Comparative efficacy of a novel Bacillus subtilis-based probiotic and pharmacological zinc oxide on growth performance and gut responses in nursery pigs
Abstract In this study, we assessed the efficacy of a novel Bacillus subtilis probiotic in improving growth performance and gut responses in comparison to pharmacological zinc oxide (ZnO) in nursery pigs. A total of 96 piglets were randomly assigned to four groups: Negative control (NC), Positive co...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-31913-0 |
| _version_ | 1827979509024948224 |
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| author | Sudhanshu Sudan Lauren Fletcher Xiaoshu Zhan Serena Dingle Rob Patterson Lee-Anne Huber Robert Friendship Elijah G. Kiarie Julang Li |
| author_facet | Sudhanshu Sudan Lauren Fletcher Xiaoshu Zhan Serena Dingle Rob Patterson Lee-Anne Huber Robert Friendship Elijah G. Kiarie Julang Li |
| author_sort | Sudhanshu Sudan |
| collection | DOAJ |
| description | Abstract In this study, we assessed the efficacy of a novel Bacillus subtilis probiotic in improving growth performance and gut responses in comparison to pharmacological zinc oxide (ZnO) in nursery pigs. A total of 96 piglets were randomly assigned to four groups: Negative control (NC), Positive control (PC, 3000 mg Zn /kg feed), B.subtilis low dose (BS9-L, 2 × 107 CFU/pig) and B.subtilis high dose (BS9-H, 2 × 109 CFU/pig). Growth performance, diarrhea rate, gut mucosal gene expression and fecal microbial populations were evaluated. B.subtilis administration did not improve piglet bodyweight. BS9-L showed (P < 0.05) higher average daily gain (ADG) in Period 2 (D14-D28). BS9 groups had (P < 0.001) lower feed conversion ratio (FCR) in Period 2 (D14-D28) and overall. Like the ZnO-group, BS9 groups had lower (P < 0.01) diarrhea rate. A significant reduction (P < 0.05) in fecal E. coli, total coliforms, and an increase in lactic acid bacteria and Bacillus spp. in BS9 groups was observed. BS9 group had reduced (P < 0.05) mRNA levels of intestinal IL-8 and higher levels of MUC-1 and occludin and TJP-1 compared to negative control. These findings suggest that probiotic BS9, may promote growth performance, and ameliorate various indicators of intestinal health in piglets. Hence, it may serve as a prospective alternative to ZnO growth promoter in commercial swine production. |
| first_indexed | 2024-04-09T21:37:53Z |
| format | Article |
| id | doaj.art-5b46f069ccdd4903b60003858efd335b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T21:37:53Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5b46f069ccdd4903b60003858efd335b2023-03-26T11:09:58ZengNature PortfolioScientific Reports2045-23222023-03-0113111210.1038/s41598-023-31913-0Comparative efficacy of a novel Bacillus subtilis-based probiotic and pharmacological zinc oxide on growth performance and gut responses in nursery pigsSudhanshu Sudan0Lauren Fletcher1Xiaoshu Zhan2Serena Dingle3Rob Patterson4Lee-Anne Huber5Robert Friendship6Elijah G. Kiarie7Julang Li8Department of Animal Biosciences, University of GuelphDepartment of Animal Biosciences, University of GuelphDepartment of Animal Biosciences, University of GuelphDepartment of Animal Biosciences, University of GuelphCBS Bio Platforms IncDepartment of Animal Biosciences, University of GuelphDepartment of Population Medicine, University of GuelphDepartment of Animal Biosciences, University of GuelphDepartment of Animal Biosciences, University of GuelphAbstract In this study, we assessed the efficacy of a novel Bacillus subtilis probiotic in improving growth performance and gut responses in comparison to pharmacological zinc oxide (ZnO) in nursery pigs. A total of 96 piglets were randomly assigned to four groups: Negative control (NC), Positive control (PC, 3000 mg Zn /kg feed), B.subtilis low dose (BS9-L, 2 × 107 CFU/pig) and B.subtilis high dose (BS9-H, 2 × 109 CFU/pig). Growth performance, diarrhea rate, gut mucosal gene expression and fecal microbial populations were evaluated. B.subtilis administration did not improve piglet bodyweight. BS9-L showed (P < 0.05) higher average daily gain (ADG) in Period 2 (D14-D28). BS9 groups had (P < 0.001) lower feed conversion ratio (FCR) in Period 2 (D14-D28) and overall. Like the ZnO-group, BS9 groups had lower (P < 0.01) diarrhea rate. A significant reduction (P < 0.05) in fecal E. coli, total coliforms, and an increase in lactic acid bacteria and Bacillus spp. in BS9 groups was observed. BS9 group had reduced (P < 0.05) mRNA levels of intestinal IL-8 and higher levels of MUC-1 and occludin and TJP-1 compared to negative control. These findings suggest that probiotic BS9, may promote growth performance, and ameliorate various indicators of intestinal health in piglets. Hence, it may serve as a prospective alternative to ZnO growth promoter in commercial swine production.https://doi.org/10.1038/s41598-023-31913-0 |
| spellingShingle | Sudhanshu Sudan Lauren Fletcher Xiaoshu Zhan Serena Dingle Rob Patterson Lee-Anne Huber Robert Friendship Elijah G. Kiarie Julang Li Comparative efficacy of a novel Bacillus subtilis-based probiotic and pharmacological zinc oxide on growth performance and gut responses in nursery pigs Scientific Reports |
| title | Comparative efficacy of a novel Bacillus subtilis-based probiotic and pharmacological zinc oxide on growth performance and gut responses in nursery pigs |
| title_full | Comparative efficacy of a novel Bacillus subtilis-based probiotic and pharmacological zinc oxide on growth performance and gut responses in nursery pigs |
| title_fullStr | Comparative efficacy of a novel Bacillus subtilis-based probiotic and pharmacological zinc oxide on growth performance and gut responses in nursery pigs |
| title_full_unstemmed | Comparative efficacy of a novel Bacillus subtilis-based probiotic and pharmacological zinc oxide on growth performance and gut responses in nursery pigs |
| title_short | Comparative efficacy of a novel Bacillus subtilis-based probiotic and pharmacological zinc oxide on growth performance and gut responses in nursery pigs |
| title_sort | comparative efficacy of a novel bacillus subtilis based probiotic and pharmacological zinc oxide on growth performance and gut responses in nursery pigs |
| url | https://doi.org/10.1038/s41598-023-31913-0 |
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