Predictive value of peripheral blood leukocytes-based methylation of Long non-coding RNA MALAT1 and H19 in the chemotherapy effect and prognosis of gastric cancer |
| title_short | Predictive value of peripheral blood leukocytes-based methylation of Long non-coding RNA MALAT1 and H19 in the chemotherapy effect and prognosis of gastric cancer |
| title_sort | predictive value of peripheral blood leukocytes based methylation of long non coding rna malat1 and h19 in the chemotherapy effect and prognosis of gastric cancer |
| topic | Gastric cancer Malat1 H19 Methylation Prognosis |
| url | http://www.sciencedirect.com/science/article/pii/S1936523324000561 |
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