MEOX2 Transcription Factor Is Involved in Survival and Adhesion of Glioma Stem-like Cells
The high expression of MEOX2 transcription factor is closely associated with poor overall survival in glioma. MEOX2 has recently been described as an interesting prognostic biomarker, especially for lower grade glioma. MEOX2 has never been studied in glioma stem-like cells (GSC), responsible for gli...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/13/23/5943 |
| _version_ | 1797507982918942720 |
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| author | Gaëlle Tachon Konstantin Masliantsev Pierre Rivet Amandine Desette Serge Milin Elise Gueret Michel Wager Lucie Karayan-Tapon Pierre-Olivier Guichet |
| author_facet | Gaëlle Tachon Konstantin Masliantsev Pierre Rivet Amandine Desette Serge Milin Elise Gueret Michel Wager Lucie Karayan-Tapon Pierre-Olivier Guichet |
| author_sort | Gaëlle Tachon |
| collection | DOAJ |
| description | The high expression of MEOX2 transcription factor is closely associated with poor overall survival in glioma. MEOX2 has recently been described as an interesting prognostic biomarker, especially for lower grade glioma. MEOX2 has never been studied in glioma stem-like cells (GSC), responsible for glioma recurrence. The aim of our study was to investigate the role of MEOX2 in GSC. Loss of function approach using siRNA was used to assess the impact of MEOX2 on GSC viability and stemness phenotype. MEOX2 was localized in the nucleus and its expression was heterogeneous between GSCs. MEOX2 expression depends on the methylation state of its promoter and is strongly associated with <i>IDH</i> mutations. MEOX2 is involved in cell proliferation and viability regulation through ERK/MAPK and PI3K/AKT pathways. MEOX2 loss of function correlated with GSC differentiation and acquisition of neuronal lineage characteristics. Besides, inhibition of MEOX2 is correlated with increased expression of CDH10 and decreased pFAK. In this study, we unraveled, for the first time, MEOX2 contribution to cell viability and proliferation through AKT/ERK pathway and its potential involvement in phenotype and adhesion properties of GSC. |
| first_indexed | 2024-03-10T04:56:08Z |
| format | Article |
| id | doaj.art-5b4d49ffb4484d5cb963230a23273b83 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T04:56:08Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-5b4d49ffb4484d5cb963230a23273b832023-11-23T02:11:53ZengMDPI AGCancers2072-66942021-11-011323594310.3390/cancers13235943MEOX2 Transcription Factor Is Involved in Survival and Adhesion of Glioma Stem-like CellsGaëlle Tachon0Konstantin Masliantsev1Pierre Rivet2Amandine Desette3Serge Milin4Elise Gueret5Michel Wager6Lucie Karayan-Tapon7Pierre-Olivier Guichet8Université de Poitiers, CHU Poitiers, ProDiCeT, 86000 Poitiers, FranceUniversité de Poitiers, CHU Poitiers, ProDiCeT, 86000 Poitiers, FranceLaboratoire de Cancérologie Biologique, CHU Poitiers, 86000 Poitiers, FranceUniversité de Poitiers, CHU Poitiers, ProDiCeT, 86000 Poitiers, FranceService d’Anatomo-Cytopathologie, CHU Poitiers, 86000 Poitiers, FranceUniversité Montpellier, CNRS, INSERM, 34094 Montpellier, FranceUniversité de Poitiers, CHU Poitiers, ProDiCeT, 86000 Poitiers, FranceUniversité de Poitiers, CHU Poitiers, ProDiCeT, 86000 Poitiers, FranceUniversité de Poitiers, CHU Poitiers, ProDiCeT, 86000 Poitiers, FranceThe high expression of MEOX2 transcription factor is closely associated with poor overall survival in glioma. MEOX2 has recently been described as an interesting prognostic biomarker, especially for lower grade glioma. MEOX2 has never been studied in glioma stem-like cells (GSC), responsible for glioma recurrence. The aim of our study was to investigate the role of MEOX2 in GSC. Loss of function approach using siRNA was used to assess the impact of MEOX2 on GSC viability and stemness phenotype. MEOX2 was localized in the nucleus and its expression was heterogeneous between GSCs. MEOX2 expression depends on the methylation state of its promoter and is strongly associated with <i>IDH</i> mutations. MEOX2 is involved in cell proliferation and viability regulation through ERK/MAPK and PI3K/AKT pathways. MEOX2 loss of function correlated with GSC differentiation and acquisition of neuronal lineage characteristics. Besides, inhibition of MEOX2 is correlated with increased expression of CDH10 and decreased pFAK. In this study, we unraveled, for the first time, MEOX2 contribution to cell viability and proliferation through AKT/ERK pathway and its potential involvement in phenotype and adhesion properties of GSC.https://www.mdpi.com/2072-6694/13/23/5943MEOX2glioma stem-like cellsdifferentiationERKCDH10 |
| spellingShingle | Gaëlle Tachon Konstantin Masliantsev Pierre Rivet Amandine Desette Serge Milin Elise Gueret Michel Wager Lucie Karayan-Tapon Pierre-Olivier Guichet MEOX2 Transcription Factor Is Involved in Survival and Adhesion of Glioma Stem-like Cells Cancers MEOX2 glioma stem-like cells differentiation ERK CDH10 |
| title | MEOX2 Transcription Factor Is Involved in Survival and Adhesion of Glioma Stem-like Cells |
| title_full | MEOX2 Transcription Factor Is Involved in Survival and Adhesion of Glioma Stem-like Cells |
| title_fullStr | MEOX2 Transcription Factor Is Involved in Survival and Adhesion of Glioma Stem-like Cells |
| title_full_unstemmed | MEOX2 Transcription Factor Is Involved in Survival and Adhesion of Glioma Stem-like Cells |
| title_short | MEOX2 Transcription Factor Is Involved in Survival and Adhesion of Glioma Stem-like Cells |
| title_sort | meox2 transcription factor is involved in survival and adhesion of glioma stem like cells |
| topic | MEOX2 glioma stem-like cells differentiation ERK CDH10 |
| url | https://www.mdpi.com/2072-6694/13/23/5943 |
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