Chemical Composition, Antioxidant and Anti-Enzymatic Activity of Golden Root (<i>Rhodiola rosea</i> L.) Commercial Samples
The aim of the study was to compare the chemical composition of the water and hydromethanolic extracts of <i>R. rosea</i> commercial samples in relation to their biological activity. For this purpose, the HPLC method was used for the determination of eleven phenolic compounds and AAS/AES...
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MDPI AG
2022-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/5/919 |
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| author | Milena Polumackanycz Pawel Konieczynski Ilkay Erdogan Orhan Nurten Abaci Agnieszka Viapiana |
| author_facet | Milena Polumackanycz Pawel Konieczynski Ilkay Erdogan Orhan Nurten Abaci Agnieszka Viapiana |
| author_sort | Milena Polumackanycz |
| collection | DOAJ |
| description | The aim of the study was to compare the chemical composition of the water and hydromethanolic extracts of <i>R. rosea</i> commercial samples in relation to their biological activity. For this purpose, the HPLC method was used for the determination of eleven phenolic compounds and AAS/AES was used for determination of five essential elements. Moreover, the contents of total phenolic, total flavonoid, total phenolic acids, and L(+)-ascorbic acid were determined. The antioxidant activity was assessed by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS radical scavenging activity, ferric-reducing/antioxidant power (FRAP), and cupric-reducing antioxidant capacity (CUPRAC) assays, while the inhibitory activity against AChE and BChE enzymes was determined using Ellman’s method. The results showed that the hydromethanolic extracts of <i>R. rosea</i> were richer in phenolic compounds and showed higher antioxidant and neurobiological activity than the water extracts. However, the water extracts gave higher contents of determined elements. Among the individual phenolic compounds gallic acid (2.33 mg/g DW) and sinapic acid (386.44 µg/g DW) had the highest concentrations in the hydromethanolic and water extracts, respectively. Moreover, the most extracts were observed to be more efficient on BChE. Moreover, the correlation analysis indicated a high positive relationship between chemical composition and biological activity in both extracts of <i>R. rosea</i>. |
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| format | Article |
| id | doaj.art-5b486cf9e6b94e16a37057605ccb24a3 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T03:26:18Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-5b486cf9e6b94e16a37057605ccb24a32023-11-23T09:51:43ZengMDPI AGAntioxidants2076-39212022-05-0111591910.3390/antiox11050919Chemical Composition, Antioxidant and Anti-Enzymatic Activity of Golden Root (<i>Rhodiola rosea</i> L.) Commercial SamplesMilena Polumackanycz0Pawel Konieczynski1Ilkay Erdogan Orhan2Nurten Abaci3Agnieszka Viapiana4Department of Analytical Chemistry, Medical University of Gdansk, Gen. J. Hallera 107, 80-416 Gdansk, PolandDepartment of Analytical Chemistry, Medical University of Gdansk, Gen. J. Hallera 107, 80-416 Gdansk, PolandDepartment of Pharmacognosy, Faculty of Pharmacy, Gazi University, Ankara 06330, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Gazi University, Ankara 06330, TurkeyDepartment of Analytical Chemistry, Medical University of Gdansk, Gen. J. Hallera 107, 80-416 Gdansk, PolandThe aim of the study was to compare the chemical composition of the water and hydromethanolic extracts of <i>R. rosea</i> commercial samples in relation to their biological activity. For this purpose, the HPLC method was used for the determination of eleven phenolic compounds and AAS/AES was used for determination of five essential elements. Moreover, the contents of total phenolic, total flavonoid, total phenolic acids, and L(+)-ascorbic acid were determined. The antioxidant activity was assessed by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS radical scavenging activity, ferric-reducing/antioxidant power (FRAP), and cupric-reducing antioxidant capacity (CUPRAC) assays, while the inhibitory activity against AChE and BChE enzymes was determined using Ellman’s method. The results showed that the hydromethanolic extracts of <i>R. rosea</i> were richer in phenolic compounds and showed higher antioxidant and neurobiological activity than the water extracts. However, the water extracts gave higher contents of determined elements. Among the individual phenolic compounds gallic acid (2.33 mg/g DW) and sinapic acid (386.44 µg/g DW) had the highest concentrations in the hydromethanolic and water extracts, respectively. Moreover, the most extracts were observed to be more efficient on BChE. Moreover, the correlation analysis indicated a high positive relationship between chemical composition and biological activity in both extracts of <i>R. rosea</i>.https://www.mdpi.com/2076-3921/11/5/919<i>Rhodiola rosea</i>antioxidant activityphenolic compositionelementsneurobiological activity |
| spellingShingle | Milena Polumackanycz Pawel Konieczynski Ilkay Erdogan Orhan Nurten Abaci Agnieszka Viapiana Chemical Composition, Antioxidant and Anti-Enzymatic Activity of Golden Root (<i>Rhodiola rosea</i> L.) Commercial Samples Antioxidants <i>Rhodiola rosea</i> antioxidant activity phenolic composition elements neurobiological activity |
| title | Chemical Composition, Antioxidant and Anti-Enzymatic Activity of Golden Root (<i>Rhodiola rosea</i> L.) Commercial Samples |
| title_full | Chemical Composition, Antioxidant and Anti-Enzymatic Activity of Golden Root (<i>Rhodiola rosea</i> L.) Commercial Samples |
| title_fullStr | Chemical Composition, Antioxidant and Anti-Enzymatic Activity of Golden Root (<i>Rhodiola rosea</i> L.) Commercial Samples |
| title_full_unstemmed | Chemical Composition, Antioxidant and Anti-Enzymatic Activity of Golden Root (<i>Rhodiola rosea</i> L.) Commercial Samples |
| title_short | Chemical Composition, Antioxidant and Anti-Enzymatic Activity of Golden Root (<i>Rhodiola rosea</i> L.) Commercial Samples |
| title_sort | chemical composition antioxidant and anti enzymatic activity of golden root i rhodiola rosea i l commercial samples |
| topic | <i>Rhodiola rosea</i> antioxidant activity phenolic composition elements neurobiological activity |
| url | https://www.mdpi.com/2076-3921/11/5/919 |
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